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dtcs  (ChemoMetec)


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    Structured Review

    ChemoMetec dtcs
    Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to <t>induce</t> <t>CEACAM5</t> conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, <t>DTCs,</t> including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.
    Dtcs, supplied by ChemoMetec, used in various techniques. Bioz Stars score: 97/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dtcs/pmc12670079-99-3-10?v=ChemoMetec
    Average 97 stars, based on 642 article reviews
    dtcs - by Bioz Stars, 2026-07
    97/100 stars

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    1) Product Images from "ATOR-4066, a Bispecific Antibody Targeting CD40 and CEACAM5, Induces Strong Myeloid and T Cell–Dependent Tumor Immunity and Synergizes with PD-1 Blockade"

    Article Title: ATOR-4066, a Bispecific Antibody Targeting CD40 and CEACAM5, Induces Strong Myeloid and T Cell–Dependent Tumor Immunity and Synergizes with PD-1 Blockade

    Journal: Cancer Immunology Research

    doi: 10.1158/2326-6066.CIR-25-0075

    Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to induce CEACAM5 conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, DTCs, including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.
    Figure Legend Snippet: Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to induce CEACAM5 conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, DTCs, including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.

    Techniques Used: Functional Assay, Generated, Activation Assay, Irradiation, Expressing, Enzyme-linked Immunosorbent Assay, Reporter Assay, Control, In Vitro, Flow Cytometry



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    Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to <t>induce</t> <t>CEACAM5</t> conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, <t>DTCs,</t> including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.
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    Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to <t>induce</t> <t>CEACAM5</t> conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, <t>DTCs,</t> including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.
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    Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to induce CEACAM5 conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, DTCs, including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.

    Journal: Cancer Immunology Research

    Article Title: ATOR-4066, a Bispecific Antibody Targeting CD40 and CEACAM5, Induces Strong Myeloid and T Cell–Dependent Tumor Immunity and Synergizes with PD-1 Blockade

    doi: 10.1158/2326-6066.CIR-25-0075

    Figure Lengend Snippet: Design and functional evaluation of ATOR-4066. A, Structure of ATOR-4066 generated in the bispecific RUBY format. B, Mode of action of ATOR-4066, as proposed by Hägerbrand and colleagues . C, The ability of serially diluted ATOR-4066 to induce CEACAM5 conditional activation of primary human MoDCs when cocultured for 48 hours with a sublethally UV-irradiated human CEACAM5-expressing cell line (MKN45) was assessed by measuring IL12 secretion in the supernatant by ELISA. Mean ± SD of normalized IL12 levels of nine donors was calculated, and EC 50 values were determined. D, The ability of ATOR-4066 to induce CEACAM5-conditional CD40 agonistic effects in a CD40 reporter assay was assessed in the presence of soluble CEACAM5 compared with a control CD40xCEACAM5 bsAb #3 with a high-affinity CEACAM5 binder targeting the same epitope as ATOR-4066, demonstrating that the CEACAM5 affinity is important. E, DTCs, including tumor-infiltrating CD40 + immune cells and CEACAM5 + tumor cells, obtained from patients with gastric cancer ( n = 6), were stimulated with ATOR-4066 in vitro for 72 hours, and activation markers, CD83 on myeloid cells and B cells, and CD25 on CD4 and CD8 T cells, were assessed using flow cytometry. F, Harvest titer of ATOR-4066 following production in 0.5 L and 1 L shaker flasks and in 2 L bioreactor. *, P ≤ 0.05; ns, not significant.

    Article Snippet: Directly after thawing, DTCs were counted using a NucleoCounter NC-200 (ChemoMetec), stained for CEACAM5 and CD40, and analyzed by flow cytometry to confirm target expression at baseline.

    Techniques: Functional Assay, Generated, Activation Assay, Irradiation, Expressing, Enzyme-linked Immunosorbent Assay, Reporter Assay, Control, In Vitro, Flow Cytometry