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b subtilis 3610  (ATCC)


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    Structured Review

    ATCC b subtilis 3610
    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
    B Subtilis 3610, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b subtilis 3610/product/ATCC
    Average 95 stars, based on 326 article reviews
    b subtilis 3610 - by Bioz Stars, 2026-03
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    1) Product Images from "A 3D-printed capillary tube holder for high-throughput chemotaxis assays"

    Article Title: A 3D-printed capillary tube holder for high-throughput chemotaxis assays

    Journal: Journal of Bacteriology

    doi: 10.1128/jb.00384-25

    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus subtilis 3610 and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
    Figure Legend Snippet: Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus subtilis 3610 and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.

    Techniques Used: Construct, Concentration Assay



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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus <t>subtilis</t> <t>3610</t> and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
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    Image Search Results


    Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus subtilis 3610 and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.

    Journal: Journal of Bacteriology

    Article Title: A 3D-printed capillary tube holder for high-throughput chemotaxis assays

    doi: 10.1128/jb.00384-25

    Figure Lengend Snippet: Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus subtilis 3610 and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.

    Article Snippet: E. coli K12 (ATCC 25404) and B. subtilis 3610 (ATCC 6051) were inoculated into LB media and incubated at 37°C with shaking at 250 rpm for 16–18 hours.

    Techniques: Construct, Concentration Assay