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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
Memglow 488, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/memglow 488/product/Cytoskeleton Inc
Average 95 stars, based on 1 article reviews
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
Memglow488, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/memglow488/product/Cytoskeleton Inc
Average 95 stars, based on 1 article reviews
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
Memglowtm 640, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/memglowtm 640/product/Cytoskeleton Inc
Average 95 stars, based on 1 article reviews
memglowtm 640 - by Bioz Stars, 2026-06
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
Memglowtm 488, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/memglowtm 488/product/Cytoskeleton Inc
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
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(A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

Journal: medRxiv

Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry

doi: 10.64898/2026.04.28.26351941

Figure Lengend Snippet: (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

Article Snippet: For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT).

Techniques: Staining, Virus, Infection