draq5 (Thermo Fisher)

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Thermo Fisher draq5
Draq5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5/nct06114095-25-30-31
Average 90 stars, based on 1 article reviews

draq5 - by Bioz Stars, 2026-09

90/100 stars

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Transfection:

Article Title: A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ph antibody (rabbit) Francis lab 66 N/A Psc antibody (mouse) DSHB Cat# 6E8; RRID: AB_528437 GFP antibody (mouse) DSHB Cat# DSHB-GFP-4C9; RRID: AB_2617422 GFP antibody (mouse) DSHB Cat# DSHB-GFP-12E6; RRID: AB_2617418 GFP antibody (rabbit) Proteintech Cat# 50430-2-AP; RRID: AB_11042881 anti-mouse IgG (rabbit) Abcam Cat# ab171870 Pc antibody (rabbit) Kind gift of J. Mü ller 85 N/A α-Tubulin antibody (mouse) Sigma Aldrich Cat# T5168; RRID: AB_477579 anti-mouse IgG Alexa Fluor 680 conjugated (goat) Thermo Fisher Scientific Cat# A-21057; RRID: AB_141436 anti-rabbit IgG IRDye 800 CW conjugated (goat) LICOR Cat# 925-32211; RRID: AB_2651127 Bacterial and virus strains NEB 5-alpha E. coli NEB Cat# C2987H NEB Turbo E. coli NEB Cat# C2984H DH10Bac E. coli Thermo Fisher Scientific Cat# 10361012 BL21 (DE3) Rosetta pLysS E. coli Sigma Aldrich Cat# 70956-M BL21(DE3) LOBSTR-RIL E. coli Gift from Franç ois Robert lab N/A BL21 Gold (DE3) pLysS E. coli Agilent Cat# 230134 Chemicals, peptides, and recombinant proteins Zeba Spin Desalting Column Thermo Fisher Scientific Cat# 89882 M3 media US Biological Cat# S1013 FBS (for insect cell media) Wisent Cat# 920-045 FBS (for mammalian cell media) Wisent Cat# 080-150 Zeocin Thermo Fisher Scientific Cat# J67140 Blasticidin Wisent Cat# 450-190 Hygromycin Wisent Cat# 400-141 LR Clonase II Thermo Fisher Scientific Cat# 11791020 NEBuilder HiFi DNA Assembly Master Mix NEB Cat# E2621 anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 ATP (lithium salt) Roche Cat# 11140965001 Benzonase Sigma-Aldrich Cat# E1014 Proteinase K BioBasic Cat# PB0451 SYBR Gold Thermo Fisher Scientific Cat# S11494 Cy3 NHS ester Cytiva Cat# 25-9004 Hellmanex III Sigma-Aldrich Cat# Z805939 Sigmacote Sigma-Aldrich Cat# SL2 Pluronic F-127 Sigma-Aldrich Cat# P2443 Glucose Oxidase Sigma-Aldrich Cat# G0543 Catalase Sigma-Aldrich Cat# C3155 TransIT-Insect Transfection Reagent Mirus Cat# 6105 Concanavalin A Medicago Cat# 05-0106 Puromycin Wisent Cat# 400-160 (Continued on next page) e1 Molecular Cell 85, 1–19.e1–e15, June 5, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER KOD Polymerase Millipore Sigma Cat# 71086 TransIT-293 Transfection Reagent Mirus Cat# 2704 Poly-L-Lysine Electron Microscopy Sciences Cat# 19320-A DRAQ5 Thermo Fisher Scientific Cat# 62251 DAPI Sigma Aldrich Cat# D9542 N-propyl gallate Fluka Cat# 02370 TRIzol Reagent Thermo Fisher Scientific Cat# 15596018 RNAse-free water Wisent Cat# 809-115 DNAse I NEB Cat# M0303L Superscript IV Reverse Transcriptase Thermo Fisher Scientific Cat# 18090050 Deoxynucleotide (dNTP) Mix NEB Cat# N0447 RNAse Inhibitor, Murine NEB Cat# M0314 RNAse A BioShop Cat# RNA675 NTB Binding buffer Macherey-Nagel Cat# 740595 Dynabeads Protein G Thermo Fisher Scientific Cat# 10004D Bovine Serum Albumine (BSA) BioShop Cat# ALB001 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25741 MS-grade water Thermo Fisher Scientific Cat# W6-4 Critical commercial assays QuickChange Lightning Site-Directed Mutagenesis Kit Agilent Cat# 210518 RNA Clean & Concentrator-5 Kit Zymo Research Cat# R1013 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat# 740609 Deposited data Mass spectrometry data This study PRIDE: PXD060324 Experimental models: Cell lines Sf9 cells Expression Systems Cat# 94-001F Kc167 cells Gift from François Robert lab N/A Flp-IN T-REx 293 cells Gift from Jean-Franç ois Cô té lab N/A Experimental models: Organisms/strains D. melanogaster: hh-Gal4/tub-Gal80ts: y[1],w[*]; P{w[+mC], hh-Gal4}, P{w[+mC]=tubP-GAL80ts}2/TM6B,Tb[1] Gift from David Hipfner lab N/A D. melanogaster: H2Av-RFP: w[*]; P{w[+mC]=His2Av-mRFP1}II.2 Gift from David Hipfner lab Stock# 23651; RRID: BDSC_23651 D. melanogaster: UAS-GFP-nls: w[1118]; P{w[+mC]=UAS-GFP.nls}8 Gift from David Hipfner lab Stock# 4776; RRID: BDSC_4776 D. melanogaster: UAS-Venus-Ph WT : w[1118];y[+],{w[+mC]UASVenus-Ph WT }attP2/TM3, Sb1, e 1 This study N/A D. melanogaster: UAS-Venus-Ph PHC3L : w[1118];y[+],{w[+mC], UAS-Venus-Ph PHC3L }attP2/TM3, Sb 1 , e 1 This study N/A D. melanogaster: UAS-Venus-Ph SAMsurf : w[1118];y[+],{w[+mC], UAS-Venus-Ph SAMsurf }attP2/TM3, Sb 1 , e 1 This study N/A Oligonucleotides Random hexamers IDT Cat# 51-01-18-26 Primer and DNA sequences are listed in Table S6 This study N/A Recombinant DNA Gateway donor Seif et al. 31 N/A pMTVBH This study N/A (Continued on next page) Molecular Cell 85, 1–19.e1–e15, June 5, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDEST-VF This study N/A pAc5-H2Av-RFP-Blast This study N/A pFBFGv2 mini-Ph variants This study N/A pET-mini-Ph EH Seif et al.31 N/A pET-mini-Ph PHC3L-EH/SAMsurf-EH This study N/A pET-HisSUMO-hGSDMD Kambara et al. 86 Addgene #111559; RRID: Addgene_111559 pET-HisSUMO-Linker-SAM variants This study N/A pET-Linker-SAM variants (for AUC) This study N/A pAc5-sgRNA-Cas9 Bassett et al. 87 Addgene #49330; RRID: Addgene_49330 pCRISPaint-TagRFP-T2A-PuroR Schmid-Burgk et al. 57 Addgene #80971; RRID: Addgene_80971 pCRISPaint Ph iV templates This study N/A pUASt-attB Gift from David Hipfner lab N/A pFGET19-Ulp1 Guerrero et al. 88 Addgene #64697; RRID: Addgene_64697 pCFD3-Frame selector 1 Bosch et al. 58 Addgene #127554; RRID: Addgene_127554 pOG44 Gift from Jean-Franç ois Cô té lab N/A Software and algorithms ASTRA (v6.1.6.5) Wyatt Technology N/A AcquireMP and DiscoverMP Refeyn N/A FlowJo (v10.7.2) BD N/A QuantStudio Design&Analysis (v1.5.3) Thermo Fisher Scientific N/A Zen 2012 Zeiss N/A Fiji (ImageJ v1.54g) Schindelin et al. 89 N/A ImageQuant TL (v8.1.0.0) Cytiva N/A GraphPad Prism 10 (v10.3.0) Dotmatics N/A MaxQuant (v2.4.0.0) Cox and Mann 90 N/A CellProfiler (v4.2.5) Stirling et al. 91 N/A Zen 2 (Blue Edition) Zeiss N/A Illastik (v1.3.3) Berg et al. 92 N/A Adobe Illustrator 2023 (v27.5) Adobe N/A Excel (v2412) Microsoft N/A Adobe InDesign 2025 (v20.0.1) Adobe N/A Other Amicon 10 kDa MWCO Concentrator Millipore Cat# UFC5010BK HisTrap-HP Column (1 ml) Cytiva Cat# 29051021 HiTrapQ-HP Column (1 ml) Cytiva Cat# 29051325 HisTrap-HP Column (5 ml) Cytiva Cat# 17524801 HiTrapQ-HP Column (5 ml) Cytiva Cat# 17115401 Superdex 200 10/300 GL Cytiva Cat# 28990944 HiTrap SP HP (5 ml) Cytiva Cat# 17115201 Superdex 75 26/600 Cytiva Cat# 28989334 384-well imaging plate (SensoPlate) Greiner Bio-One Cat# 781892 (Continued on next page) e3 Molecular Cell 85, 1–19.e1–e15, June 5, 2025

Electron Microscopy:

Article Title: High-Resolution Correlative Microscopy Approach for Nanobio Interface Studies of Nanoparticle-Induced Lung Epithelial Cell Damage.
Article Snippet: .. The murine epithelial lung tissue cell line (LA-4, ATCC CCL-196), murine alveolar lung macrophage cell line (MH-S; ATCC, CRL-2019), F-12K medium (Gibco), fetal bovine serum (ATCC), 1% penicillin-streptomycin (Sigma), 1% nonessential amino acids, Lglutamine, beta-mercaptoethanol (Gibco), phosphate buffer saline (PBS), live cell imaging solution (LCIS, Invitrogen); titanium dioxide nanotubes (TiO2 NTs) in anatase form synthesized in-house; 95 μ-Slide 8-well (Ibidi), silicon nitride Si3N4 support film (PELCO, 21509CL, Ted Pella), TEM formvar/carbon film on Au Gilder 200 F1 finder grids (FCF200F1-AU-50, EMS), ammonium acetate (Sigma-Aldrich), PTFE coated high precision and ultrafine tweezers (72919-3SATe, EMS), propane transfer system for plunge freezer (37015, Electron Microscopy Sciences); AlexaFluor 647 (Thermo Fischer Scientific), Atto 594 (ATTO-TEC), CellMask Orange (Invitrogen), SiR-actin (Spirochrome), Draq5 (Invitrogen), MitoTracker Orange CMTMRos (Invitrogen), PSM-39 (in-house). ..

Reverse Transcription:

Binding Assay:

SYBR Green Assay:

Mutagenesis:

Polymerase Chain Reaction:

Mass Spectrometry:

Recombinant:

Staining:

Article Title: Mammalian mitochondrial translation infidelity leads to oxidative stress-induced cell cycle arrest and cardiomyopathy
Article Snippet: .. The cardiomyocytes were stained with DRAQ5 (Thermo) and then the nuclei were counted, with approximately 2× 103 cardiomyocytes counted per sample and 3-4 independent samples obtained per group. ..

Article Title: A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization.
Article Snippet: For the Poly-L-lysine coating, the glass surface was treated like for ConA coating but by adding 0.1% (w/v) aqueous Poly-L-lysine solution (Electron Microscopy Sciences). .. Protein expression was induced for one day in standard media with 1 μg/ml Doxycycline but without selection antibiotics before image acquisition, for which nuclei were stained by addition of DRAQ5 (Thermo Fisher Scientific) to a final concentration of 2.5 μM (from a 125 μM dilution in PBS). ..

Expressing:

Selection:

Concentration Assay:

Saline:

Live Cell Imaging:

Titanium Dioxide:

Synthesized:

Transmission Electron Microscopy:

Marker:

Article Title: PARG Mutation Uncovers Critical Structural Determinant for Poly(ADP-Ribose) Hydrolysis and Chromatin Regulation in Embryonic Stem Cells
Article Snippet: The corresponding secondary antibodies used were anti-mouse Alexa 488 (1:1000, A28175, Invitrogen) and anti-rabbit Alexa 568 (1:1000, A-11011, Invitrogen) for 45 min at RT. .. Draq5 (Thermo Fisher) or TOTO3 (Biotium, Fremont, CA, USA) was used as a DNA marker. .. Confocal imaging was performed using a Leica DMi8 microscope.

other:

Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout
Article Snippet: DRAQ5 , Thermo Scientific , Cat# 52251.

Centrifugation:

Article Title: Single Cell Sequencing and Multidimensional Omics Studies in Cardiovascular and Neurological Diseases
Article Snippet: .. 4 Sorting: Centrifugation was repeated under the above conditions, the supernatant was removed, and the cell particles were re-suspended in 300 μl cell suspension buffer (0.04%BSA, 1×PBS) and 1 μl DRAQ5 (Thermo Fisher Scientific, 62251) and 4, 6-diaminidine - 2-phenylindole (DAPI; BD Biosciences, 564907) and incubate for 5 minutes before sorting. ..

Review