rabbit anti fkbp5  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Unveiling potential diagnostic biomarkers for rheumatoid arthritis through integrated gene expression analysis

doi: 10.3389/fimmu.2026.1645257

Figure Lengend Snippet: Development and validation of the RA diagnostic columnar line graph model. (A) Columnar line graphs used to predict RA incidence. (B) ROC curve analysis to evaluate the clinical utility of the columnar line graph model. (C) DCA curve analysis to assess the clinical utility of the columnar line graph model. (D) Box plot illustrating the expression levels of GABARAPL1, FKBP5, MREG, PCDH9, and SLAMF8.

Article Snippet: Subsequently, they were incubated with the primary antibodies against PCDH9 (Abcam, ab233710), GABARAPL1 (Abcam, ab109364), FKBP5 (Proteintech, 14155-1-AP), SLAMF8 (Novus Biologicals, AF1907), and GAPDH (Proteintech, 10494-1-AP) for an overnight period at 4°C.

Techniques: Biomarker Discovery, Diagnostic Assay, Expressing

Journal: Frontiers in Immunology

Article Title: Unveiling potential diagnostic biomarkers for rheumatoid arthritis through integrated gene expression analysis

doi: 10.3389/fimmu.2026.1645257

Figure Lengend Snippet: Analysis of hub gene expression in synovial tissues from RA patients. (A) Representative MRI scans illustrating synovial thickening. (B) H&E staining of synovial tissue in trauma control (TC) and RA samples (n = 3). (C) Comparative analysis of mRNA expression levels for GABARAPL1, FKBP5, MREG, PCDH9, and SLAMF8 in TC and RA samples (n = 9). (D) Quantitative assessment of protein concentrations for GABARAPL1, FKBP5, PCDH9, and SLAMF8 in TC and RA samples (n = 3, the samples derive from the same experiment and that gels/blots were processed in parallel. original blots/gels are presented in Supplementary Figure 1 ). (E) IF staining results showed protein expression of GABARAPL1, FKBP5, PCDH9, and SLAMF8 in TC and RA samples, visualized with DAPI (blue) and specific antibodies (green) (n = 3). The means ± SD are used to represent the data, while ns (not significant), * P < 0.05, ** P < 0.01, and * P < 0.001 indicate significance levels.

Article Snippet: Subsequently, they were incubated with the primary antibodies against PCDH9 (Abcam, ab233710), GABARAPL1 (Abcam, ab109364), FKBP5 (Proteintech, 14155-1-AP), SLAMF8 (Novus Biologicals, AF1907), and GAPDH (Proteintech, 10494-1-AP) for an overnight period at 4°C.

Techniques: Gene Expression, Staining, Control, Expressing

Journal: Frontiers in Immunology

Article Title: Unveiling potential diagnostic biomarkers for rheumatoid arthritis through integrated gene expression analysis

doi: 10.3389/fimmu.2026.1645257

Figure Lengend Snippet: Correlation between candidate gene expression and inflammatory indices in the RA cohort. Scatter plots show the associations between PCR-measured expression levels of the candidate genes (GABARAPL1, FKBP5, PCDH9, and SLAMF8) and serum inflammatory markers (ESR and CRP) within RA patients only (n = 9). Spearman’s rank correlation was used to estimate the correlation coefficient ( R ), with p values adjusted for multiple testing using the Benjamini–Hochberg false discovery rate (FDR).

Article Snippet: Subsequently, they were incubated with the primary antibodies against PCDH9 (Abcam, ab233710), GABARAPL1 (Abcam, ab109364), FKBP5 (Proteintech, 14155-1-AP), SLAMF8 (Novus Biologicals, AF1907), and GAPDH (Proteintech, 10494-1-AP) for an overnight period at 4°C.

Techniques: Gene Expression, Expressing