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fluoroshield mounting medium with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield mounting medium with dapi
    Fluoroshield Mounting Medium With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+dapi/DAPI/pmc13124985-211-29-34
    Average 99 stars, based on 32727 article reviews
    fluoroshield mounting medium with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
    Article Snippet: NIH‐3T3 cells, RAW264.7 cells, or 4T1/MCF7 cells treated with 40 ng mL −1 rCXCL10 were seeded onto the coverslips and cultured for 72 h. Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.2% Triton X‐100 (Solarbio, T8200) for 5 min, and blocked with 5% BSA (Solarbio, A8850) for 2 h. For tissue sections, slides underwent deparaffinization, rehydration, and blocking with 5% BSA for 2 h. Both coverslips and tissue sections were incubated overnight at 4°C with primary antibodies against α‐SMA (Affinity, AF1032), CD206 (Affinity, DF4149), or β‐Catenin (Proteintech, PRO‐CY‐50WY). .. After three washes, samples were incubated for 1 h at room temperature in the dark with Fluor488‐conjugated secondary antibody (Immunoway, RS32109), counterstained with DAPI (Beyotime, C1005) for 3 min, and mounted with antifade medium (Abbkine, BMU104). ..

    Lysis:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: Mouse ELISA kits for quantifying IL‐6, IL‐1β, TNF‐α, and IFN‐β were supplied by Beijing Solarbio Science & Technology Co., Ltd. (China). .. DAPI, PMSF, and RIPA lysis buffer were acquired from Beyotime Biotechnology (China). .. Antibodies targeting phospho‐TBK1 (Ser172, #5483), phospho‐STING (Ser365, #72971), total TBK1 (#38066), and total STING (#13647) were purchased from Cell Signaling Technology (CST) via Beijing Chengzhi Kewei Biotechnology Co., Ltd. (China).

    Fluorescence:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Microscopy:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Staining:

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark. .. Finally, the fluorescence images of the stained cells were obtained using a confocal laser scanning microscope (Leica TCS SP8, Leica Microsystems, Wetzlar, Germany) and analyzed with ImageJ software.



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    Millipore fluoroshield with dapi
    Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with <t>DAPI</t> nuclear counterstain; scale bars = 1 mm and 100 µm (inset).
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    Image Search Results


    Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

    Journal: Frontiers in Dental Medicine

    Article Title: Salivary gland transcriptomic analysis and immunophenotyping in the IL-14α transgenic mouse model of Sjögren's disease

    doi: 10.3389/fdmed.2025.1612522

    Figure Lengend Snippet: Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

    Article Snippet: Slides were then washed in PBS and coverslips mounted using Fluoroshield with DAPI (MilliporeSigma).

    Techniques: Staining, Immunofluorescence, Marker