rabbit anti dock7  (Proteintech)

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: (A) Schematic of the Dock7 wild type (+) and Dock7 exons 3–4 deletion ( Dock7-em2) transgenic allele. Image created with BioRender.com. (B) Predicted translation of the Dock7+ and Dock7-em2 alleles. Image created with BioRender.com. (C) Representative images of Dock7 +/+ , Dock7 +/em2 , and Dock7 em2/em2 mice.

Article Snippet: Dock7 exons 3–4 levels were quantified using a TaqMan gene expression assay containing primers that span across Dock7 exons 3–4 (Thermo Fisher, Mm01259842_g1, 4351372) and TaqMan Fast Advanced Master Mix (Thermo Fisher, 4444556).

Techniques: Transgenic Assay

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: (A) RNA expression of Dock7 exons 3–4, exons 11–12, and exons 34–35 was analyzed in BMSCs differentiated for 7 days in osteogenic media. Points represent individual replicates for all 3 experiments. (B) Quantitative protein analysis of DOCK7 peptides was performed by mass spectrometry in BMSC isolated from Dock7 +/+ and Dock7 em2/em2 mice. N=2 mice/group. Points represent replicates from all mice. (C) DOCK7 peptides were detected by mass spectrometry and used to quantify total DOCK7 levels. High confidence (green), medium confidence (yellow), and low confidence (red) DOCK7 peptides are indicated. The protein sequence corresponding to DOCK7 exons 3–4, the DHR1 domain, and the DHR2 domain is underlined and labeled.

Article Snippet: Dock7 exons 3–4 levels were quantified using a TaqMan gene expression assay containing primers that span across Dock7 exons 3–4 (Thermo Fisher, Mm01259842_g1, 4351372) and TaqMan Fast Advanced Master Mix (Thermo Fisher, 4444556).

Techniques: RNA Expression, Mass Spectrometry, Isolation, Sequencing, Labeling

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet:

Article Snippet: Dock7 exons 3–4 levels were quantified using a TaqMan gene expression assay containing primers that span across Dock7 exons 3–4 (Thermo Fisher, Mm01259842_g1, 4351372) and TaqMan Fast Advanced Master Mix (Thermo Fisher, 4444556).

Techniques:

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: Body composition was measured by DXA analysis in male and female Dock7 em2/em2 mice and compared to Dock7 +/+ control mice. Data was analyzed by 2-way ANOVA and Holm-Šídák’s test for post-hoc analysis. N=14–15 mice/group. Points represent each mouse.

Article Snippet: Dock7 exons 3–4 levels were quantified using a TaqMan gene expression assay containing primers that span across Dock7 exons 3–4 (Thermo Fisher, Mm01259842_g1, 4351372) and TaqMan Fast Advanced Master Mix (Thermo Fisher, 4444556).

Techniques: Control

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: Osteogenic differentiation was assessed in BMSCs that were isolated from Dock7 +/+ and Dock7 em2/em2 mice after 7 days of osteogenic differentiation. (A) Representative image of von Kossa and alkaline phosphatase staining. (B) Expression of the osteoblast-related genes Runx2 , AlpI , and Bglap . Points represent individual replicates for all 3 experiments.

Article Snippet: Dock7 exons 3–4 levels were quantified using a TaqMan gene expression assay containing primers that span across Dock7 exons 3–4 (Thermo Fisher, Mm01259842_g1, 4351372) and TaqMan Fast Advanced Master Mix (Thermo Fisher, 4444556).

Techniques: Isolation, Staining, Expressing

Journal: bioRxiv

Article Title: Mysm1 mutations in meander tail mice cause anterior-selective cerebellum malformation

doi: 10.64898/2026.02.25.708017

Figure Lengend Snippet: ( A ) Reference (Ref) and 23-bp deletion allele (D32Efs) recovered at exon 2. RNA guide sequence is underlined, with protospacer-adjacent motif shaded. Arrowhead, predicted cleavage site. Lowercase intron, uppercase exon sequences. Asterisk, T>A in mea J . ( B ) Compound mea J /Δ96 heterozygotes had shorter, kinked tails with variegated pigmentation characteristic of mea . ( C ) Classical mea alleles (from stocks carrying closely-linked Lepr db or Dock7 m mutation) and compound heterozygotes between mea J and either mea 2J or D32Efs all showed reductions in white blood cells (WBC), neutrophils (NE), lymphocytes (LY), and red blood cells (RBC), but elevated mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH), consistent with the bone marrow failure reported for Mysm1 knockout mice. Mutants in orange, sex-matched littermate controls navy. ( D ) Midline sagittal images illustrate selective reduction of the anterior compartment (folia I-VIa, grey mask) in mea 2J , D32Efs, and compound heterozygous mutations relative to littermate controls. ( E ) Measured areas at midline showed dramatic reductions of the anterior and slight reductions in the posterior cerebellum relative to sex-matched littermates in both females (orange) and males (cyan).

Article Snippet: BKS.Cg– Dock7 m + / + Lepr db (Stock #000700), BKS.Cg– mea J Lepr db / + + (Stock #001192), and BKS.Cg– mea 2J Dock7 m / + + (Stock #001049) were obtained from live colonies and cryopreserved stocks at The Jackson Laboratories, as were mapping partner strains CAST/EiJ (Stock #000928), A/J (Stock #000646), and FVB/NJ (Stock #001800).

Techniques: Sequencing, Mutagenesis, Knock-Out

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: (A) Schematic of the Dock7 wild type (+) and Dock7 exons 3–4 deletion ( Dock7-em2) transgenic allele. Image created with BioRender.com. (B) Predicted translation of the Dock7+ and Dock7-em2 alleles. Image created with BioRender.com. (C) Representative images of Dock7 +/+ , Dock7 +/em2 , and Dock7 em2/em2 mice.

Article Snippet: Briefly, the Dock7-em2 Cjr ( Dock7-em2 ) allele was generated by crossing the C57BL/6J-Dock7 em1/em1 mice with the B6.Cg- Edil3 Tg(Sox2-cre)1Amc /J (Sox2-cre, The Jackson Laboratory, 8454) line in the laboratory of Clifford Rosen at MHIR.

Techniques: Transgenic Assay

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: (A) RNA expression of Dock7 exons 3–4, exons 11–12, and exons 34–35 was analyzed in BMSCs differentiated for 7 days in osteogenic media. Points represent individual replicates for all 3 experiments. (B) Quantitative protein analysis of DOCK7 peptides was performed by mass spectrometry in BMSC isolated from Dock7 +/+ and Dock7 em2/em2 mice. N=2 mice/group. Points represent replicates from all mice. (C) DOCK7 peptides were detected by mass spectrometry and used to quantify total DOCK7 levels. High confidence (green), medium confidence (yellow), and low confidence (red) DOCK7 peptides are indicated. The protein sequence corresponding to DOCK7 exons 3–4, the DHR1 domain, and the DHR2 domain is underlined and labeled.

Article Snippet: Briefly, the Dock7-em2 Cjr ( Dock7-em2 ) allele was generated by crossing the C57BL/6J-Dock7 em1/em1 mice with the B6.Cg- Edil3 Tg(Sox2-cre)1Amc /J (Sox2-cre, The Jackson Laboratory, 8454) line in the laboratory of Clifford Rosen at MHIR.

Techniques: RNA Expression, Mass Spectrometry, Isolation, Sequencing, Labeling

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: Body composition was measured by DXA analysis in male and female Dock7 em2/em2 mice and compared to Dock7 +/+ control mice. Data was analyzed by 2-way ANOVA and Holm-Šídák’s test for post-hoc analysis. N=14–15 mice/group. Points represent each mouse.

Article Snippet: Briefly, the Dock7-em2 Cjr ( Dock7-em2 ) allele was generated by crossing the C57BL/6J-Dock7 em1/em1 mice with the B6.Cg- Edil3 Tg(Sox2-cre)1Amc /J (Sox2-cre, The Jackson Laboratory, 8454) line in the laboratory of Clifford Rosen at MHIR.

Techniques: Control

Journal: bioRxiv

Article Title: Deletion of Dock7 Exons 3 and 4 Results in Reduced Trabecular Microarchitecture and a Decrease in Mineralization

doi: 10.64898/2025.12.31.696872

Figure Lengend Snippet: Osteogenic differentiation was assessed in BMSCs that were isolated from Dock7 +/+ and Dock7 em2/em2 mice after 7 days of osteogenic differentiation. (A) Representative image of von Kossa and alkaline phosphatase staining. (B) Expression of the osteoblast-related genes Runx2 , AlpI , and Bglap . Points represent individual replicates for all 3 experiments.

Article Snippet: Briefly, the Dock7-em2 Cjr ( Dock7-em2 ) allele was generated by crossing the C57BL/6J-Dock7 em1/em1 mice with the B6.Cg- Edil3 Tg(Sox2-cre)1Amc /J (Sox2-cre, The Jackson Laboratory, 8454) line in the laboratory of Clifford Rosen at MHIR.

Techniques: Isolation, Staining, Expressing