Journal: International journal of legal medicine
Article Title: Hypothermia drives fatty acid transporter 1 upregulation and lipid accumulation in renal tubules: evidence from forensic cases.
doi: 10.1007/s00414-025-03550-x
Figure Lengend Snippet: Fig. 3 Effect of low temperature on gene expression and lipid accumu lation in cultured human proximal tubular cells. (a–c) Comparison of gene expression in HK2 cells cultured at 37 °C and 28 °C, as analyzed by real-time RT-PCR for (a) SLC27A1 mRNA, (b) PPARA mRNA, and CD36 mRNA (n = 6 per condition). (c) Western blot analysis of FATP1 and β-actin proteins in HK2 cells cultured at 37 °C and 28 °C, with quantitative data derived from western blotting (n = 6 per condition). (d) Analysis of lipid accumulation in HK2 cells cultured at 37 °C and 28 °C with 0.1 mM palmitic acid (PA) and/ or a FATP1 inhibitor. Lipids are visualized using BODIPY (green), with nuclei counterstained with DAPI (blue). Scale bars: 10 μm. (e) Quantitative analysis of fluores cence intensity representing lipid accumulation in HK2 cells cultured at 37 °C and 28 °C (n = 12 per con dition). Data were analyzed using one-way ANOVA followed by the Mann–Whitney U test. All data are presented as mean ± SEM. *P < 0.05, ****P < 0.0001
Article Snippet: Sections were then incubated with a rabbit polyclonal FATP1 primary antibody (Novus Biologicals, Littleton, CO, USA), followed by detection with a horseradish peroxidase (HRP)-conjugated secondary rabbit IgG antibody (Vector Labs, Burlingame, CA, USA).
Techniques: Gene Expression, Cell Culture, Comparison, Quantitative RT-PCR, Western Blot, Derivative Assay, MANN-WHITNEY
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