fgf18  (Proteintech)

Journal: Bioactive Materials

Article Title: Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration

doi: 10.1016/j.bioactmat.2025.11.041

Figure Lengend Snippet: Spatiotemporal remodeling of FGF signaling networks in chondrocyte-like subpopulations. A) Heatmap of FGF signaling mediated communication patterns across chondrocyte-like subpopulations. B) Circle plot visualizing differential inter-subpopulation communication of FGF ligand-receptor signaling in chondrocyte-like cells at 4 week. C) Circle plot visualizing differential inter-subpopulation communication of FGF ligand-receptor signaling in chondrocyte-like cells at 8 week. D) Violin plots of expressions of FGF genes. E) Bubble plot of dysregulated ligand-receptor pairs. F) The differences in the overall information flow in the network. G) Representative western blots showing protein levels of FGF2 and FGF18 in Control and CE-SKP co-cultured cells.

Article Snippet: After blocking the membranes for an hour at room temperature using fast blocking solution, the membranes were incubated with primary antibodies specific for SOX9 (1:2000, 67439-1-Ig, Proteintech), COL2 (1:200, NBP1-91056, Novus Biologicals), COL10 (1:500, 26984-1-AP, Proteintech), COL1 (1:2000, 67288-1-Ig, Proteintech), RUNX2 (1:200, 20700-1-AP, Proteintech), FGF2 (1:500, 11234-1-AP, Proteintech), FGF18 (1:500, 60341-1-Ig, Proteintech) and GAPDH (1:50000, 60004-1-Ig, Proteintech) for an entire night at 4 °C.

Techniques: Western Blot, Control, Cell Culture

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

Article Snippet: After 24 h of stimulation, the supernatant was collected and the amount of Fibroblast Growth Factor 18 (FGF18) protein in the supernatant was measured using Human FGF18 ELISA set (Elabscience Biotechnology Inc.).

Techniques: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

Article Snippet: After 24 h of stimulation, the supernatant was collected and the amount of Fibroblast Growth Factor 18 (FGF18) protein in the supernatant was measured using Human FGF18 ELISA set (Elabscience Biotechnology Inc.).

Techniques: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Fibroblast growth factor 18 stimulates chondrocyte proliferation by modulating FOXN2 to mitigate post-traumatic osteoarthritis in a mouse model

doi: 10.3389/fbioe.2025.1615124

Figure Lengend Snippet: The expression pattern of FGF18 in PTOA and normal control cartilage. (a) Representative gross appearance of cartilage of PTOA patients and normal control patients. (b) The analysis of FGF18 expression in the cartilage of PTOA and normal control patients using immunohistochemical staining. (c) qRT-PCR of the relative expression of FGF18 in the cartilage PTOA and normal control patients. (d) Western blot analysis illustrating the expression of FGF18 in the cartilage of PTOA and normal control patients.

Article Snippet: After incubation with anti-FGF18 antibody (1:1000, Proteintech) or anti-GADPH (1:1000, Cell Signaling Technology, United States).

Techniques: Expressing, Control, Immunohistochemical staining, Staining, Quantitative RT-PCR, Western Blot

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Fibroblast growth factor 18 stimulates chondrocyte proliferation by modulating FOXN2 to mitigate post-traumatic osteoarthritis in a mouse model

doi: 10.3389/fbioe.2025.1615124

Figure Lengend Snippet: FGF18 regulates the proliferation of chondrocytes. (a) Analysis of transfection efficiency of pcDNA3.1-FGF18 in human chondrocytes using green fluorescent protein (GFP). (b) Immunoblot analysis illustrating the expression levels of FGF18 in human chondrocytes transfected with pcDNA3.1-FGF18, FGF18 siRNA, or their corresponding controls. n = 6 independent biological replicates per group. (c) Analysis of human chondrocyte apoptosis transfected with indicated plasmids assayed by flow cytometry. n = 6 independent biological replicates per group. (d) Analysis of human chondrocyte proliferation transfected with indicated plasmids assayed by EdU assay. n = 6 independent biological replicates per group.

Article Snippet: After incubation with anti-FGF18 antibody (1:1000, Proteintech) or anti-GADPH (1:1000, Cell Signaling Technology, United States).

Techniques: Transfection, Western Blot, Expressing, Flow Cytometry, EdU Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Fibroblast growth factor 18 stimulates chondrocyte proliferation by modulating FOXN2 to mitigate post-traumatic osteoarthritis in a mouse model

doi: 10.3389/fbioe.2025.1615124

Figure Lengend Snippet: FGF18 regulates the expression level of FOXN2 and PTOA-associated factors. (a) Heat map demonstrating differentially expressed genes in FGF18-knockout chondrocytes compared to normal controls. Statistical significance was determined using DESeq2 with adjusted P < 0.05 and |log2 Fold Change|>1. (b) Western blot analysis illustrating the expression levels of FGF18 in human chondrocytes transfected with indicated plasmids. n = 6 independent biological replicates per group. FOXN2 was the target gene of FGF18. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. corresponding controls. (c,d) Analysis of BMP-2 and CTX-II expression in human chondrocytes transfected with indicated plasmids using immunofluorescence assay.

Article Snippet: After incubation with anti-FGF18 antibody (1:1000, Proteintech) or anti-GADPH (1:1000, Cell Signaling Technology, United States).

Techniques: Expressing, Knock-Out, Western Blot, Transfection, Immunofluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Fibroblast growth factor 18 stimulates chondrocyte proliferation by modulating FOXN2 to mitigate post-traumatic osteoarthritis in a mouse model

doi: 10.3389/fbioe.2025.1615124

Figure Lengend Snippet: FGF18 regulates cartilage development and attenuates PTOA development. (a) Representative images of cartilage in DMM mice after treatment with FGF18 stained using Safranin O (n = 6 per group). Scale bar = 100 μm. (b) Analysis of CTX-II and Aggrecan expression in the cartilage of DMM mice after treatment with FGF18 using immunofluorescence assay. Primary antibodies: anti-CTX-II (1:500; ab3092; Abcam) and anti-Aggrecan (1:500; ab36861; Abcam), followed by FITC-conjugated secondary antibody (1:1000; Thermo Fisher Scientific). Nuclei were counterstained with DAPI. Scale bar = 50 μm (n = 6 per group). (c) Analysis of MMP13 and ADAMTS-5 in the cartilage of DMM mice after treatment with FGF18 using immunofluorescence assay. Primary antibodies: anti-MMP13 (1:500; ab39012; Abcam) and anti-ADAMTS-5 (1:500; ab41037; Abcam), followed by FITC-conjugated secondary antibody (1:1000; Thermo Fisher Scientific). Nuclei were counterstained with DAPI. Scale bar = 50 μm (n = 6 per group).

Article Snippet: After incubation with anti-FGF18 antibody (1:1000, Proteintech) or anti-GADPH (1:1000, Cell Signaling Technology, United States).

Techniques: Staining, Expressing, Immunofluorescence

Journal: Nature Communications

Article Title: Transgenerational inheritance of diminished ovarian reserve triggered by prenatal propylparaben exposure in mice

doi: 10.1038/s41467-025-63440-z

Figure Lengend Snippet: a STRING database revealed strong interactions between RhoBTB1 and FGF18, RhoBTB1 and CTSK, respectively. b , c The protein levels of FGF18 and MAPK signaling pathway in KGN cells detected by western blotting following PrP exposure. Data from ( c ) are representative of three independent experiments (mean ± SEM; one-way ANOVA). d , e The protein levels of FGF18 and MAPK signaling pathway in KGN cells detected by western blotting following RhoBTB1 over-expression. Data from ( e ) are representative of three independent experiments (mean ± SEM; unpaired two-tailed t -test). f , g The protein levels of FGF18, RhoBTB1, and MAPK signaling pathway in GCs detected by western blotting following PrP exposure and siRNA-F02 interference of Fgf18 . Data from ( g ) are representative of three independent experiments (mean ± SEM; one-way ANOVA). h , i The protein levels of RhoBTB1, FGF18, and MAPK signaling pathway in GCs detected by western blotting following PrP exposure and siRNA-R03 interference of Rhobtb1 . Data from ( i ) are representative of three independent experiments (mean ± SEM; one-way ANOVA). j , k The protein levels of FGF18 in GCs following PrP exposure and MLN4924 treatment. Data from ( k ) are representative of three independent experiments (mean ± SEM; one-way ANOVA). l , m The protein levels of FGF18 in GCs following RhoBTB1 over-expression and MLN4924 treatment. Data from ( m ) are representative of three independent experiments (mean ± SEM; unpaired two-tailed t -test). n , o The protein levels of FGF18 in GCs following PrP exposure and MG132 treatment. Data from ( o ) are representative of three independent experiments (mean ± SEM; one-way ANOVA). p , q The protein levels of FGF18 in GCs following RhoBTB1 over-expression and MG132 treatment. Data from ( q ) are representative of three independent experiments (mean ± SEM; unpaired two-tailed t -test). The samples derived from the same experiment and that blots were processed in parallel. Source data are provided as a Source Data file.

Article Snippet: The Antibodies were purchased from different companies: Cell Signaling Technology for ERK (Extracellular Signal-Regulated Kinase; 4695T), p-ERK (Phosphorylated ERK; 4370T), p38 (p38 Mitogen-Activated Protein Kinase; 8690T), p-p38 (Phosphorylated p38; 4511T), JNK (c-Jun N-terminal Kinase; 9252T), and p-JNK (Phosphorylated JNK; 4668T); Abclonal for β-ACTIN (AC038), active-Caspase3 (A19654), BCL-2 (A19693), BAX (A12009), SOD2 (A19576), BMP15 (A7321), CAT (A11777), HSD17B7 (A17157), and CTSK (A1782); Proteintech for FGF18 (60341-1-lg); Immunoway for RhoBTB1 (YT4084); Servicebio for HRP conjugated goat anti-rabbit IgG (GB23303), and Alexa Fluor® 488-conjugated goat anti-rabbit IgG (GB25303).

Techniques: Western Blot, Over Expression, Two Tailed Test, Derivative Assay