egm-mv2 (Lonza)
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egm-mv2
Egm Mv2, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/egm-mv2/egm+mv2/pm40080391-54-10-12
Average 90 stars, based on 1 article reviews
Egm Mv2, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/egm-mv2/egm+mv2/pm40080391-54-10-12
Average 90 stars, based on 1 article reviews
egm-mv2 - by Bioz Stars,
2026-10
90/100 stars
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Purification:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Recombinant:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Cell Culture:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Negative Control:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Clinical Proteomics:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Imaging:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Incubation:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in Activity Assay:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in In Vitro:Article Title: Lasmiditan induces mitochondrial biogenesis in primary mouse renal peritubular endothelial cells and augments wound healing and tubular network formation. Article Snippet: Kidney disease (KD) is a progressive and life-threatening illness that has manifested into a global health crisis, impacting >10% of the general population.. Hallmarks of KD include tubular interstitial fibrosis, renal tubular cell atrophy/necrosis, glomerulosclerosis, persistent inflammation, microvascular endothelial cell (MV-EC) dysfunction/rarefaction, and mitochondrial dysfunction.. Following acute kidney injury (AKI), and/or during KD onset/progression, MV-ECs of the renal peritubular endothelial capillaries (RPECs) are highly susceptible to injury, dysfunction, and rarefaction. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in Article Title: Identification of a Unique Resorcylic Acid Lactone Derivative That Targets Both Lymphangiogenesis and Angiogenesis. Article Snippet: We synthesized 11 novel L-783277 derivatives, in which a structure rigidifying phenyl ring is incorporated into the 14-membered chiral resorcylic acid lactone system.. The SAR study with these substances demonstrated that 17 possesses excellent kinase selectivity against a panel of 335 kinases in contrast to L-783277 and inhibits VEGFR3, VEGFR2 and FLT3 with single-digit nanomolar IC50 values.. Also, we found that 21, a stereoisomer of 17, has excellent potency (IC50 = 9 nM) against VEGFR3 and selectivity over VEGFR2 and FLT3. Article Title: An endothelial activin A-bone morphogenetic protein receptor type 2 link is overdriven in pulmonary hypertension Article Snippet: PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: The following day, cells were gently washed twice with pre-warmed ( Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dish-high Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37°C with 5% CO 2 in Article Title: Loss of JCAD/KIAA1462 Protects the Lung from Acute and Chronic Consequences of Chronic Obstructive Pulmonary Disease Article Snippet: HPMECs were cultured in Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells. Article Snippet: Purified MRPECs were first seeded onto human plasma fibronectin (10 μg/ml/dish, PromoCell) precoated 35-mm μ-Dishhigh Ibidi imaging dishes or 35-mm Corning Primaria cell culture dishes and incubated overnight at 37◦C with 5% CO2 in |
