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a1 spectral detector confocal with flim module  (Nikon)


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    Structured Review

    Nikon a1 spectral detector confocal with flim module
    A1 Spectral Detector Confocal With Flim Module, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flim+module/pm39705926-66-19-13
    Average 90 stars, based on 1 article reviews
    a1 spectral detector confocal with flim module - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Confocal Microscopy:

    Article Title: Direct Cytosolic Delivery of Citraconylated Proteins.
    Article Snippet: Alamar Blue (Invitrogen) viability studies were performed according to the manufacturer’s instructions, using Molecular Devices Spectramax M2 and SoftMax Pro 7 software. .. Pharmaceutics 2023, 15, 218 3 of 10 Confocal microscopy Confocal microscopy imaging experiments were performed using a Nikon TiE stand with an A1 Spectral Detector Confocal and a FLIM/FCS Module. .. For confocal studies, 2.0 × 105 cells were seeded in a 35 mm imaging dish (Mattek) a day before the imaging experiment.

    Imaging:

    Article Title: Direct Cytosolic Delivery of Citraconylated Proteins.
    Article Snippet: Alamar Blue (Invitrogen) viability studies were performed according to the manufacturer’s instructions, using Molecular Devices Spectramax M2 and SoftMax Pro 7 software. .. Pharmaceutics 2023, 15, 218 3 of 10 Confocal microscopy Confocal microscopy imaging experiments were performed using a Nikon TiE stand with an A1 Spectral Detector Confocal and a FLIM/FCS Module. .. For confocal studies, 2.0 × 105 cells were seeded in a 35 mm imaging dish (Mattek) a day before the imaging experiment.

    Article Title: Surface functionalization of poly(dimethylsiloxane) substrates facilitates culture of pre-implantation mouse embryos by blocking non-selective adsorption.
    Article Snippet: After three times of washes, embryos were incubated with suitable secondary antibodies (1 : 500 dilution, royalsocietypublishing.org/journal/rsif J.R.Soc.Interface 19:20210929 3 D ow nl oa de d fr om h ttp s: //r oy al so ci et yp ub lis hi ng .o rg / o n 18 F eb ru ar y 20 24 Alexa Fluor, Thermo Fisher Scientific) for 1 h at room temperature in dark. .. After two washes, DNA was stained with 4,6- diamidino-2-phenylindole (DAPI) before the final wash and mounting for imaging under a Nikon A1 Spectral Detector Confocal with FLIM Module. ..

    other:

    Article Title: Direct Cytosolic Delivery of Citraconylated Proteins
    Article Snippet: Confocal microscopy imaging experiments were performed using a Nikon TiE stand with an A1 Spectral Detector Confocal and a FLIM/FCS Module.

    Article Title: Distinct taxonomic and phylogenetic patterns of plant communities on acid and limestone soils in subtropical and tropical China
    Article Snippet: Construction of polymer−protein nanoassemblies is a challenge as reactions between macromolecules, especially those involving proteins, are inherently inefficient due to the sparse reactive functional groups and low concentration requirements.. We address this challenge using an ultrafast and reversible click reaction, which forms the basis for a covalent self-assembly strategy between side-chain functionalized polymers and surface-modified proteins.. The linkers in the assembly have been programmed to release the incarcerated proteins in its native form, only when subjected to the presence of a specific trigger.

    Article Title: Development exposures to perfluorooctanesulfonic acid (PFOS) impact embryonic nutrition, pancreatic morphology, and adiposity in the zebrafish, Danio rerio
    Article Snippet: Preserved fish were stored in VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Burlingame, CA) until imaging.

    Article Title: Dual antimicrobial-loaded biodegradable nanoemulsions for synergistic treatment of wound biofilms.
    Article Snippet: A Nikon A1 spectral detector confocal with FLIM module was used to monitor the penetration profile of the nanoemulsions.

    Staining:

    Article Title: Surface functionalization of poly(dimethylsiloxane) substrates facilitates culture of pre-implantation mouse embryos by blocking non-selective adsorption.
    Article Snippet: After three times of washes, embryos were incubated with suitable secondary antibodies (1 : 500 dilution, royalsocietypublishing.org/journal/rsif J.R.Soc.Interface 19:20210929 3 D ow nl oa de d fr om h ttp s: //r oy al so ci et yp ub lis hi ng .o rg / o n 18 F eb ru ar y 20 24 Alexa Fluor, Thermo Fisher Scientific) for 1 h at room temperature in dark. .. After two washes, DNA was stained with 4,6- diamidino-2-phenylindole (DAPI) before the final wash and mounting for imaging under a Nikon A1 Spectral Detector Confocal with FLIM Module. ..

    Polymer:

    Article Title: Cationic conjugated polymers with tunable hydrophobicity for efficient treatment of multidrug-resistant wound biofilm infections.
    Article Snippet: Biofilm-associated infections arising from antibiotic-resistant bacteria pose a critical challenge to global health.. We report the generation of a library of cationic conjugated poly(phenylene ethynylene) (PPE) polymers featuring trimethylammonium terminated sidechains with tunable hydrophobicity.. Screening of the library identified an amphiphilic polymer with a C11 hydrophobic spacer as the polymer with the highest antimicrobial efficacy against biofilms in the dark with excellent selectivity.

    Confocal:

    Article Title: Visualization of Polymer Dynamics in Cellulose Nanocrystal Matrices Using Fluorescence Lifetime Measurements.
    Article Snippet: Polymer nanocomposites containing self-assembled cellulose nanocrystals (CNCs) are ideal for advanced applications requiring both strength and toughness as the helicoidal structure of the CNCs deflects crack propagation and the polymer matrix dissipates impact energy.. However, any adsorbed water layer surrounding the CNCs may compromise the interfacial adhesion between the polymer matrix and the CNCs, thus impacting stress transfer at that interface.. Therefore, it is critical to study the role of water at the interface in connecting the polymer dynamics and the resulting mechanical performance of the nanocomposite.

    Fluorescence:

    Article Title: Visualization of Polymer Dynamics in Cellulose Nanocrystal Matrices Using Fluorescence Lifetime Measurements.
    Article Snippet: Polymer nanocomposites containing self-assembled cellulose nanocrystals (CNCs) are ideal for advanced applications requiring both strength and toughness as the helicoidal structure of the CNCs deflects crack propagation and the polymer matrix dissipates impact energy.. However, any adsorbed water layer surrounding the CNCs may compromise the interfacial adhesion between the polymer matrix and the CNCs, thus impacting stress transfer at that interface.. Therefore, it is critical to study the role of water at the interface in connecting the polymer dynamics and the resulting mechanical performance of the nanocomposite.



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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Image Search Results


    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Viscosity, Control

    Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Control