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falcon lifetime imaging module  (Danaher Inc)


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    Structured Review

    Danaher Inc falcon lifetime imaging module
    Falcon Lifetime Imaging Module, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flim+module/STELLARIS+8+FALCON+FLIM+Microscopes/bio_rxiv__64898__2026__10__09__757842-241-17-21
    Average 96 stars, based on 303 article reviews
    falcon lifetime imaging module - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Chemical sensing and imaging using fluorophore-conjugated cellulose nanocrystals
    Article Snippet: Here, we report the use of highly fluorescent zinc phthalocyanine-conjugated cellulose nanocrystals (ZnPc@CNC) for chemical sensing and imaging applications.. Cellulose nanocrystals (CNCs) are crystalline nanorods synthesized through the acid hydrolysis of cellulosic resources like wood pulp, cotton fibers, carded hemp, etc. and lab-synthesized octacarboxylated zinc phthalocyanine molecules are conjugated to these CNCs forming a brightly fluorescent-conjugated molecular aggregate (ZnPc@CNC), which was then used in both liquid suspensions and solution-processed thin films.. ZnPc@CNC conjugates showed reproducible and reliable photoluminescence (PL) quenching behavior when exposed to terephthalic acid (TA) of concentration 0.2 mM to 0.8 mM.

    Article Title: Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner
    Article Snippet: Live HEK293T cells expressing various SNAP-tagged membrane proteins were stained with 5μM or less of SNAP-Surface 488 substrate, a modified ATTO 488 molecule, and with either membrane dyes, PlasMem Bright Red (Dojindo) or MemGlow 590 (Cytoskelton). .. Doubly stained cells were imaged by Leica SP8 equipped with FLIM module, and the lifetime of fluorescence of donor ATTO 488 was measured and imaged in the FLIM mode. ..

    Imaging:

    Article Title: Chemical sensing and imaging using fluorophore-conjugated cellulose nanocrystals
    Article Snippet: Here, we report the use of highly fluorescent zinc phthalocyanine-conjugated cellulose nanocrystals (ZnPc@CNC) for chemical sensing and imaging applications.. Cellulose nanocrystals (CNCs) are crystalline nanorods synthesized through the acid hydrolysis of cellulosic resources like wood pulp, cotton fibers, carded hemp, etc. and lab-synthesized octacarboxylated zinc phthalocyanine molecules are conjugated to these CNCs forming a brightly fluorescent-conjugated molecular aggregate (ZnPc@CNC), which was then used in both liquid suspensions and solution-processed thin films.. ZnPc@CNC conjugates showed reproducible and reliable photoluminescence (PL) quenching behavior when exposed to terephthalic acid (TA) of concentration 0.2 mM to 0.8 mM.

    Article Title: Lamin B Receptor Upregulation in Metastatic Melanoma Causes Cholesterol-Mediated Nuclear Envelope Fragility
    Article Snippet: .. Two-photon imaging of whole-mounted 1205Lu melanoma tumors that had been resected from mice was performed using an upright Leica SP8 DIVE (Deep In Vivo Explorer) 2-photon system using a Leica HC Fluotar L VISIR 25x/0.95 Water immersion objective lens (working distance 2.4 mm) and equipped with a FALCON FLIM module (Leica Microsystems, Mannheim, Germany). .. For 2-photon excitation, an infra-red laser, InSight X3 (Spectra-Physics), dual-beam with one fixed 1045-nm line and one tunable line from 680 to 1300 nm was used.

    Microscopy:

    Article Title: Chemical sensing and imaging using fluorophore-conjugated cellulose nanocrystals
    Article Snippet: Here, we report the use of highly fluorescent zinc phthalocyanine-conjugated cellulose nanocrystals (ZnPc@CNC) for chemical sensing and imaging applications.. Cellulose nanocrystals (CNCs) are crystalline nanorods synthesized through the acid hydrolysis of cellulosic resources like wood pulp, cotton fibers, carded hemp, etc. and lab-synthesized octacarboxylated zinc phthalocyanine molecules are conjugated to these CNCs forming a brightly fluorescent-conjugated molecular aggregate (ZnPc@CNC), which was then used in both liquid suspensions and solution-processed thin films.. ZnPc@CNC conjugates showed reproducible and reliable photoluminescence (PL) quenching behavior when exposed to terephthalic acid (TA) of concentration 0.2 mM to 0.8 mM.

    In Vivo:

    Article Title: Lamin B Receptor Upregulation in Metastatic Melanoma Causes Cholesterol-Mediated Nuclear Envelope Fragility
    Article Snippet: .. Two-photon imaging of whole-mounted 1205Lu melanoma tumors that had been resected from mice was performed using an upright Leica SP8 DIVE (Deep In Vivo Explorer) 2-photon system using a Leica HC Fluotar L VISIR 25x/0.95 Water immersion objective lens (working distance 2.4 mm) and equipped with a FALCON FLIM module (Leica Microsystems, Mannheim, Germany). .. For 2-photon excitation, an infra-red laser, InSight X3 (Spectra-Physics), dual-beam with one fixed 1045-nm line and one tunable line from 680 to 1300 nm was used.

    Staining:

    Article Title: Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner
    Article Snippet: Live HEK293T cells expressing various SNAP-tagged membrane proteins were stained with 5μM or less of SNAP-Surface 488 substrate, a modified ATTO 488 molecule, and with either membrane dyes, PlasMem Bright Red (Dojindo) or MemGlow 590 (Cytoskelton). .. Doubly stained cells were imaged by Leica SP8 equipped with FLIM module, and the lifetime of fluorescence of donor ATTO 488 was measured and imaged in the FLIM mode. ..



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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Image Search Results


    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Viscosity, Control

    Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Control