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flagellin tlrl epstfla  (InvivoGen)


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    InvivoGen flagellin tlrl epstfla
    Flagellin Tlrl Epstfla, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flagellin/FLA-ST+Ultrapure/pm42217341-110-6-11
    Average 96 stars, based on 278 article reviews
    flagellin tlrl epstfla - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Sirt3 Genetically Engineered Apoptotic Bodies Alleviate Skeletal Aging by Limiting Aggravated NLRP3 Inflammasome Activation of Senescent Macrophages
    Article Snippet: .. For the activation of NLRC4 or AIM2 inflammasome, LPS‐primed BMDMs were stimulated with flagellin (1μg/mL; 6h; tlrl‐pafla; InvivoGen) or transfected dsDNA (1μg; 6h; tlrl‐patc; InvivoGen) as indicated. ..

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: For NLRP3 inflammasome activation, cells were primed for 4 h with 500 ng/mL ultrapure LPS from Escherichia coli (0111:B4) (InvivoGen, tlrl-3pelps) and then stimulated for 6 h with 1 μM nigericin (InvivoGen, tlrl-nig). .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). ..

    Transfection:

    Article Title: Sirt3 Genetically Engineered Apoptotic Bodies Alleviate Skeletal Aging by Limiting Aggravated NLRP3 Inflammasome Activation of Senescent Macrophages
    Article Snippet: .. For the activation of NLRC4 or AIM2 inflammasome, LPS‐primed BMDMs were stimulated with flagellin (1μg/mL; 6h; tlrl‐pafla; InvivoGen) or transfected dsDNA (1μg; 6h; tlrl‐patc; InvivoGen) as indicated. ..

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: For NLRP3 inflammasome activation, cells were primed for 4 h with 500 ng/mL ultrapure LPS from Escherichia coli (0111:B4) (InvivoGen, tlrl-3pelps) and then stimulated for 6 h with 1 μM nigericin (InvivoGen, tlrl-nig). .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). ..

    Incubation:

    Article Title: Hijacking of inflammasome responses by the complement system during Pseudomonas aeruginosa – Aspergillus fumigatus sur-infection
    Article Snippet: .. For priming experiments, BMDMs were incubated with 100 ng/mL LPS (PA10 L9143, Sigma-Aldrich), 1 μg/mL flagellin (tlrl-pafla, Invivogen), or 100 ng/mL IFN-γ (P01579, Invivogen) for 4 hours before A. fumigatus infection. ..

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: For NLRP3 inflammasome activation, cells were primed for 4 h with 500 ng/mL ultrapure LPS from Escherichia coli (0111:B4) (InvivoGen, tlrl-3pelps) and then stimulated for 6 h with 1 μM nigericin (InvivoGen, tlrl-nig). .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). ..

    Infection:

    Article Title: Hijacking of inflammasome responses by the complement system during Pseudomonas aeruginosa – Aspergillus fumigatus sur-infection
    Article Snippet: .. For priming experiments, BMDMs were incubated with 100 ng/mL LPS (PA10 L9143, Sigma-Aldrich), 1 μg/mL flagellin (tlrl-pafla, Invivogen), or 100 ng/mL IFN-γ (P01579, Invivogen) for 4 hours before A. fumigatus infection. ..

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: For NLRP3 inflammasome activation, cells were primed for 4 h with 500 ng/mL ultrapure LPS from Escherichia coli (0111:B4) (InvivoGen, tlrl-3pelps) and then stimulated for 6 h with 1 μM nigericin (InvivoGen, tlrl-nig). .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). ..

    Expressing:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: For NLRP3 inflammasome activation, cells were primed for 4 h with 500 ng/mL ultrapure LPS from Escherichia coli (0111:B4) (InvivoGen, tlrl-3pelps) and then stimulated for 6 h with 1 μM nigericin (InvivoGen, tlrl-nig). .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). ..

    Plasmid Preparation:

    Article Title: Herpes simplex virus 1 harboring poly(T) DNA sequences as a key ligand for AIM2 inflammasome activation and host defense.
    Article Snippet: For NLRP3 inflammasome activation, cells were primed for 4 h with 500 ng/mL ultrapure LPS from Escherichia coli (0111:B4) (InvivoGen, tlrl-3pelps) and then stimulated for 6 h with 1 μM nigericin (InvivoGen, tlrl-nig). .. For ligandmediated AIM2 inflammasome activation, 1 ng of poly(dA:dT) (InvivoGen, tlrl-patn), poly(dG:dC) (InvivoGen, tlrl-pgcn), or poly(T) DNA of 10-, 30-, or 90-mer length was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. For NLRC4 inflammasome activation, 10 ng of flagellin (Invivogen, tlrl-epstfla5) was transfected into cells using DOTAP (Roche, 11202375001) according to the manufacturer’s instructions, followed by incubation for 6 h. In the context of VP22, F strain VP22 vector (pLV[Exp]-EGFP:T2A:Puro-EF1A>VP22) was purchased (Vector Builder, VB2209201383rfw) and BMDMs were transfected with the VP22 expression plasmid 12 h prior to viral infection using DOTAP (Roche, 11202375001). ..

    Molecular Weight:

    Article Title: Folate receptor beta drives NLRP3 inflammasome activation and pyroptosis in macrophages independent of folate binding.
    Article Snippet: Cleaved GSDMD and pro-GSDMD antibodies for WB were purchased from Cell Signaling Technologies (Danvers, MA, USA). .. Ultrapure LPS from Escherichia coli 0111:B4 (LPS; TLR4 agonist), FSL-1 (synthetic diacylated lipoprotein; TLR 2/6 agonist), thiazoloquinoline (CL075; TLR 7/8 agonist), synthetic triacylated lipopeptide (PAM3CSK4; TLR 1/2 agonist), lipoteichoic acid (LTA) from Staphylococcus aureus (TLR 2 agonist), Poly(I:C) (low molecular weight; TLR3 agonist), flagellin from Pseudomonas aeruginosa (TLR 5 agonist), imiquimod (R837; TLR7 agonist), and nigericin sodium salt were purchased from InvivoGen (San Diego, CA, USA). .. Human FcR blocking reagent, human CD14 microbeads, LS/large cell columns, and Dead Cell Removal kits were purchased from Miltenyi (San Diego, CA, USA).



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    Image Search Results


    TMH does not affect other inflammasome or inflammatory pathways. (A,B) LPS-primed J774A.1 cells were treated with TMH for 2 h, followed by stimulation with either dsDNA (1 μg/mL) (A) or flagellin (B) transfected using Lipofectamine 2000 for 3 h. (C) Following TMH treatment (2 h), nuclear and cytosolic fractions of LPS-primed J774A.1 cells were separated and assessed for NF-κB p65 translocation by immunoblotting. (D) HEK293FT cells transfected with the pGL4.32 luciferase reporter vector were treated with TNF-α (20 ng/mL) for 5 h, with or without TMH pre-treatment (2 h). Luciferase activity was measured using the Bright-Glo™ luciferase assay system (Promega). Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test n.s., not significant.

    Journal: Frontiers in Pharmacology

    Article Title: Thymelaea hirsuta (L.) Endl . extract attenuates NLRP3 inflammasome activation via modulation of ATPase activity

    doi: 10.3389/fphar.2026.1781860

    Figure Lengend Snippet: TMH does not affect other inflammasome or inflammatory pathways. (A,B) LPS-primed J774A.1 cells were treated with TMH for 2 h, followed by stimulation with either dsDNA (1 μg/mL) (A) or flagellin (B) transfected using Lipofectamine 2000 for 3 h. (C) Following TMH treatment (2 h), nuclear and cytosolic fractions of LPS-primed J774A.1 cells were separated and assessed for NF-κB p65 translocation by immunoblotting. (D) HEK293FT cells transfected with the pGL4.32 luciferase reporter vector were treated with TNF-α (20 ng/mL) for 5 h, with or without TMH pre-treatment (2 h). Luciferase activity was measured using the Bright-Glo™ luciferase assay system (Promega). Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test n.s., not significant.

    Article Snippet: For inflammasome stimulation, ultrapure LPS (tlrl-ppglps), nigericin (tlrl-nig), adenosine triphosphate (ATP; tlrl-atpl), and flagellin (FLA-ST; tlrl-epstfla) were obtained from InvivoGen (United States).

    Techniques: Transfection, Translocation Assay, Western Blot, Luciferase, Plasmid Preparation, Activity Assay