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medchemexpress cat hy 136485  (MedChemExpress)


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    MedChemExpress medchemexpress cat hy 136485
    Medchemexpress Cat Hy 136485, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medchemexpress cat hy 136485/product/MedChemExpress
    Average 93 stars, based on 3 article reviews
    medchemexpress cat hy 136485 - by Bioz Stars, 2026-02
    93/100 stars

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    hFen1 immunodepletion increased SP-BER product. ( A ) The BER patch size for a U:A and rAP:A plasmid substrate generated by asynchronous HEK 293 FT WCE or WCE supplemented with Polβ (50 nM) and aphidicolin (100 μg/ml). Aph, aphidicolin. **** indicates P < .0001. n = 3 independent experiments and the data are means ± SD. Async, asynchronous. ( B ) The hFen1 abundance in asynchronous HEK 293 FT WCE and anti-hFen1 antibody immunodepleted WCE were determined using western blot. ID: Immunodepletion. ( C ) The BER patch size for a U:A plasmid substrate generated by asynchronous HEK 293 FT WCE, hFen1 immunodepleted WCE, hFen1 (150 nM) supplemented hFen1 immunodepleted WCE, and xFen1 (150 nM) supplemented hFen1 immunodepleted WCE. **** indicates P < .0001. ns, not significant. n = 3 independent experiments and the data are means ± SD.

    Journal: Nucleic Acids Research

    Article Title: Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathways

    doi: 10.1093/nar/gkaf1326

    Figure Lengend Snippet: hFen1 immunodepletion increased SP-BER product. ( A ) The BER patch size for a U:A and rAP:A plasmid substrate generated by asynchronous HEK 293 FT WCE or WCE supplemented with Polβ (50 nM) and aphidicolin (100 μg/ml). Aph, aphidicolin. **** indicates P < .0001. n = 3 independent experiments and the data are means ± SD. Async, asynchronous. ( B ) The hFen1 abundance in asynchronous HEK 293 FT WCE and anti-hFen1 antibody immunodepleted WCE were determined using western blot. ID: Immunodepletion. ( C ) The BER patch size for a U:A plasmid substrate generated by asynchronous HEK 293 FT WCE, hFen1 immunodepleted WCE, hFen1 (150 nM) supplemented hFen1 immunodepleted WCE, and xFen1 (150 nM) supplemented hFen1 immunodepleted WCE. **** indicates P < .0001. ns, not significant. n = 3 independent experiments and the data are means ± SD.

    Article Snippet: To achieve immunodepletion of hFen1 in 1 mg WCE (60 μl under our experimental conditions), 50 μl of Protein A Dynabeads (Invitrogen, 10001D) and 25 μl of hFen1 antibody (Proteintech, 14768-1-AP) were utilized.

    Techniques: Immunodepletion, Plasmid Preparation, Generated, Western Blot

    hFen1 immunodepletion increased SP-BER product. ( A ) The BER patch size for a U:A and rAP:A plasmid substrate generated by asynchronous HEK 293 FT WCE or WCE supplemented with Polβ (50 nM) and aphidicolin (100 μg/ml). Aph, aphidicolin. **** indicates P < .0001. n = 3 independent experiments and the data are means ± SD. Async, asynchronous. ( B ) The hFen1 abundance in asynchronous HEK 293 FT WCE and anti-hFen1 antibody immunodepleted WCE were determined using western blot. ID: Immunodepletion. ( C ) The BER patch size for a U:A plasmid substrate generated by asynchronous HEK 293 FT WCE, hFen1 immunodepleted WCE, hFen1 (150 nM) supplemented hFen1 immunodepleted WCE, and xFen1 (150 nM) supplemented hFen1 immunodepleted WCE. **** indicates P < .0001. ns, not significant. n = 3 independent experiments and the data are means ± SD.

    Journal: Nucleic Acids Research

    Article Title: Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathways

    doi: 10.1093/nar/gkaf1326

    Figure Lengend Snippet: hFen1 immunodepletion increased SP-BER product. ( A ) The BER patch size for a U:A and rAP:A plasmid substrate generated by asynchronous HEK 293 FT WCE or WCE supplemented with Polβ (50 nM) and aphidicolin (100 μg/ml). Aph, aphidicolin. **** indicates P < .0001. n = 3 independent experiments and the data are means ± SD. Async, asynchronous. ( B ) The hFen1 abundance in asynchronous HEK 293 FT WCE and anti-hFen1 antibody immunodepleted WCE were determined using western blot. ID: Immunodepletion. ( C ) The BER patch size for a U:A plasmid substrate generated by asynchronous HEK 293 FT WCE, hFen1 immunodepleted WCE, hFen1 (150 nM) supplemented hFen1 immunodepleted WCE, and xFen1 (150 nM) supplemented hFen1 immunodepleted WCE. **** indicates P < .0001. ns, not significant. n = 3 independent experiments and the data are means ± SD.

    Article Snippet: Rabbit polyclonal anti-hFen1 antibody (1:500, Proteintech, 14768-1-AP), rabbit polyclonal anti-xFen1 antibody (1:1000, see the “Acknowledgments” section), rabbit polyclonal anti-hPolβ antibody (1:250, Novus, NBP2-38600), rabbit polyclonal anti-GAPDH antibody (1:1000, Rockland, 600-401-A33), and IRDye 800CW labeled goat anti-rabbit IgG (1:10 000, LI-COR, 926-32211) were diluted in 1% non-fat milk in TBS-T.

    Techniques: Immunodepletion, Plasmid Preparation, Generated, Western Blot