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Nacalai dulbecco
Dulbecco, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dulbecco/medium/pmc13199936-217-4-9
Average 86 stars, based on 1 article reviews
dulbecco - by Bioz Stars, 2026-10
86/100 stars

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Cell Culture:

Article Title: Topology-dependent FRET efficiency in living cells via N-C swapping of fluorescent protein fusions
Article Snippet: COS-1 cells were originally obtained from ATCC (Manassas, VA, USA) and kindly provided by the laboratory at Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine (Kyoto, Japan). .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 5% fetal bovine serum (FBS; Biosera, Cholet, France) and 1× penicillin-streptomycin (Nacalai) under a 5% CO 2 /95% air atmosphere and 100% humidity at 37°C. ..

Article Title: Enhancing Immunotherapeutic Response in Colorectal Cancer with a Neuropilin-1-Targeting Tumor-Penetrating Peptide
Article Snippet: Cell line authentication was not performed in this 129 study, and all cells were routinely tested for mycoplasma contamination using the 130 LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) and confirmed to be negative. .. 131 MC38 cells were cultured in Dulbecco’s modified Eagle medium (Nacalai Tesque, 132 08459-65), and CT26 cells were cultured in RPMI 1640 medium (Nacalai Tesque, 30264-133 85), both supplemented with 10% fetal bovine serum (MP Biomedicals, 2917354H) and 134 1% penicillin/streptomycin (FUJIFILM Wako, 168-23191) at 37°C in a humidified 135 atmosphere containing 5% CO2. .. NRP1 knockout MC38 cells (MC38-NRP1-KO) were 136 generated using the CRISPR-Cas9 system with a px458 plasmid (RRID: 137 Addgene_48138) encoding a guide RNA targeting the NRP1 gene.

Article Title: Quantitative dissection of radioresistance in cancer cells: Oxygen depletion-induced biological versus chemical mechanisms and baseline mechanisms governed by HIF-1β-NRF2/TXNRD1
Article Snippet: .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Nacalai Tesque) supplemented with 10% heat-inactivated fetal bovine serum (FBS; EQUITECH-BIO) and 1% (v/v) penicillin-streptomycin (Nacalai Tesque) at 37°C in a humidified incubator with 5% CO 2 and 95% air. .. Cells were cultured at < 0.1% O 2 , 5% CO 2 for chronically anoxic conditions, and at 1% O 2 , 5% CO 2 for chronically hypoxic conditions, respectively, for the indicated time in a RUSKINN INVIVO2 400 Hypoxic Workstation (Ruskinn Technology Limited).

Article Title: Genome instability is increased during DNA replication in the presence of the CPD UV photoproduct in transcriptionally active genes
Article Snippet: .. All MEFs were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 10% (v/v) fetal calf serum (FCS; Gibco, Waltham, MA, USA). ..

Modification:

Article Title: Topology-dependent FRET efficiency in living cells via N-C swapping of fluorescent protein fusions
Article Snippet: COS-1 cells were originally obtained from ATCC (Manassas, VA, USA) and kindly provided by the laboratory at Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine (Kyoto, Japan). .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 5% fetal bovine serum (FBS; Biosera, Cholet, France) and 1× penicillin-streptomycin (Nacalai) under a 5% CO 2 /95% air atmosphere and 100% humidity at 37°C. ..

Article Title: Selective elimination of amyloid-β-induced senescent neuroblastoma cells by Moringa oleifera leaf extract.
Article Snippet: .. Briefly, Dulbecco’s Modified Eagle Medium (DMEM, Nacalai Tesque) supplemented with 12% fetal bovine serum (FBS) and 1% penicillin-streptomycin were used to culture the cells at 37°C, in a humidified incubator with 5% CO2. ..

Article Title: A natural coumarin, benjaminin, suppresses proliferation and migration and induces apoptosis in MCF-7 breast cancer cells.
Article Snippet: The human breast adenocarcinoma cell line, Michigan Cancer Foundation-7 (MCF-7), and the immortalized normal human mammary epithelial cell line, Michigan Cancer Foundation-10A (MCF-10A), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Dulbecco’s Modified Eagle Medium/Ham’s F-12 Nutrient Mixture (DMEM/F-12), 0.25% trypsin/EDTA, and hydrocortisone were obtained from Nacalai Tesque (Japan). .. Dulbecco’s Modified Eagle Medium (DMEM) and the Annexin V-FITC Apoptosis Detection Kit were obtained from Elabscience (USA).

Article Title: Enhancing Immunotherapeutic Response in Colorectal Cancer with a Neuropilin-1-Targeting Tumor-Penetrating Peptide
Article Snippet: Cell line authentication was not performed in this 129 study, and all cells were routinely tested for mycoplasma contamination using the 130 LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) and confirmed to be negative. .. 131 MC38 cells were cultured in Dulbecco’s modified Eagle medium (Nacalai Tesque, 132 08459-65), and CT26 cells were cultured in RPMI 1640 medium (Nacalai Tesque, 30264-133 85), both supplemented with 10% fetal bovine serum (MP Biomedicals, 2917354H) and 134 1% penicillin/streptomycin (FUJIFILM Wako, 168-23191) at 37°C in a humidified 135 atmosphere containing 5% CO2. .. NRP1 knockout MC38 cells (MC38-NRP1-KO) were 136 generated using the CRISPR-Cas9 system with a px458 plasmid (RRID: 137 Addgene_48138) encoding a guide RNA targeting the NRP1 gene.

Article Title: Trace-Level Detection of a Niacin Metabolite in Human Blood under Phosphonate Pillar[6]arene-Induced Columnar-Like Aggregation Conditions.
Article Snippet: A rapid method for quantifying the niacin metabolite 1-methylnicotinamide (1-MNA) in human blood is required to address the limitations of existing low-throughput techniques such as mass spectrometry.. In previous studies, we demonstrated that supramolecular pillar[6]arenes (P6As) bearing either carboxylate groups (P6AC) or sulfonate groups (P6AS) functioned as “turn-off” fluorescent sensors for 1-MNA detection in urine by photoinduced electron transfer (PET).. However, because the concentration of 1-MNA in blood is very low, PET-based detection in aqueous solutions was insufficient for reliable quantification on its own.

Article Title: Quantitative dissection of radioresistance in cancer cells: Oxygen depletion-induced biological versus chemical mechanisms and baseline mechanisms governed by HIF-1β-NRF2/TXNRD1
Article Snippet: .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Nacalai Tesque) supplemented with 10% heat-inactivated fetal bovine serum (FBS; EQUITECH-BIO) and 1% (v/v) penicillin-streptomycin (Nacalai Tesque) at 37°C in a humidified incubator with 5% CO 2 and 95% air. .. Cells were cultured at < 0.1% O 2 , 5% CO 2 for chronically anoxic conditions, and at 1% O 2 , 5% CO 2 for chronically hypoxic conditions, respectively, for the indicated time in a RUSKINN INVIVO2 400 Hypoxic Workstation (Ruskinn Technology Limited).

Article Title: Genome instability is increased during DNA replication in the presence of the CPD UV photoproduct in transcriptionally active genes
Article Snippet: .. All MEFs were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 10% (v/v) fetal calf serum (FCS; Gibco, Waltham, MA, USA). ..

Saline:

Article Title: Development of Novel Lysosome-Targeting Chimera by Non-covalent-Type Fc-IGF2 Complex (Fc-LYTAC) for Target Membrane Protein Degradation.
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM) (high glucose) (Catalog #048-29765), WIDE-VIEW pre-stained protein size marker (Catalog #234-02464), and dithiothreitol (DTT; Catalog #042-29222) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). .. Dulbecco’s phosphate-buffered saline (PBS; Catalog #14249-24) was purchased from Nacalai Tesque Inc. (Kyoto, Japan). .. CCK-8 (Catalog #341-07624) and Hoechst 33342 (Catalog #342-07951) were purchased from Dojindo Molecular Technologies, Inc. (Rockville, MD, U.S.A.).

Expressing:

Article Title: Trace-Level Detection of a Niacin Metabolite in Human Blood under Phosphonate Pillar[6]arene-Induced Columnar-Like Aggregation Conditions.
Article Snippet: A rapid method for quantifying the niacin metabolite 1-methylnicotinamide (1-MNA) in human blood is required to address the limitations of existing low-throughput techniques such as mass spectrometry.. In previous studies, we demonstrated that supramolecular pillar[6]arenes (P6As) bearing either carboxylate groups (P6AC) or sulfonate groups (P6AS) functioned as “turn-off” fluorescent sensors for 1-MNA detection in urine by photoinduced electron transfer (PET).. However, because the concentration of 1-MNA in blood is very low, PET-based detection in aqueous solutions was insufficient for reliable quantification on its own.



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