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fish prl elisa kit  (Cusabio)


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    Cusabio fish prl elisa kit
    Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of <t>prl</t> CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b <t>ELISA</t> of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001
    Fish Prl Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fish prl elisa kit/product/Cusabio
    Average 93 stars, based on 3 article reviews
    fish prl elisa kit - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Glis3 as a critical regulator of Pit1-lineages and renal functions"

    Article Title: Glis3 as a critical regulator of Pit1-lineages and renal functions

    Journal: Journal of Molecular Medicine (Berlin, Germany)

    doi: 10.1007/s00109-025-02611-3

    Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of prl CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b ELISA of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001
    Figure Legend Snippet: Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of prl CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b ELISA of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001

    Techniques Used: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Confocal Microscopy, MANN-WHITNEY, Fluorescence, Software



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    Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of <t>prl</t> CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b <t>ELISA</t> of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001
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    Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of <t>prl</t> CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b <t>ELISA</t> of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001
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    Image Search Results


    Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of prl CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b ELISA of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001

    Journal: Journal of Molecular Medicine (Berlin, Germany)

    Article Title: Glis3 as a critical regulator of Pit1-lineages and renal functions

    doi: 10.1007/s00109-025-02611-3

    Figure Lengend Snippet: Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of prl CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b ELISA of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001

    Article Snippet: Prolactin levels (ng/ml) were measured using a Fish PRL ELISA Kit (CusaBio, CSB-E12695Fh).

    Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Confocal Microscopy, MANN-WHITNEY, Fluorescence, Software