fluorescence microscopy (Beyotime)
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Beyotime
fluorescence microscopy
Fluorescence Microscopy, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pm40471885-99-2-19
Average 99 stars, based on 32308 article reviews
Fluorescence Microscopy, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pm40471885-99-2-19
Average 99 stars, based on 32308 article reviews
fluorescence microscopy - by Bioz Stars,
2026-09
99/100 stars
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Staining:Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer Article Snippet: .. After treatment, all cells were washed twice with PBS, and fixed with 4% paraformaldehyde for 15 min. After permeabilization with 0.5% Triton X-100 for 10 min, cells were stained with Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential Article Snippet: In short, the cells were washed twice with PBS, fixed in 4 % paraformaldehyde (PFA, Solarbio, China) for 15 min, and then washed with PBS again. .. The cells were permeabilized with 0.2 %Triton X-100 solution for 15 min, followed by incubation with FITC-phalloidin dye (Yeasen, China) in the dark for 30 min, and then stained with 4’, Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration Article Snippet: The pelleted EVs were pre-labeled with 10 μM PHK26 dye (Beyotime, C2017S) and then co-cultured with NPCs for 12 h, respectively. .. After fixation with 4% paraformaldehyde for 15 min, the Actin-tracker was used for cytoskeleton staining (Beyotime, C2201S), and the nucleus was stained with Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with Incubation:Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential Article Snippet: In short, the cells were washed twice with PBS, fixed in 4 % paraformaldehyde (PFA, Solarbio, China) for 15 min, and then washed with PBS again. .. The cells were permeabilized with 0.2 %Triton X-100 solution for 15 min, followed by incubation with FITC-phalloidin dye (Yeasen, China) in the dark for 30 min, and then stained with 4’, Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair Article Snippet: .. The OBNC microspheres were added to the working solution according to the manufacturer's instructions, incubated for 30 min in the dark, and an anti-fluorescence quencher containing TUNEL Assay:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Microscopy:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and Apoptosis Assay:Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Polymer:Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Real-time Polymerase Chain Reaction:Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Polymerase Chain Reaction:Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Isolation:Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Purification:Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), Laser-Scanning Microscopy:Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury Article Snippet: Cells were then incubated overnight at 4 °C with rabbit polyclonal antibodies against CD86 or CD206 (1:200, Proteintech), followed by 1 h incubation with Alexa Fluor 488-conjugated or CoraLite Plus 594-conjugated goat anti-rabbit IgG secondary antibodies (1:200, Proteintech). .. Nuclei were counterstained with |
![Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4487/pmc08114487/pmc08114487__12915_2021_1043_Fig1_HTML.jpg)