Journal: bioRxiv
Article Title: Microtubule remodeling by the innate immune factor Trim69 compromises dynein-dependent migration of HIV virion cores towards the nucleus
doi: 10.1101/2025.02.06.636796
Figure Lengend Snippet: A) TZM-BL cells expressing or not Trim69 were challenged with GFP-coding and JR-FL Env-bearing single round of infection-competent HIV-1 virus following siRNA transfection of the indicated proteins (efficacy of silencing presented in Extended Data 9B). The extent of infection was measured by flow cytometry two to three days later. B) Schematic organization of the dynein and dynactin complexes along with the BICD2 adaptor. Red arrows indicate the components analyzed by confocal microscopy using either antibodies directed against endogenous proteins ( C) BICD2 and Extended Data 10 for DYNLL1), or using ectopic transfection of GFP- or mCherry-fusion components ( D ). The complete panel analysis is provided in the Extended Data 10). E) TZM-BL cells expressing or not DYNC1LI2 along with the SPRY-7A Trim69 mutant were obtained by transient transfection. Cells were then analyzed by confocal microscopy. The graph present averages and SEM while panels present typical images obtained (n= 3). * and ns, p<0.05 and non-significant following one-way Anova, Tukey’s multiple comparison tests between the indicated conditions.
Article Snippet: Plasmids expressing the following dynein and dynactin complex components were also obtained through Addgene: DYNC1LI1 and DYNC1LI2 respectively pLVXPuro-mGFP-LIC1/-LIC2 (cat #66600 and #66601).
Techniques: Expressing, Infection, Virus, Transfection, Flow Cytometry, Confocal Microscopy, Mutagenesis, Comparison
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