Journal: Nature Communications
Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
doi: 10.1038/s41467-026-69129-1
Figure Lengend Snippet: a Experimental scheme of the experiment to monitor dendritic spine morphology of striatal D1 and D2 MSN in the DLS after chemogenetic modulation of the SNc in astrocytic Megf10 conditional knock-down. b Representative confocal z-stack images showing dendritic spines of D1 MSN ( Drd1a-Cre ) and D2 MSN ( Adora2a-Cre ) (White: eGFP, t: thin spine, s: stubby spine, m: mushroom spine). Scale bar, 10 μm. c Quantification of the dendritic spine density in D1 MSN (Scramble; Vehicle: n = 7 mice, CNO: n = 7 mice, sh Megf10 ; Vehicle: n = 8 mice, CNO: n = 8 mice; one-way ANOVA). d Quantification of the dendritic spine density in D2 MSN. (Scramble; Vehicle: n = 6 mice, CNO: n = 7 mice, sh Megf10 ; Vehicle: n = 7 mice, CNO: n = 7 mice; one-way ANOVA). Data were presented as mean values ± SEM.
Article Snippet: Aldh1l1-CreERT2 mice (FVB-Tg( Aldh1l1-cre/ERT2 )1Khakh/J), Drd1 floxed mice (Drd1tm2.1Stl/J), and Drd2 floxed mice (B6.129S4(FVB)-Drd2tm1.1Mrub/J) were purchased from the Jackson Laboratory.
Techniques: Knockdown