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hkf human keloid fibroblasts cell line  (ATCC)


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    ATCC hkf human keloid fibroblasts cell line
    Glutamate reprogram keloid <t>fibroblasts</t> transcripts. (A) Volcano plot show DEGs in <t>HKF</t> cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .
    Hkf Human Keloid Fibroblasts Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hkf human keloid fibroblasts cell line/product/ATCC
    Average 95 stars, based on 69 article reviews
    hkf human keloid fibroblasts cell line - by Bioz Stars, 2026-03
    95/100 stars

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    1) Product Images from "Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts"

    Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts

    Journal: Frontiers in Molecular Biosciences

    doi: 10.3389/fmolb.2025.1720876

    Glutamate reprogram keloid fibroblasts transcripts. (A) Volcano plot show DEGs in HKF cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .
    Figure Legend Snippet: Glutamate reprogram keloid fibroblasts transcripts. (A) Volcano plot show DEGs in HKF cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .

    Techniques Used: Protein-Protein interactions

    Glutamate promotes inflammatory responses in keloid fibroblasts. (A–C) Volcano plot show DEGs that associated to cytokine activity pathway (A) , extracellular space pathway (B) , and inflammatory response pathway (C) in HKF cells upon 100 μM glutamate stimulate for 24 h. (D–F) Volcano plot and heatmap show DEGs that associated to TNF signaling pathway (D) , IL-17 signaling pathway (E) , and calcium signaling pathway (F) in HKF cells upon 100 μM glutamate stimulate for 24 h. (G) FPKM of inflammatory genes (CXCL8, CXCL3, CXCL2, IL11, IL6) of RNA-seq in keloid and normal tissues in HKF cells upon 100 μM glutamate stimulate for 24 h. (H) RT-PCR of inflammatory genes in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h. (I) ELISA of IL-6 andIL-11 in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.
    Figure Legend Snippet: Glutamate promotes inflammatory responses in keloid fibroblasts. (A–C) Volcano plot show DEGs that associated to cytokine activity pathway (A) , extracellular space pathway (B) , and inflammatory response pathway (C) in HKF cells upon 100 μM glutamate stimulate for 24 h. (D–F) Volcano plot and heatmap show DEGs that associated to TNF signaling pathway (D) , IL-17 signaling pathway (E) , and calcium signaling pathway (F) in HKF cells upon 100 μM glutamate stimulate for 24 h. (G) FPKM of inflammatory genes (CXCL8, CXCL3, CXCL2, IL11, IL6) of RNA-seq in keloid and normal tissues in HKF cells upon 100 μM glutamate stimulate for 24 h. (H) RT-PCR of inflammatory genes in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h. (I) ELISA of IL-6 andIL-11 in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Techniques Used: Activity Assay, RNA Sequencing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay



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    Image Search Results


    Glutamate reprogram keloid fibroblasts transcripts. (A) Volcano plot show DEGs in HKF cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .

    Journal: Frontiers in Molecular Biosciences

    Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts

    doi: 10.3389/fmolb.2025.1720876

    Figure Lengend Snippet: Glutamate reprogram keloid fibroblasts transcripts. (A) Volcano plot show DEGs in HKF cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .

    Article Snippet: The HKF (Human keloid fibroblasts) cell line obtained from American Type Culture Collection (CRL-1762, Manassas, VA, United States), and were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Carlsbad, CA, United States) containing 10% fetal bovine serum (FBS; Life Technologies) at 37 °C in a 5% humidified CO 2 incubator.

    Techniques: Protein-Protein interactions

    Glutamate promotes inflammatory responses in keloid fibroblasts. (A–C) Volcano plot show DEGs that associated to cytokine activity pathway (A) , extracellular space pathway (B) , and inflammatory response pathway (C) in HKF cells upon 100 μM glutamate stimulate for 24 h. (D–F) Volcano plot and heatmap show DEGs that associated to TNF signaling pathway (D) , IL-17 signaling pathway (E) , and calcium signaling pathway (F) in HKF cells upon 100 μM glutamate stimulate for 24 h. (G) FPKM of inflammatory genes (CXCL8, CXCL3, CXCL2, IL11, IL6) of RNA-seq in keloid and normal tissues in HKF cells upon 100 μM glutamate stimulate for 24 h. (H) RT-PCR of inflammatory genes in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h. (I) ELISA of IL-6 andIL-11 in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts

    doi: 10.3389/fmolb.2025.1720876

    Figure Lengend Snippet: Glutamate promotes inflammatory responses in keloid fibroblasts. (A–C) Volcano plot show DEGs that associated to cytokine activity pathway (A) , extracellular space pathway (B) , and inflammatory response pathway (C) in HKF cells upon 100 μM glutamate stimulate for 24 h. (D–F) Volcano plot and heatmap show DEGs that associated to TNF signaling pathway (D) , IL-17 signaling pathway (E) , and calcium signaling pathway (F) in HKF cells upon 100 μM glutamate stimulate for 24 h. (G) FPKM of inflammatory genes (CXCL8, CXCL3, CXCL2, IL11, IL6) of RNA-seq in keloid and normal tissues in HKF cells upon 100 μM glutamate stimulate for 24 h. (H) RT-PCR of inflammatory genes in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h. (I) ELISA of IL-6 andIL-11 in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: The HKF (Human keloid fibroblasts) cell line obtained from American Type Culture Collection (CRL-1762, Manassas, VA, United States), and were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Carlsbad, CA, United States) containing 10% fetal bovine serum (FBS; Life Technologies) at 37 °C in a 5% humidified CO 2 incubator.

    Techniques: Activity Assay, RNA Sequencing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay