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fluorogenic probe  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fluorogenic probe
    Fluorogenic Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+probe/2'%2C7'-Dichlorofluorescein/pm42035742-145-48-53
    Average 99 stars, based on 1 article reviews
    fluorogenic probe - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    H2O2 Assay:

    Article Title: Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition
    Article Snippet: .. Intracellular ROS levels were measured using a ROS-Glo H2O2 assay (Promega) and CellROX Green reagent as a fluorogenic probe (Invitrogen). .. Luminescence was measured using an EnSpire multimode plate reader (PerkinElmer).

    Isolation:

    Article Title: Thioredoxin overexpression in mitochondria showed minimum effects on aging and age-related diseases in male C57BL/6 mice
    Article Snippet: .. H2O2 release from isolated mitochondria obtained from skeletal muscle was measured using the fluorogenic probe, Amplex Red (Molecular Probes, Eugene, OR) as previously described [ ]. ..

    Membrane:

    Article Title: The sRNA-encoded peptide TimP rewires the Escherichia coli envelope via LamB to sensitize bacteria to polymyxins.
    Article Snippet: The rise of multidrug-resistant (MDR) Escherichia coli has marginalized last-line polymyxins (PMs) owing to evolving resistance and narrow therapeutic windows.. Revitalizing these agents requires the identification of novel regulatory circuits governing the integrity of the gram-negative envelope.. In this study, we identified a stress-responsive peptide TimP and demonstrated that its intracellular overexpression (TimP), a synthetic variant (FT-Pep), and biogenic TimP-derived extracellular vesicles (TimP EVs) exhibited equivalent functionality in sensitizing E. coli to PMs, consistently inducing PM-mediated phenotypes.

    Binding Assay:

    Article Title: The sRNA-encoded peptide TimP rewires the Escherichia coli envelope via LamB to sensitize bacteria to polymyxins.
    Article Snippet: The rise of multidrug-resistant (MDR) Escherichia coli has marginalized last-line polymyxins (PMs) owing to evolving resistance and narrow therapeutic windows.. Revitalizing these agents requires the identification of novel regulatory circuits governing the integrity of the gram-negative envelope.. In this study, we identified a stress-responsive peptide TimP and demonstrated that its intracellular overexpression (TimP), a synthetic variant (FT-Pep), and biogenic TimP-derived extracellular vesicles (TimP EVs) exhibited equivalent functionality in sensitizing E. coli to PMs, consistently inducing PM-mediated phenotypes.

    Permeability:

    Article Title: The sRNA-encoded peptide TimP rewires the Escherichia coli envelope via LamB to sensitize bacteria to polymyxins.
    Article Snippet: The rise of multidrug-resistant (MDR) Escherichia coli has marginalized last-line polymyxins (PMs) owing to evolving resistance and narrow therapeutic windows.. Revitalizing these agents requires the identification of novel regulatory circuits governing the integrity of the gram-negative envelope.. In this study, we identified a stress-responsive peptide TimP and demonstrated that its intracellular overexpression (TimP), a synthetic variant (FT-Pep), and biogenic TimP-derived extracellular vesicles (TimP EVs) exhibited equivalent functionality in sensitizing E. coli to PMs, consistently inducing PM-mediated phenotypes.

    Concentration Assay:

    Article Title: Polydopamine Nanoparticles as Mimicking RPE Melanin for the Protection of Retinal Cells Against Blue Light‐Induced Phototoxicity
    Article Snippet: To assess ROS levels, DCF fluorescence intensity was measured using a spectrophotometer (Molecular Devices Spectramax M5) with excitation and emission wavelengths of 485 and 530 nm, respectively. .. To confirm the intracellular ROS levels in live pRPE cells exposed to blue light‐induced phototoxicity, 5 μ m (final concentration) of a fluorogenic probe (MitoSOX, Invitrogen) was added to each well after incubation with PDA. ..

    Incubation:

    Article Title: Polydopamine Nanoparticles as Mimicking RPE Melanin for the Protection of Retinal Cells Against Blue Light‐Induced Phototoxicity
    Article Snippet: To assess ROS levels, DCF fluorescence intensity was measured using a spectrophotometer (Molecular Devices Spectramax M5) with excitation and emission wavelengths of 485 and 530 nm, respectively. .. To confirm the intracellular ROS levels in live pRPE cells exposed to blue light‐induced phototoxicity, 5 μ m (final concentration) of a fluorogenic probe (MitoSOX, Invitrogen) was added to each well after incubation with PDA. ..



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    Kaneka Corp fluorogenic probe
    (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
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    Image Search Results


    (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

    Journal: medRxiv

    Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry

    doi: 10.64898/2026.04.28.26351941

    Figure Lengend Snippet: (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

    Article Snippet: For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT).

    Techniques: Staining, Virus, Infection