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fip1l1  (Proteintech)


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    Proteintech fip1l1
    Fip1l1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fip1l1/FIP1L1+Antibody/pm41507200-314-22-24
    Average 93 stars, based on 4 article reviews
    fip1l1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3′ end processing core factors
    Article Snippet: The antibodies used in western blotting, co-IP, and immunofluorescence are as follows: MYC-tag (Sigma, M4439), FLAG-tag (Sigma, F1804), SNRPA (Abcepta, AW5557), SNRPC (Abcepta, AW5526), SNRNP70 (Santa Cruz Biotechnology, sc-390899), SNRPD2 (Abcam, ab198296), CPSF6 (Novus, NBP1-85676), PABPN1 (Abclonal, A1735), FIP1L1(Novus, NBP1-85064), CPSF5 (Proteintech, 66335-1-Ig), CPSF7 (Proteintech, 55195-1-AP), GAPDH (Proteintech, 60004-1-Ig), IgG-rabbit (Cell Signaling Technology, 2729S), HRP-linked-mouse (Cell Signaling Technology, 7076S), HRP-linked-rabbit (Cell Signaling Technology, 7074S), Alexa Fluor TM 568 labeled-rabbit (Invitrogen, A-11011), Alexa Fluor TM 488 labeled-mouse (Invitrogen, A-11001).

    Activity Assay:

    Article Title: Therapeutic targeting of CPSF3-dependent transcriptional termination in ovarian cancer
    Article Snippet: .. The slides were then treated with 3% H2O2 in methanol for 10 min to quench endogenous peroxidase activity, blocked with goat serum and incubated with primary antibodies against CPSF1 (ab81552, Abcam), CPSF2 (#17739-1-AP, Proteintech), CPSF3 (ab72295, Abcam), WDR33 (sc-374466, Santa Cruz), CPSF4 (#15023-1-AP, Proteintech), and FIP1L1 (#12276-1-AP, Proteintech) at 4°C overnight, followed by incubation with horseradish peroxidaseconjugated secondary antibody for 1 h at room temperature (RT). .. Antigen visualization was performed using 3,3’-diaminobenzidine (DAB) chromogen (Vector Laboratories).

    Incubation:

    Article Title: Therapeutic targeting of CPSF3-dependent transcriptional termination in ovarian cancer
    Article Snippet: .. The slides were then treated with 3% H2O2 in methanol for 10 min to quench endogenous peroxidase activity, blocked with goat serum and incubated with primary antibodies against CPSF1 (ab81552, Abcam), CPSF2 (#17739-1-AP, Proteintech), CPSF3 (ab72295, Abcam), WDR33 (sc-374466, Santa Cruz), CPSF4 (#15023-1-AP, Proteintech), and FIP1L1 (#12276-1-AP, Proteintech) at 4°C overnight, followed by incubation with horseradish peroxidaseconjugated secondary antibody for 1 h at room temperature (RT). .. Antigen visualization was performed using 3,3’-diaminobenzidine (DAB) chromogen (Vector Laboratories).



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    (a) Western blot analysis of UPR mt -related protein expression in HEK293T cells transfected with control (Ctrl) or FIP1L1 siRNA, and treated with or without doxycycline (Dox; 30Cμg/mL) for 24 hours. (b) Lollipop plot showing the metabolic health-related clinical traits that have suggestive (-Log10(P value) > 4) or significant ((-Log 10 (P value) > 8) GWAS hits within/around the FIP1L1 in the UKBB and million veteran program (MVP). (c) Mendelian randomization analysis for the causal effect of the gene expression of FIP1L1 in human skeletal muscle on metabolic-related traits. Thresholds were represented by red line (BH adjusted P value < 0.05).

    Journal: bioRxiv

    Article Title: Genetic mapping of lifespan and mitochondrial stress response in C. elegans

    doi: 10.1101/2025.06.26.661693

    Figure Lengend Snippet: (a) Western blot analysis of UPR mt -related protein expression in HEK293T cells transfected with control (Ctrl) or FIP1L1 siRNA, and treated with or without doxycycline (Dox; 30Cμg/mL) for 24 hours. (b) Lollipop plot showing the metabolic health-related clinical traits that have suggestive (-Log10(P value) > 4) or significant ((-Log 10 (P value) > 8) GWAS hits within/around the FIP1L1 in the UKBB and million veteran program (MVP). (c) Mendelian randomization analysis for the causal effect of the gene expression of FIP1L1 in human skeletal muscle on metabolic-related traits. Thresholds were represented by red line (BH adjusted P value < 0.05).

    Article Snippet: For western blotting, the antibodies used were: P-EIF2α (Cat. 3597, CST, 1:800), Tubulin (Cat. T5168, Sigma, 1:2000), ASNS (Santa Cruz, Cat. sc-365809, 1:1000), EIF2α (Cat. 9722, CST, 1:1000), ATF4 (Cat. 11815, CST, 1:1000), FIP1L1 (C-10) (Santa Cruz, Cat. sc-398392, 1:500).

    Techniques: Western Blot, Expressing, Transfection, Control, Gene Expression

    ( a-b ) Manhattan plot showing the associated gene expression modules of Fip1l1 in mouse (a) and human muscles (b) . Data were retrieved from https://systems-genetics.org/gmad . The threshold is represented by the red dashed line (absolute gene-module association score ≥ 0.268). Terms above the threshold are identified as th significant associated terms. GO terms or gene modules are ranked by similarity.

    Journal: bioRxiv

    Article Title: Genetic mapping of lifespan and mitochondrial stress response in C. elegans

    doi: 10.1101/2025.06.26.661693

    Figure Lengend Snippet: ( a-b ) Manhattan plot showing the associated gene expression modules of Fip1l1 in mouse (a) and human muscles (b) . Data were retrieved from https://systems-genetics.org/gmad . The threshold is represented by the red dashed line (absolute gene-module association score ≥ 0.268). Terms above the threshold are identified as th significant associated terms. GO terms or gene modules are ranked by similarity.

    Article Snippet: For western blotting, the antibodies used were: P-EIF2α (Cat. 3597, CST, 1:800), Tubulin (Cat. T5168, Sigma, 1:2000), ASNS (Santa Cruz, Cat. sc-365809, 1:1000), EIF2α (Cat. 9722, CST, 1:1000), ATF4 (Cat. 11815, CST, 1:1000), FIP1L1 (C-10) (Santa Cruz, Cat. sc-398392, 1:500).

    Techniques: Gene Expression, Muscles