clock gb111884 (Proteintech)

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Proteintech clock gb111884
Clock Gb111884, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fhl1/FHL1+Antibody/pmc12621143-89-24-26
Average 94 stars, based on 35 article reviews

clock gb111884 - by Bioz Stars, 2026-09

94/100 stars

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Bioprocessing:

Article Title: FHL1: A novel diagnostic marker for papillary thyroid carcinoma.
Article Snippet: .. Slides were immunostained with monoclonal antibodies against the BCL2 antigen (clone SP66, Maixin), CD117/C‐kit antigen (clone YR145, Maixin), and with monoclonal antibodies against the FHL1 (10991‐1‐AP, Proteintech Group, Inc.), PPARGC1A (Boster, China), and TLE1 antigen (Maixin). ..

Article Title: FHL1: A novel diagnostic marker for papillary thyroid carcinoma
Article Snippet: .. Slides were immunostained with monoclonal antibodies against the BCL2 antigen (clone SP66, Maixin), CD117/C‐kit antigen (clone YR145, Maixin), and with monoclonal antibodies against the FHL1 (10991‐1‐AP, Proteintech Group, Inc.), PPARGC1A (Boster, China), and TLE1 antigen (Maixin). ..

Membrane:

Article Title: Modulation of Titin and Contraction-Regulating Proteins in a Rat Model of Heart Failure with Preserved Ejection Fraction: Limb vs. Diaphragmatic Muscle
Article Snippet: Protein concentration was determined (BCA assay, Pierce, Bonn, Germany) and aliquots (10 μg) were separated by SDS-polyacrylamide gel electrophoresis. .. Proteins were transferred to a PVDF membrane and incubated overnight at 4 °C, using the following primary antibodies: SMYD2 (21290-1-AP, 1:2000), α-actinin (14221-1-AP, 1:2000), MYOT (10731-1-AP, 1:2000), FHL1 (10991-1-AP,1:1000), GDF-8 (19142-1-AP, 1:1000), tropomyosin (11038-1-AP, 1:2000), troponin C (13504-1-AP, 1:1000), troponin T (15513-1-AP, 1:1000) (all Proteintech, Planegg-Martinsried, Germany), troponin I (ab184554, 1:2000), telethonin (ab133646, 1:1000), MAFbx (ab168372, 1:1000) (all Abcam, Cambridge, UK), and MuRF-1 (Santa Cruz, Dallas, TX, USA, sc398608, 1:200). .. Membranes were subsequently incubated with a horseradish peroxidase-conjugated secondary antibody and specific bands were visualized by enzymatic chemiluminescence (Super Signal West Pico, Thermo Fisher Scientific Inc., Bonn, Germany).

Incubation:

Article Title: MyoMed205 Counteracts Titin Hyperphosphorylation and the Expression of Contraction‐Regulating Proteins in a Rat Model of HFpEF
Article Snippet: .. The membranes were incubated with the following primary antibodies overnight at 4°C: myosin light chain (MLC) 1 (15814‐1‐AP), SMYD2 (21290‐1‐AP, 1:2000), α‐actin (23660‐1‐AP), α‐actinin (14221‐1‐AP, 1:2000), MYOT (10731‐1‐AP, 1:2000), FHL1 (10991‐1‐AP, 1:1000), GDF‐8 (19142‐1‐AP, 1:1000), tropomyosin (11038‐1‐AP, 1:2000), troponin C (13504‐1‐AP, 1:1000), troponin T (19729‐1‐AP, 1:1000) (all Proteintech, Planegg‐Martinsried, Germany), troponin I (ab184554, 1:2000), telethonin (ab184554, 1:1000), MAFbx (ab168372, 1:1000), UbiK48 (ab140601, 1:1000) (all Abcam, Cambridge, UK), MuRF1 (Santa Cruz, Dallas, TX, USA, sc398608, 1:200), titin N2A (Myomedix, Neckargemünd, Germany, 1 μg/mL) and ph‐ S11878 (generously provided by Prof. Dr. W. Linke, University Münster, 1:1000). .. The next day, the membranes were incubated with a horseradish peroxidase‐conjugated secondary antibody, and the protein signal was visualized via enzymatic chemiluminescence (Super Signal West Pico, Thermo Fisher Scientific Inc., Bonn, Germany).

other:

Article Title: Inhibition of IL-11 signalling extends mammalian healthspan and lifespan
Article Snippet: Adiponectin (AdipoQ, 21613-1-AP, Proteintech), p-AMPK Thr172 (2535, clone 40H9, CST), AMPK (5832, clone D63G4, CST), CD31 (ab222783, clone EPR17260-263, abcam), CD68 (ab125212, abcam), cyclin D1 (55506, clone E3P5S, CST), p-ERK1/2 Thr202/Tyr204 (4370, clone D13.14.4E, CST), ERK1/2 (4695, clone 137F5, CST), GAPDH (2118, clone 14C10, CST), FHL1 (10991-1-AP, Proteintech), GFP (ab290 and ab6673, abcam), p-LKB1 Ser428 (3482, clone C67A3, CST), LKB1 (3047, clone D60C5, CST), p-mTOR Ser2448 (2971, CST), mTOR (2972,CST), p-NF-κB p65 Ser536 (3033, clone 93H1, CST), NF-κB p65 (8242, clone D14E12, CST), p16 (human, ab108349, clone EPR1473, abcam), p16 (mouse, ab232402, clone EPR20418, abcam), p21 (human, ab109520, clone EPR362, abcam), p21 (mouse, ab188224, clone EPR18021, abcam), p-p70S6K Thr389 (9234, clone 108D2, CST), p70S6K (2708, clone 49D7, CST), p-p90RSK Ser380 (11989, clone D3H11, CST), p90RSK (9355, clone 32D7, CST), PDGFRα (AF1062, R&D systems), p-S6 ribosomal protein Ser235/236 (4858, clone D57.2.2E, CST), S6 ribosomal protein (2217, clone 5G10, CST), PCNA (13110, clone D3H8P, CST), PGC1α (ab191838, abcam), SLC10A1 (MBS177905, MyBioSource), SM22α (ab14106, abcam), p-STAT3 Tyr705 (4113, clone M9C6, CST), STAT3 (4904, clone 79D7, CST), UCP1 (72298, clone E9Z2V, CST), anti-rabbit horseradish peroxidase (HRP) (7074, CST), anti-mouse HRP (7076, CST), anti-rabbit Alexa Fluor 488 (ab150077, abcam), anti-goat Alexa Fluor 488 (ab150129, abcam) and anti-rabbit Alexa Fluor 555 (ab150074, abcam).

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