fgfbp1  (Proteintech)

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: Validation of FGFBP1 overexpression and knockdown efficiency in cultured cells. ( A ) Quantitative analysis of FGFBP1 mRNA levels following genetic overexpression. ( B ) Assessment of FGFBP1 mRNA reduction efficiency after targeted knockdown. ( C , D ) Protein validation after overexpression and knockdown. Statistical significance is denoted as follows: * p < 0.05, and *** p < 0.001.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: Biomarker Discovery, Over Expression, Knockdown, Cell Culture

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: Proliferation of marker genes. ( A ) Effect of FGFBP1 overexpression on proliferation marker genes. ( B ) Effect of FGFBP1 knockdown on proliferation marker genes. Statistical significance is denoted as follows: ns ( p > 0.05), * p < 0.05, and *** p < 0.001.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: Marker, Over Expression, Knockdown

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: CCK-8 assay results following FGFBP1 overexpression and knockdown. ( A ) Effect of FGFBP1 overexpression on pre-adipocyte viability. ( B ) Effect of FGFBP1 knockdown on pre-adipocyte viability. Statistical significance is denoted as follows: * p < 0.05.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: CCK-8 Assay, Over Expression, Knockdown

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: Changes in cell number following FGFBP1 overexpression and knockdown as detected by EdU assay. ( A ) Cell proliferation following FGFBP1 overexpression. ( B ) Cell proliferation following FGFBP1 knockdown. Statistical significance is denoted as follows: ns ( p > 0.05), * p < 0.05.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: Over Expression, Knockdown, EdU Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: Flow cytometry analysis of cell cycle changes following FGFBP1 overexpression and knockdown. ( A ) Cell cycle profile of pcDNA3.1-transfected cells. ( B ) Cell cycle profile of pcDNA3.1- FGFBP1 -transfected cells. ( C ) Quantitative analysis of the cell cycle after FGFBP1 overexpression. ( D ) Cell cycle profile of si-NC-transfected cells. ( E ) Cell cycle profile of si- FGFBP1 -transfected cells. ( F ) Quantitative analysis of the cell cycle after FGFBP1 knockdown. Statistical significance is denoted as follows: ** p < 0.01 and *** p < 0.001.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: Flow Cytometry, Over Expression, Knockdown, Transfection

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: Changes in differentiation marker genes following FGFBP1 overexpression and knockdown. ( A ) Effect of FGFBP1 overexpression on differentiation marker genes. ( B ) Effect of FGFBP1 knockdown on differentiation marker genes. Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: Marker, Over Expression, Knockdown

Journal: Animals : an Open Access Journal from MDPI

Article Title: Decoding the Function of FGFBP1 in Sheep Adipocyte Proliferation and Differentiation

doi: 10.3390/ani15101456

Figure Lengend Snippet: Results of Oil Red O staining after FGFBP1 overexpression and knockdown. ( A ) Image of pcDNA3.1 transfection. ( B ) Image of pcDNA3.1- FGFBP1 transfection. ( C ) Quantitative analysis of adipogenesis via spectrophotometric measurement of Oil Red O content at 500 nm wavelength. ( D ) Image of si-NC transfection. ( E ) Image of si- FGFBP1 transfection. ( F ) Quantitative analysis of adipogenesis via spectrophotometric measurement of Oil Red O content at 500 nm wavelength. Statistical significance is denoted as follows: * p < 0.05 and ** p < 0.01.

Article Snippet: Next, the membranes were sequentially incubated overnight with the primary antibodies FGFBP1 Rabbit pAb (Catalog #BS-1768R, Bioss Antibodies, Beijing, China) at 4 °C and horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. β-actin was used as an internal control for normalization purposes.

Techniques: Staining, Over Expression, Knockdown, Transfection

Journal: FASEB BioAdvances

Article Title: COMMD10 Regulates Angiogenesis and Bone Formation via Rap1 Signaling Pathway

doi: 10.1096/fba.2024-00159

Figure Lengend Snippet: COMMD10 regulates the expression of angiogenesis‐related genes and proteins in endothelial cells; COMMD10 knockdown in endothelial cells increased tube formation. (A) Heat map showing expression levels of genes associated with angiogenesis. (B) Expression of proangiogenic genes ( Fgfbp1, Adra2b, Vegfa ) and anti‐angiogenic genes ( Cfh, Sema6a ) ( n = 3 per group). (C) Protein expression level of FGFBP1 was increased upon COMMD10 knockdown. (D) Representative 3D spheroid angiogenesis assay images. (E) Sprouts genesis of cell spheroids was quantified by measuring the number of sprouts per spheroid and the sprout length of all sprouts per spheroid ( n = 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The primary antibodies included COMMD10 (Abcam, UK, 1:1000), RAP1A + B (Abcam, UK, 1:5000), FGFBP1 (Abclonal, China, 1:1000), IGF1 (Abclonal, China, 1:1000), FGF18 (Abcam, China, 1:1000), Tubulin (Abclonal, China, 1:5000), and GAPDH (SAB, USA, 1:5000).

Techniques: Expressing, Knockdown, Angiogenesis Assay

Journal: FASEB BioAdvances

Article Title: COMMD10 Regulates Angiogenesis and Bone Formation via Rap1 Signaling Pathway

doi: 10.1096/fba.2024-00159

Figure Lengend Snippet: COMMD10 knockdown may promote angiogenesis via Rap1 signaling. (A) Expression of genes in the Rap1 signaling pathway. COMMD10 knockdown increased the expression of Fgf18 , Itgb2 , Fgfr1 , and Pard6b ( n = 3 per group). (B) Expression of protein in the Rap1 signaling pathway. COMMD10 knockdown increased the expression levels of fibroblast growth factor 18 (FGF18). (C) Commd10 and Rap1b mRNA expression levels. Commd10 was significantly knocked down in the SiCOMMD10 group and DK group. Rap1b gene was only significantly knocked down in the DK group ( n = 3 per group). (D) The angiogenesis‐related genes Fgfbp1 and Vegfa were expressed significantly higher in the SiCOMMD10 group, while no significant differences were found between the NC group and DK group. (E) The expression level of the secretory protein gene Igf1 was lower in the DK group than in the SiCommd10 group. The expression level of the secretory protein gene inhibiting osteogenesis, Ccl5 , was significantly upregulated after Rap1b knockdown ( n = 3 per group). “ns”= no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The primary antibodies included COMMD10 (Abcam, UK, 1:1000), RAP1A + B (Abcam, UK, 1:5000), FGFBP1 (Abclonal, China, 1:1000), IGF1 (Abclonal, China, 1:1000), FGF18 (Abcam, China, 1:1000), Tubulin (Abclonal, China, 1:5000), and GAPDH (SAB, USA, 1:5000).

Techniques: Knockdown, Expressing