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fluorescence microscopy with dapi  (Vector Laboratories)


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    Vector Laboratories fluorescence microscopy with dapi
    Fluorescence Microscopy With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+microscopy+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm36373226-137-8-12
    Average 98 stars, based on 21970 article reviews
    fluorescence microscopy with dapi - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Corticostriatal Suppression of Appetitive Pavlovian Conditioned Responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (catalog #H-1200, Vector Laboratories) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4 lens for cell bodies and a 20 lens for neuron terminals.

    Article Title: Optogenetic stimulation of infralimbic cortex projections to the paraventricular thalamus attenuates context-induced renewal.
    Article Snippet: A Commentary on this Article is available here: https://doi.org/10.1111/ejn.15924.. Abstract Contexts associated with prior reinforcement can renew extinguished conditioned responding.. The prelimbic (PL) and infralimbic (IL) cortices are thought to mediate the expression and suppression of conditioned responding, respectively.

    Article Title: Silencing of Fas, but Not Caspase-8, in Lung Epithelial Cells Ameliorates Pulmonary Apoptosis, Inflammation, and Neutrophil Influx after Hemorrhagic Shock and Sepsis
    Article Snippet: Slides were then incubated with a streptavidin Alexa Fluor 594 conjugate (Molecular Probes) for 45 minutes, rinsed, and compared with negative-control slides (without primary). .. Slides were then coverslipped with a mounting medium for fluorescence microscopy with DAPI (Vector Laboratories), a DNA stain. ..

    Article Title: Corticostriatal suppression of appetitive Pavlovian conditioned responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (Vector labs, H-1200) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4x lens for cell bodies and a 20x lens for neuron terminals.

    Article Title: ImmunoPET for assessing the differential uptake of a CD146-specific monoclonal antibody in lung cancer
    Article Snippet: .. A coverslip was applied to each slide using Vectashield mounting medium for fluorescence microscopy with DAPI (4′,6-diamidino-2-phenylindole) (Vector Laboratories, Burlingame, CA, USA). .. All images were acquired using an A1R confocal microscope (Nikon).

    Staining:

    Article Title: Corticostriatal Suppression of Appetitive Pavlovian Conditioned Responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (catalog #H-1200, Vector Laboratories) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4 lens for cell bodies and a 20 lens for neuron terminals.

    Article Title: Silencing of Fas, but Not Caspase-8, in Lung Epithelial Cells Ameliorates Pulmonary Apoptosis, Inflammation, and Neutrophil Influx after Hemorrhagic Shock and Sepsis
    Article Snippet: Slides were then incubated with a streptavidin Alexa Fluor 594 conjugate (Molecular Probes) for 45 minutes, rinsed, and compared with negative-control slides (without primary). .. Slides were then coverslipped with a mounting medium for fluorescence microscopy with DAPI (Vector Laboratories), a DNA stain. ..

    Article Title: Corticostriatal suppression of appetitive Pavlovian conditioned responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (Vector labs, H-1200) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4x lens for cell bodies and a 20x lens for neuron terminals.

    Fluorescence:

    Article Title: Corticostriatal Suppression of Appetitive Pavlovian Conditioned Responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (catalog #H-1200, Vector Laboratories) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4 lens for cell bodies and a 20 lens for neuron terminals.

    Article Title: Optogenetic stimulation of infralimbic cortex projections to the paraventricular thalamus attenuates context-induced renewal.
    Article Snippet: A Commentary on this Article is available here: https://doi.org/10.1111/ejn.15924.. Abstract Contexts associated with prior reinforcement can renew extinguished conditioned responding.. The prelimbic (PL) and infralimbic (IL) cortices are thought to mediate the expression and suppression of conditioned responding, respectively.

    Article Title: Silencing of Fas, but Not Caspase-8, in Lung Epithelial Cells Ameliorates Pulmonary Apoptosis, Inflammation, and Neutrophil Influx after Hemorrhagic Shock and Sepsis
    Article Snippet: Slides were then incubated with a streptavidin Alexa Fluor 594 conjugate (Molecular Probes) for 45 minutes, rinsed, and compared with negative-control slides (without primary). .. Slides were then coverslipped with a mounting medium for fluorescence microscopy with DAPI (Vector Laboratories), a DNA stain. ..

    Article Title: Corticostriatal suppression of appetitive Pavlovian conditioned responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (Vector labs, H-1200) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4x lens for cell bodies and a 20x lens for neuron terminals.

    Article Title: ImmunoPET for assessing the differential uptake of a CD146-specific monoclonal antibody in lung cancer
    Article Snippet: .. A coverslip was applied to each slide using Vectashield mounting medium for fluorescence microscopy with DAPI (4′,6-diamidino-2-phenylindole) (Vector Laboratories, Burlingame, CA, USA). .. All images were acquired using an A1R confocal microscope (Nikon).

    Expressing:

    Article Title: Corticostriatal Suppression of Appetitive Pavlovian Conditioned Responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (catalog #H-1200, Vector Laboratories) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4 lens for cell bodies and a 20 lens for neuron terminals.

    Article Title: Corticostriatal suppression of appetitive Pavlovian conditioned responding
    Article Snippet: .. Brain sections were mounted onto microscope slides and processed for Nissl staining or fluorescence microscopy with DAPI (Vector labs, H-1200) to verify optical fiber placement and transgene expression. .. Images of transgene expression were captured through an epifluorescence microscope (Nikon Eclipse TiE) using a 4x lens for cell bodies and a 20x lens for neuron terminals.



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    Matisse Pharmaceuticals fluorescent microscopy images based on dapi nuclear staining
    Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
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    Image Search Results


    Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and DAPI. The tissue was first scanned using a fluorescent microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image

    Journal: BMC Biology

    Article Title: MATISSE: a method for improved single cell segmentation in imaging mass cytometry

    doi: 10.1186/s12915-021-01043-y

    Figure Lengend Snippet: Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and DAPI. The tissue was first scanned using a fluorescent microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image

    Article Snippet: We observed that incorporating fluorescent microscopy images based on DAPI nuclear staining into MATISSE workflow resulted in superior visual and signal intensity-based separation of nuclei in dense areas (Fig. b, Additional file : Fig S1B).

    Techniques: Fluorescence, Microscopy, Multiplex Assay, Imaging, Staining, Produced, Labeling, Membrane