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draq5  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc draq5
    Draq5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/draq5/pm41933263-63-8-10
    Average 86 stars, based on 1 article reviews
    draq5 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: 1-Deoxysphingolipids cause autophagosome and lysosome accumulation and trigger NLRP3 inflammasome activation.
    Article Snippet: .. Cells were fixed and nuclei were stained using PBS (Sigma, 806552) containing 4% paraformaldehyde and DRAQ5 (Cell Signaling Technology, 4084) before sample washing and mounting. .. Semi-automated epifluorescence microscopy was performed on a Zeiss observer Z1 microscope using a 20x objective and the filter sets 47 and 50 from Zeiss.

    Article Title: Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed
    Article Snippet: .. The nuclear stain DRAQ5 (Cell Signaling Technology, Cat. No. 4084) imaging was carried out using λex = 633 nm and λem = 650−735 nm. .. Briefly, 2 × 104 MCF7 cells (ATCC, Manassas, VA) were seeded in 200 μL to an μ-Slide 8 Well (IBIDI GmbH, Graf̈elfing, Bayern, Germany) and cultivated for 24 h. Then 600 μg/mL of QD was added and in 5 h were cells stained by the 5 μM DNA intercalating stain DRAQ5 (Cell Signaling, Danvers, MA) for at least 5 min. 2.10.

    Article Title: Golgi Phosphoprotein 4 (Gpp130) Is A Sensitive And Selective Cellular Target Of Manganese Exposure
    Article Snippet: Tissues were then washed with PBS, phosphate buffered saline Tween (PBST), and incubated with secondary antibody (goat 7 anti-rabbit IgG, Alexa Fluor 488; Molecular Probes). .. Slides were washed again with PBST and stained for 10 min with Draq5 (4084; Cell Signaling Technology, Beverly, MA), followed by a final washing with PBS. .. Slides were then loaded with Fluoromount GTM (Southern Biotech) and cover-slipped prior to analyses by confocal microscopy.

    Imaging:

    Article Title: Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed
    Article Snippet: .. The nuclear stain DRAQ5 (Cell Signaling Technology, Cat. No. 4084) imaging was carried out using λex = 633 nm and λem = 650−735 nm. .. Briefly, 2 × 104 MCF7 cells (ATCC, Manassas, VA) were seeded in 200 μL to an μ-Slide 8 Well (IBIDI GmbH, Graf̈elfing, Bayern, Germany) and cultivated for 24 h. Then 600 μg/mL of QD was added and in 5 h were cells stained by the 5 μM DNA intercalating stain DRAQ5 (Cell Signaling, Danvers, MA) for at least 5 min. 2.10.



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    draq5 staining solution  (Miltenyi Biotec)
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    Miltenyi Biotec draq5 staining solution
    (A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with <t>Draq5</t> (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.
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    https://www.bioz.com/product/draq5/DRAQ5+Staining+Solution/bio_rxiv__64898__2026__02__16__706237-241-14-17
    Average 97 stars, based on 1 article reviews
    draq5 staining solution - by Bioz Stars, 2026-09
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    Image Search Results


    (A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with Draq5 (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.

    Journal: bioRxiv

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function

    doi: 10.64898/2026.02.16.706237

    Figure Lengend Snippet: (A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with Draq5 (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.

    Article Snippet: Filipin was removed, and cells were washed and imaged in PBS containing 5 μM Draq5 staining solution (Miltenyi Biotec) using a Zeiss Elyra PS.1 confocal microscope (Zeiss) equipped with a 63X/1.4 oil DIC objective.

    Techniques: Derivative Assay, RNA Sequencing, Gene Expression, Single Cell, Expressing, Staining, Isolation