anti engrailed 1  (Bioss)

Journal: Scientific Reports

Article Title: Region-specific roles of stress-activated protein kinase MKK7 in the developing and maturing murine brain

doi: 10.1038/s41598-025-05388-0

Figure Lengend Snippet: Construction of brain region-specific Mkk7 cKO mice and phenotypes of Mkk7 flox/flox En1-Cre mice. ( A ) Scheme of mating of Cre recombinase-expressing mice with ROSA-tdTomato mice. When recombination occurs due to Cre recombinase expressed from the region-specific promoter, tdTomato is expressed. ( B ) Macroscopic images of brains (left) and spinal cords (right) from mice of the indicated genotypes. ( C ) Cresyl violet staining of sagittal sections of control and Mkk7 flox/flox En1-Cre brains at E18.5. ( D ) Tyrosine hydroxylase (TH) staining of sagittal sections of control and Mkk7 flox/flox En1-Cre brains at E18.5. ( E ) Quantification of the: (i) ratio of the brain lateral ventricle area relative to the whole brain area; (ii) ratio of the cerebellum area relative to the whole brain area; (iii) midbrain thickness; (iv) ratio of the subpallium area relative to the whole brain area for the brains in (C); (v) TH-positive area of the mutant brain relative to control brain as shown in (D). n.s., not significant.

Article Snippet: Mkk7 flox/flox mice were crossed to Hoxb8-Cre (kindly provided by Dr. Zeilhofer, University of Zurich) , En1-Cre (The Jackson Laboratory) , or Emx1-Cre (Riken) transgenic mice expressing Cre recombinase under the control of the Hoxb8 , En1 , or Emx1 promotor, respectively.

Techniques: Expressing, Staining, Control, Mutagenesis

Journal: bioRxiv

Article Title: Development of a protocol utilizing single-cell analysis for the differentiation of human iPSCs into SOX6+ midbrain dopaminergic neurons

doi: 10.1101/2025.06.18.660098

Figure Lengend Snippet: (A) Schematic depiction of the protocol for differentiating mDA neurons, illustrating the application of pharmacological compounds and growth factors. Representative immunocytochemical images and quantification from both original protocol and new protocol showing (B, E) efficacies of midbrain floorplate patterning at DIV11, showing co-localizations of LMX1+/EN1+ and SOX6+/OTX2+; and EN1+/SOX6+/TH+ neurons at DIV 16 (C, F) and 28 (D, G). White scale bars = 50, 100 µm as indicated (n = 3 independent experiments, error bars are SEM, ns = not significant; * = p<0.05; ** = p<0.01; *** = p<0.001; **** = p<0.0001). (H) Calcium imaging recordings of spontaneous and KCl-induced activities on DIV 28 and 42, presenting the total number of calcium events per neuron recorded, and the percentages of cells responding to KCl-induced depolarization. Metrics of activities, including amplitude, rise time, and fall time of calcium signals, are depicted in the subsequent bar plots.

Article Snippet: The primary antibodies were used as follows: CORIN (rat, 1:1,000, R&D Systems, MAB2209), DCX (goat, 1:500, SantaCruz, sc-8066), EN1 (mouse 1:50, DSHB, 4G11), FOXA2 (goat, 1:500, R&D Systems, AF2400), GIRK2 (rabbit, 1:400, Alomone, APC006), LMX1A (rabbit, 1:4,000, Millipore, AB10533), LMO3 (goat, 1:200, SantaCruz, sc-82647), MAP2 (mouse 1:1,000, Sigma, M4403), NURR1 (rabbit, 1:500, SantaCruz, sc-990), OTX2 (goat, 1:1,000, R&D Systems, AF1979), TH (rabbit, 1:1,000, Millipore, AB152), TH (mouse, 1:500, ImmunoStar, 22941), and TH (sheep, 1:500, Novus, NB300).

Techniques: Imaging