dmbt1 (Proteintech)
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Structured Review
Proteintech
dmbt1
Dmbt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmbt1/DMBT1+Antibody/pmc12343785-246-17-19
Average 93 stars, based on 4 article reviews
Dmbt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmbt1/DMBT1+Antibody/pmc12343785-246-17-19
Average 93 stars, based on 4 article reviews
dmbt1 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Breast cancer induces CD62L + Kupffer cells via DMBT1 to promote neutrophil extracellular trap formation and liver metastasis"
Article Title: Breast cancer induces CD62L + Kupffer cells via DMBT1 to promote neutrophil extracellular trap formation and liver metastasis
Journal: Cell Discovery
doi: 10.1038/s41421-025-00819-8
Figure Legend Snippet: a Heatmap of secreted protein-encoding genes differentially expressed in MDA-MB-231 vs LvM16. b Overall survival of breast cancer patients stratified by DMBT1 expression in the UNC cohort ( GSE3521 , GSE26338 ). c – e DMBT1 expression in human breast cancer of the Qilu cohort. Shown are tumoral DMBT1 mRNA levels ( c ) and serological DMBT1 protein levels ( d ) of those with liver-metastatic or non-metastatic primary tumors, and liver metastasis-free survival of patients stratified by tumoral DMBT1 expression ( e ). f DMBT1 mRNA and protein expression in MDA-MB-231 sublines. g – i CD62L induction ( g , h ) and Ccl8 expression ( i ) of KCs treated with CM of the indicated tumor cells with or without DMBT1 overexpression or knockdown. j NETosis of neutrophils cultured with CM from the KCs in i . n = 220 ( b ), 65 ( c , e ) and 22 ( d ) patients, and 3 biological repeats ( f – j ). P values were obtained by log-rank test ( b , e ) or two-tailed unpaired t -test (others). Data are shown as mean ± SD.
Techniques Used: Expressing, Over Expression, Knockdown, Cell Culture, Two Tailed Test
Figure Legend Snippet: a – c In vivo BLI ( a ), ex vivo BLI ( b ) and NETosis ( c ) in livers of NOD-SCID mice treated with Clod after intrasplenic inoculation of MCF7 with or without DMBT1 overexpression. d–f In vivo BLI ( d ), ex vivo BLI ( e ) and NETosis ( f ) of NOD-SCID mice treated with GSK484 after intrasplenic inoculation of MCF7 with or without DMBT1 overexpression. g – j NETosis ( g ), CD62L + KCs ( h ) and in vivo BLI ( i ) of livers, and survival ( j ) of nude mice after intrasplenic injection of LvM16 cells with DMBT1 knockdown. k , l Primary tumor growth ( k ) and liver metastasis ( l ) in C57BL/6 mice after orthotopic injection of AT3 cells with Dmbt1 knockdown. n = 3 or 4 ( a , b ), 3 ( c , h , k , l ), 7 ( d , i , j ), 4 ( e , f , g ) mice per group. P values were obtained by repeated measures two-way ANOVA ( a , d , i , k ), Log rank test ( j ) or two-tailed unpaired t -test (others). Scale bars, 50 μm. Data are shown as mean ± SEM ( a, d, i, k ) or mean ± SD (others).
Techniques Used: In Vivo, Ex Vivo, Over Expression, Injection, Knockdown, Two Tailed Test
Figure Legend Snippet: a Co-IP assays for DMBT1 and MUC1-C in 293 T and HL60 cells. Ctrl, Ni-NTA bead control sample. b p65 phosphorylation and nuclear distribution of MUC1-C in KCs after treatment of CM of cancer cells with or without DMBT1 knockdown/overexpression. c ChIP-qPCR analysis of p65 binding to Ccl8 or Cd62l promoters in monocytes. d p65 phosphorylation and CCL8 expression of KCs cultured with DMBT1 -overexpressing MCF7 CM and/or JSH-23 (30 μM). e – g CD62L induction ( e ), Ccl8 and Cd62l expression ( f ) of KCs after treatment of LvM16 CM, with or without GO203, and the NET-inducing effects of the CM of such KCs ( g ). h – m GO203 treatment (15 mg/kg) of NOD-SCID mice with intrasplenic injection of MCF7 cells with DMBT1 overexpression. Shown are flow cytometry analysis of CD62L + KCs in livers ( h ), in vivo BLI ( i ), ex vivo BLI of livers ( j ), representative of BLI and metastases ( k ), NETosis in livers ( l ) and body weight changes of mice ( m ) at week 4 after cancer inoculation. n = 3 biological repeats ( c , e – g ), 7 ( h , i , m ), 6 ( l ) or 3 ( j ) mice. P values were obtained by two-tailed unpaired t -test (others). Scale bars, 50 μm. Data are shown as mean ± SD (others).
Techniques Used: Co-Immunoprecipitation Assay, Control, Phospho-proteomics, Knockdown, Over Expression, ChIP-qPCR, Binding Assay, Expressing, Cell Culture, Injection, Flow Cytometry, In Vivo, Ex Vivo, Two Tailed Test
Figure Legend Snippet: a – d In vitro analysis of the DMBT1 neutralizing antibody clone 3H11. Shown are DMBT1–MUC1 interaction in KCs after 3H11 treatment ( a ), CD62L + polarization ( b ), Ccl8 expression of KCs treated with DMBT1-containing tumoral CM with or without treatment of 3H11 ( c ) and NETosis of neutrophils after treatment of CM of the above KCs ( d ). e – i 3H11 treatment (100 μg/mouse) of C57BL/6 mice after intrasplenic inoculation of Py8119 cells. Shown are CD62L + KC abundance ( e ), NETosis ( f , zoomed areas shown at bottom) in livers, in vivo BLI ( g ), ex vivo BLI of livers and representative images of metastases ( h ), and body weight changes of the mice ( i ). j Schematic model of the tumor–KC–neutrophil interaction in breast cancer liver metastasis (figure created with elements from Biorender.com). n = 3 biological repeats ( b – d ) or 6 mice ( e – i ). P values were obtained by repeated measures two-way ANOVA ( g ) or two-tailed unpaired t -test (others). Scale bars, 50 μm. Data are shown as mean ± SEM ( g ) or mean ± SD (others).
Techniques Used: In Vitro, Expressing, In Vivo, Ex Vivo, Two Tailed Test
