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wild type l-dpo4  (Trevigen)

 
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    Trevigen wild type l-dpo4
    Wild Type L Dpo4, supplied by Trevigen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dpo4/dpo4/us12116627-300-6-16
    Average 90 stars, based on 1 article reviews
    wild type l-dpo4 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    DNA Synthesis:

    Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases
    Article Snippet: DNA polymerase δ [ ], human DNA polymerase η [ ], and Saccharomyces cerevisiae DNA polymerase ε [ ] were purified as described previously. .. Dpo4 was purchased from Trevigen (Gaithersburg, USA).

    Article Title: Measuring cation dependent DNA polymerase fidelity landscapes by deep sequencing.
    Article Snippet: and N1.2.2 (59-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-39) to a final concentration of 300 nM each and annealing them via the same protocol. .. Primer extensions were performed as per the manufacturer’s instructions (Dpo4, Klenow exo2, Phusion) in 10 mL reactions containing 1 mL annealing reaction, 50 mM each dNTP, and 1 mL of a 1:1000 dilution of Dpo4 (Trevigen) in Dpo4 annealing buffer, 1 mL Klenow exo2, or 5 mL 26Phusion Mastermix in HF buffer, in 16extension buffer (Table S3). .. Primer extensions were initiated with the addition of divalent cation (chloride salt) to the reaction mixture and incubation at 37uC for 1 h, except f

    Article Title: Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing
    Article Snippet: and N1.2.2 ( 5′-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3′ ) to a final concentration of 300 nM each and annealing them via the same protocol. .. Primer extensions were performed as per the manufacturer's instructions (Dpo4, Klenow exo − , Phusion) in 10 μL reactions containing 1 μL annealing reaction, 50 μM each dNTP, and 1 μL of a 1∶1000 dilution of Dpo4 (Trevigen) in Dpo4 annealing buffer, 1 μL Klenow exo − , or 5 μL 2× Phusion Mastermix in HF buffer, in 1× extension buffer ( ). .. Primer extensions were initiated with the addition of divalent cation (chloride salt) to the reaction mixture and incubation at 37°C for 1 h, except f

    Article Title: Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ?
    Article Snippet: template was slowly cooled to room temperature. .. Primer-extension assays with Dpo4 (Trevigen) were performed in 10 m m HEPES-NaOH, pH 7.8, 1 m m dithiothreitol, 50 m m NaCl, 100 μg/ml bovine serum albumin, 0.1% Triton X-100, 10 m m MgCl 2 , 100 μ m dNTP, and 2 n m primer-template (Seq1–58 nt) at 70 °C. .. Analysis of Translesion Synthesis Products from Pri

    Incubation:

    Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases
    Article Snippet: DNA polymerase δ [ ], human DNA polymerase η [ ], and Saccharomyces cerevisiae DNA polymerase ε [ ] were purified as described previously. .. Dpo4 was purchased from Trevigen (Gaithersburg, USA).

    Article Title: Measuring cation dependent DNA polymerase fidelity landscapes by deep sequencing.
    Article Snippet: and N1.2.2 (59-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-39) to a final concentration of 300 nM each and annealing them via the same protocol. .. Primer extensions were performed as per the manufacturer’s instructions (Dpo4, Klenow exo2, Phusion) in 10 mL reactions containing 1 mL annealing reaction, 50 mM each dNTP, and 1 mL of a 1:1000 dilution of Dpo4 (Trevigen) in Dpo4 annealing buffer, 1 mL Klenow exo2, or 5 mL 26Phusion Mastermix in HF buffer, in 16extension buffer (Table S3). .. Primer extensions were initiated with the addition of divalent cation (chloride salt) to the reaction mixture and incubation at 37uC for 1 h, except f

    Article Title: Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing
    Article Snippet: and N1.2.2 ( 5′-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3′ ) to a final concentration of 300 nM each and annealing them via the same protocol. .. Primer extensions were performed as per the manufacturer's instructions (Dpo4, Klenow exo − , Phusion) in 10 μL reactions containing 1 μL annealing reaction, 50 μM each dNTP, and 1 μL of a 1∶1000 dilution of Dpo4 (Trevigen) in Dpo4 annealing buffer, 1 μL Klenow exo − , or 5 μL 2× Phusion Mastermix in HF buffer, in 1× extension buffer ( ). .. Primer extensions were initiated with the addition of divalent cation (chloride salt) to the reaction mixture and incubation at 37°C for 1 h, except f

    Article Title: Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ?
    Article Snippet: template was slowly cooled to room temperature. .. Primer-extension assays with Dpo4 (Trevigen) were performed in 10 m m HEPES-NaOH, pH 7.8, 1 m m dithiothreitol, 50 m m NaCl, 100 μg/ml bovine serum albumin, 0.1% Triton X-100, 10 m m MgCl 2 , 100 μ m dNTP, and 2 n m primer-template (Seq1–58 nt) at 70 °C. .. Analysis of Translesion Synthesis Products from Pri

    Concentration Assay:

    Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases
    Article Snippet: DNA polymerase δ [ ], human DNA polymerase η [ ], and Saccharomyces cerevisiae DNA polymerase ε [ ] were purified as described previously. .. Dpo4 was purchased from Trevigen (Gaithersburg, USA).

    Article Title: Measuring cation dependent DNA polymerase fidelity landscapes by deep sequencing.
    Article Snippet: and N1.2.2 (59-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-39) to a final concentration of 300 nM each and annealing them via the same protocol. .. Primer extensions were performed as per the manufacturer’s instructions (Dpo4, Klenow exo2, Phusion) in 10 mL reactions containing 1 mL annealing reaction, 50 mM each dNTP, and 1 mL of a 1:1000 dilution of Dpo4 (Trevigen) in Dpo4 annealing buffer, 1 mL Klenow exo2, or 5 mL 26Phusion Mastermix in HF buffer, in 16extension buffer (Table S3). .. Primer extensions were initiated with the addition of divalent cation (chloride salt) to the reaction mixture and incubation at 37uC for 1 h, except f

    Article Title: Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing
    Article Snippet: and N1.2.2 ( 5′-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3′ ) to a final concentration of 300 nM each and annealing them via the same protocol. .. Primer extensions were performed as per the manufacturer's instructions (Dpo4, Klenow exo − , Phusion) in 10 μL reactions containing 1 μL annealing reaction, 50 μM each dNTP, and 1 μL of a 1∶1000 dilution of Dpo4 (Trevigen) in Dpo4 annealing buffer, 1 μL Klenow exo − , or 5 μL 2× Phusion Mastermix in HF buffer, in 1× extension buffer ( ). .. Primer extensions were initiated with the addition of divalent cation (chloride salt) to the reaction mixture and incubation at 37°C for 1 h, except f

    Article Title: Translesion Synthesis of Abasic Sites by Yeast DNA Polymerase ?
    Article Snippet: template was slowly cooled to room temperature. .. Primer-extension assays with Dpo4 (Trevigen) were performed in 10 m m HEPES-NaOH, pH 7.8, 1 m m dithiothreitol, 50 m m NaCl, 100 μg/ml bovine serum albumin, 0.1% Triton X-100, 10 m m MgCl 2 , 100 μ m dNTP, and 2 n m primer-template (Seq1–58 nt) at 70 °C. .. Analysis of Translesion Synthesis Products from Pri



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    Image Search Results


    Experimental design and adaptation of tXR-seq for mapping nucleotide excision repair of AFB1–dG adducts. ( A ) Overview of the experimental design for in vivo excision assay, CPD/(6–4)PP XR-seq (4 h post-exposure) and AFB1–dG tXR-seq (4 h post-exposure). ( B ) Detection of excision products by excision assay after UVC (20 J/m 2 ) or AFB1 (40 μM) treatment in HepG2 cells. Excision products from one-quarter of a 150 mm petri dish of UVC-treated HepG2 cells (0.5 h post-exposure) were loaded into the gel, while for AFB1-treated HepG2 cells (4 h post-exposure), excision products from two 150 mm petri dishes were used in the excision assay. M, DNA size marker; C, non-damaged control. ( C ) Sequencing library construction workflow for CPD/(6–4)PP XR-seq and AFB1–dG tXR-seq. Red asterisk indicates DNA bulky adduct. Excision products, released during nucleotide excision repair, are precipitated with TFIIH/XPG antibodies, extracted and ligated to adaptors. Then the adaptor-containing oligomers are precipitated with damage IP. Photolyases are used for the reversal of UV damage for CPD/(6–4)PP XR-seq. For AFB1–dG tXR-seq, Dpo4 (pink) is the sulfolobus DNA polymerase IV, a Y-family DNA polymerase known for its ability to bypass various DNA lesions. It is used for bypassing the AFB1–dG damage during the primer extension before the PCR amplification. ( D ) Optimization of AFB1–dG tXR-seq library preparation. Lanes show results using different anti-AFB1 antibodies (6A10 and AFA-1) and translesion synthesis DNA polymerases (Dpo4 and polymerase ζ). Lanes 1, 2, and 7 are non-damaged controls. Libraries were analyzed by 10% native polyacrylamide gel electrophoresis. Red star marks PCR products containing inserts; black arrow indicates adapter dimers.

    Journal: Nucleic Acids Research

    Article Title: Nucleotide excision repair of aflatoxin-induced DNA damage within the 3D human genome organization

    doi: 10.1093/nar/gkae755

    Figure Lengend Snippet: Experimental design and adaptation of tXR-seq for mapping nucleotide excision repair of AFB1–dG adducts. ( A ) Overview of the experimental design for in vivo excision assay, CPD/(6–4)PP XR-seq (4 h post-exposure) and AFB1–dG tXR-seq (4 h post-exposure). ( B ) Detection of excision products by excision assay after UVC (20 J/m 2 ) or AFB1 (40 μM) treatment in HepG2 cells. Excision products from one-quarter of a 150 mm petri dish of UVC-treated HepG2 cells (0.5 h post-exposure) were loaded into the gel, while for AFB1-treated HepG2 cells (4 h post-exposure), excision products from two 150 mm petri dishes were used in the excision assay. M, DNA size marker; C, non-damaged control. ( C ) Sequencing library construction workflow for CPD/(6–4)PP XR-seq and AFB1–dG tXR-seq. Red asterisk indicates DNA bulky adduct. Excision products, released during nucleotide excision repair, are precipitated with TFIIH/XPG antibodies, extracted and ligated to adaptors. Then the adaptor-containing oligomers are precipitated with damage IP. Photolyases are used for the reversal of UV damage for CPD/(6–4)PP XR-seq. For AFB1–dG tXR-seq, Dpo4 (pink) is the sulfolobus DNA polymerase IV, a Y-family DNA polymerase known for its ability to bypass various DNA lesions. It is used for bypassing the AFB1–dG damage during the primer extension before the PCR amplification. ( D ) Optimization of AFB1–dG tXR-seq library preparation. Lanes show results using different anti-AFB1 antibodies (6A10 and AFA-1) and translesion synthesis DNA polymerases (Dpo4 and polymerase ζ). Lanes 1, 2, and 7 are non-damaged controls. Libraries were analyzed by 10% native polyacrylamide gel electrophoresis. Red star marks PCR products containing inserts; black arrow indicates adapter dimers.

    Article Snippet: Sulfolobus DNA polymerase IV (Dpo4) (Cat. No. M0327) and DNA polymerase ζ (Cat. No. 51) were purchased from NEB and Enzymax, respectively.

    Techniques: In Vivo, Excision Assay, Marker, Control, Sequencing, Amplification, Translesion Synthesis, Polyacrylamide Gel Electrophoresis

    DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase ζ (panel A), DNA polymerase η (panel B) and Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) (panel C). DNA polymerase ζ (1.7 ng), DNA polymerase η (0.4 ng) and Dpo4 (0.4 ng) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls

    Journal: Genes and Environment

    Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

    doi: 10.1186/s41021-022-00236-3

    Figure Lengend Snippet: DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase ζ (panel A), DNA polymerase η (panel B) and Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) (panel C). DNA polymerase ζ (1.7 ng), DNA polymerase η (0.4 ng) and Dpo4 (0.4 ng) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls

    Article Snippet: Dpo4 was purchased from Trevigen (Gaithersburg, USA).

    Techniques: DNA Synthesis, Incubation