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luciferase assay kit promega e1500  (Promega)

 
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    Promega luciferase assay kit promega e1500
    Luciferase Assay Kit Promega E1500, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase assay kit promega e1500/product/Promega
    Average 90 stars, based on 1 article reviews
    luciferase assay kit promega e1500 - by Bioz Stars, 2026-03
    90/100 stars

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    luciferase detection system e1500  (Promega)
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    E2F1 is an Upstream Transcription Factor of EIF4EBP1. (A) Intersection analysis of EIF4EBP1 transcription factors (TFs) with data from GSE149084 and GSE25518 . (B) WB analysis of E2F1 expression, data analyzed using t-test. (C) WB analysis of EIF4EBP1 expression, data analyzed using t-test. (D) <t>Dual-luciferase</t> reporter assay to evaluate the effect of E2F1 on EIF4EBP1 promoter activity, data analyzed using Two-way ANOVA, and the post hoc test was conducted using Tukey’s. (E) Chromatin immunoprecipitation (ChIP) assay to determine the enrichment of the EIF4EBP1 promoter in E2F1, data analyzed using t-test. ns P >0.05; *** P < 0.001; **** P < 0.0001.
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    E2F1 is an Upstream Transcription Factor of EIF4EBP1. (A) Intersection analysis of EIF4EBP1 transcription factors (TFs) with data from GSE149084 and GSE25518 . (B) WB analysis of E2F1 expression, data analyzed using t-test. (C) WB analysis of EIF4EBP1 expression, data analyzed using t-test. (D) Dual-luciferase reporter assay to evaluate the effect of E2F1 on EIF4EBP1 promoter activity, data analyzed using Two-way ANOVA, and the post hoc test was conducted using Tukey’s. (E) Chromatin immunoprecipitation (ChIP) assay to determine the enrichment of the EIF4EBP1 promoter in E2F1, data analyzed using t-test. ns P >0.05; *** P < 0.001; **** P < 0.0001.

    Journal: Frontiers in Genetics

    Article Title: The role of E2F1 in promoting EIF4EBP1 transcription in cryptorchid mice: association with autophagy in germ cells

    doi: 10.3389/fgene.2025.1536672

    Figure Lengend Snippet: E2F1 is an Upstream Transcription Factor of EIF4EBP1. (A) Intersection analysis of EIF4EBP1 transcription factors (TFs) with data from GSE149084 and GSE25518 . (B) WB analysis of E2F1 expression, data analyzed using t-test. (C) WB analysis of EIF4EBP1 expression, data analyzed using t-test. (D) Dual-luciferase reporter assay to evaluate the effect of E2F1 on EIF4EBP1 promoter activity, data analyzed using Two-way ANOVA, and the post hoc test was conducted using Tukey’s. (E) Chromatin immunoprecipitation (ChIP) assay to determine the enrichment of the EIF4EBP1 promoter in E2F1, data analyzed using t-test. ns P >0.05; *** P < 0.001; **** P < 0.0001.

    Article Snippet: Instruments: ELISA Reader (1410101, Thermo Fisher Scientific, MA, United States); Fluorescence Microscope (DM2500, Leica, Wetzlar, Germany); BeyoECL Star (A38554, Thermo Fisher, Massachusetts, United States); Luciferase Detection System (E1500, Promega, Madison, Wisconsin, United States); ImageJ Software (V1.8.0.112, NIH, Madison, WI, United States); FlowJo (v10.8.2, BD, Ashland, Oregon, United States); GraphPad Prism 9.0 (Dotmatics, Boston, MA, United States).

    Techniques: Expressing, Luciferase, Reporter Assay, Activity Assay, Chromatin Immunoprecipitation