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draq7tm  (Biostatus)


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    Biostatus draq7tm
    Draq7tm, supplied by Biostatus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/draq7tm/pm39472557-407-19-20?v=Biostatus
    Average 90 stars, based on 1 article reviews
    draq7tm - by Bioz Stars, 2026-06
    90/100 stars

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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by <t>DRAQ7TM</t> (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched
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    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by DRAQ7TM (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched

    Journal: Epigenetics

    Article Title: Transcriptional signature of cardiac myocyte recovery in mice and human reveals persistent upregulation of epigenetic factors.

    doi: 10.1080/15592294.2025.2506625

    Figure Lengend Snippet: Figure 3. Transcriptome analysis in isolated cardiac myocytes. (a) Workflow for the isolation of cardiac myocytes (CM) for RNAseq analysis from sham, TAC, and rTAC hearts (sham: n = 3, TAC: n = 3, rTAC: n = 3). (b) Sorting strategy for purification of cardiac myocytes. CM were identified by a high forward scattered light (FSC) and side scattered light (SSC) signal. Discrimination of viable and dead CM was achieved by DRAQ7TM (red population). (c) Bar indicates the number of up (red; q < 0.05, fold change (FC) ≥1.3), or downregulated (blue; q < 0.05, FC ≤ 0.77) mRNAs after TAC compared to sham. Pie charts depict enriched GO terms for up or downregulated RNAs (red and blue respectively). (d) Bar shows number of genes that were upregulated after rTAC (upper red part), genes that were upregulated after TAC and rTAC (lower red part), and genes that were regulated in TAC but were not regulated after rTAC (white) as compared to sham. Enriched

    Article Snippet: The pellet was resuspended in 1 ml PBS containing 1 mm EDTA, filtered (30 μm cell strainer, CellTrics, Sysmex) and incubated with DRAQ7TM (Cell Signalling Technology) for 10 min. Cardiac myocyte nuclei were sorted directly into RLT lysis buffer (Qiagen) by flow cytometry (S3eTM, BioRad).

    Techniques: Isolation, Purification