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Proteintech dlk1
Dlk1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlk1/PAWR+Antibody/pm41862993-179-13-14
Average 94 stars, based on 4 article reviews

dlk1 - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Spatial Transcriptomic Characteristics of the Aging Human Ovary.
Article Snippet: Sections were permeabilized with 0.1% Triton X- 100 (Sigma, T8787) for 10 min at room temperature, then blocked in 1× PBS containing 5% bovine serum albumin (BSA) for 1 h at room temperature. .. Overnight incubation with primary antibodies was performed at 4°C in a humidified chamber using the following antibodies: STAR (1:100; Santa Cruz, sc- 166821), DLK1 (1:100; Proteintech, 10636- 1- AP), NOTCH3 (1:100; Absin, abs116318), NOTCH2 (1:100; Proteintech, KHC1062), CLDN5 (1:100; Thermofisher, 4C3C2), IgG (1:100; Proteintech, CL488- 10284), and PDGFRA (1:50; nloaded from https://onlinelibrary.w iley.com /doi/10.1111/acel.70288 by N at Prov Indonesia, W iley O nline L ibrary on [29/11/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 16 of 22 Aging Cell, 2025 Santa Cruz, sc- 21789).

Article Title: The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis.
Article Snippet: .. After antigen blocking, the sections were incubated overnight at 4◦C with the following primary antibodies: CCN1 (Cell Signaling Technology, 39382), CCN2 (Abcam, ab6992), ECRG4 (Abcam, ab224077), DBP (Thermo Fisher Scientific, PA540501), DLK1 (Proteintech, 10636-1-AP), EPYC (Abcam, ab122449), HOXC6 (Abcam, ab41587), NCAM1 (Cell Signaling Technology, 3576S), SEMA5A (R&D, AF5896). ..

Article Title: Spatial Transcriptomic Characteristics of the Aging Human Ovary
Article Snippet: Sections were permeabilized with 0.1% Triton X‐100 (Sigma, T8787) for 10 min at room temperature, then blocked in 1× PBS containing 5% bovine serum albumin (BSA) for 1 h at room temperature. .. Overnight incubation with primary antibodies was performed at 4°C in a humidified chamber using the following antibodies: STAR (1:100; Santa Cruz, sc‐166821), DLK1 (1:100; Proteintech, 10636‐1‐AP), NOTCH3 (1:100; Absin, abs116318), NOTCH2 (1:100; Proteintech, KHC1062), CLDN5 (1:100; Thermofisher, 4C3C2), IgG (1:100; Proteintech, CL488‐10284), and PDGFRA (1:50; Santa Cruz, sc‐21789). .. After washing with 1× PBSTx three times for 15 min each, sections were incubated with Alexa Fluor‐conjugated secondary antibodies (Invitrogen, Carlsbad, CA, USA).

Article Title: The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis
Article Snippet: .. After antigen blocking, the sections were incubated overnight at 4°C with the following primary antibodies: CCN1 (Cell Signaling Technology, 39382), CCN2 (Abcam, ab6992), ECRG4 (Abcam, ab224077), DBP (Thermo Fisher Scientific, PA5-40501), DLK1 (Proteintech, 10636-1-AP), EPYC (Abcam, ab122449), HOXC6 (Abcam, ab41587), NCAM1 (Cell Signaling Technology, 3576S), SEMA5A (R&D, AF5896). ..

Article Title: The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis.
Article Snippet: .. In each cycle of procedure, the IVD section underwent antigen retrieval and antigen blocking, and were incubated overnight at 4◦C with the following primary antibodies including CCN1, CCN2, ECRG4, DBP, DLK1, HOXC6, NCAM1, SEMA5A, SOX9 (Cell Signaling Technology, 82630S), TBXT (Abcam, ab209665), COL10A1, COL1A1, TAGLN (Proteintech, 10493-1-AP), and ACTA2 (Cell Signaling Technology, 48938), and then reacted with the secondary antibodies for 1 hour at room temperature, and colored with a fluorescent dye at 3 different wavelengths (λ 520, λ 570, λ 650) for 15 min at room temperature. ..

Blocking Assay:

Article Title: The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis.
Article Snippet: .. In each cycle of procedure, the IVD section underwent antigen retrieval and antigen blocking, and were incubated overnight at 4◦C with the following primary antibodies including CCN1, CCN2, ECRG4, DBP, DLK1, HOXC6, NCAM1, SEMA5A, SOX9 (Cell Signaling Technology, 82630S), TBXT (Abcam, ab209665), COL10A1, COL1A1, TAGLN (Proteintech, 10493-1-AP), and ACTA2 (Cell Signaling Technology, 48938), and then reacted with the secondary antibodies for 1 hour at room temperature, and colored with a fluorescent dye at 3 different wavelengths (λ 520, λ 570, λ 650) for 15 min at room temperature. ..

Image Search Results

A. Schematics of inducing microglia senescence from human iPSCs. B. Quantification of iPSC telomere length measured by qPCR. Data were reported as box & whisker plot showing min to max and analyzed by unpaired t -test. **** p <0.0001. C. Correlation plot showing the genes correlated to expression levels of p16 and p21 in senescent microglia. DLK1 was strongly correlated with both. D. Percentage of microglia expressing DLK1 mRNA in the snRNA dataset. Data are reported as mean ± s.e.m. and analyzed by t-test. ** p =0.0079. E. Schematic of microglia depletion experiment set up. The animals were fed normal food or food containing PLX5622 before CSF extraction. F-G. Quantification of Iba1+ microglia in CA1 (F) and CA3 (G). 4 mice per condition. 2-3 hippocampal sections/mouse were imaged and analyzed. Data are reported as mean ± SEM. **** p <0.0001, *** p <0.001. Data were analyzed by one-way ANOVA. H. Quantification of DLK1 concentration measured in the mouse CSF. G3 Terc -/- mice had elevated DLK1 level in the CSF, and the elevation was diminished by PLX5622 treatment. Data were reported as box & whisker plot showing min to max and were analyzed by one-way ANOVA. (WT vs. G3 Terc -/- * p =0.0328) (G3 Terc -/- vs. G3 Terc -/- +PLX * p =0.0273). Each dot represents an animal. I-J. Quantification of DLK1 concentration measured in the CSF (I) or plasma (J) of young (3-month-old) and old (27-month-old) mice. Each dot represents an animal, and the data were analyzed by t-test. * p =0.0138. Each dot represents an animal, and the data were analyzed by t-test. K. Expression dynamics of DLK1 as a function of age in the human amygdala, cingulate gyrus, parietal lobe, and temporal lobe. p values were obtained by testing the significance of the spline-based smooth term in a generalized additive model.

Journal: bioRxiv

Article Title: Soluble DLK1 secreted by telomere-shortening-induced senescent microglia impairs oligodendrocyte functions and alters neuronal activity

doi: 10.64898/2026.01.14.699608

Figure Lengend Snippet: A. Schematics of inducing microglia senescence from human iPSCs. B. Quantification of iPSC telomere length measured by qPCR. Data were reported as box & whisker plot showing min to max and analyzed by unpaired t -test. **** p <0.0001. C. Correlation plot showing the genes correlated to expression levels of p16 and p21 in senescent microglia. DLK1 was strongly correlated with both. D. Percentage of microglia expressing DLK1 mRNA in the snRNA dataset. Data are reported as mean ± s.e.m. and analyzed by t-test. ** p =0.0079. E. Schematic of microglia depletion experiment set up. The animals were fed normal food or food containing PLX5622 before CSF extraction. F-G. Quantification of Iba1+ microglia in CA1 (F) and CA3 (G). 4 mice per condition. 2-3 hippocampal sections/mouse were imaged and analyzed. Data are reported as mean ± SEM. **** p <0.0001, *** p <0.001. Data were analyzed by one-way ANOVA. H. Quantification of DLK1 concentration measured in the mouse CSF. G3 Terc -/- mice had elevated DLK1 level in the CSF, and the elevation was diminished by PLX5622 treatment. Data were reported as box & whisker plot showing min to max and were analyzed by one-way ANOVA. (WT vs. G3 Terc -/- * p =0.0328) (G3 Terc -/- vs. G3 Terc -/- +PLX * p =0.0273). Each dot represents an animal. I-J. Quantification of DLK1 concentration measured in the CSF (I) or plasma (J) of young (3-month-old) and old (27-month-old) mice. Each dot represents an animal, and the data were analyzed by t-test. * p =0.0138. Each dot represents an animal, and the data were analyzed by t-test. K. Expression dynamics of DLK1 as a function of age in the human amygdala, cingulate gyrus, parietal lobe, and temporal lobe. p values were obtained by testing the significance of the spline-based smooth term in a generalized additive model.

Article Snippet: DLK1 treatment started on Day 14 (200ng/ml, R&D System, 1144-PR), and the cells were imaged and sequenced on Day 21.

Techniques: Whisker Assay, Expressing, Extraction, Concentration Assay, Clinical Proteomics

A. Gene dendrogram showing all the gene modules identified in the iPSC-derived microglia transcriptome data. The merged dynamic was generated using a height threshold of 0.5. B. Heatmap showing the WGCNA modules significantly (p <= 0.05) correlated with p21, p16, or DLK1. C. Scatterplot of gene significance for DLK1 expression level versus module membership in the brown module (top) and the green module (bottom). D–E. Top six KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways predicted from the top 500 genes (ranked by module membership) in the brown (D) and green module (E).

Journal: bioRxiv

Article Title: Soluble DLK1 secreted by telomere-shortening-induced senescent microglia impairs oligodendrocyte functions and alters neuronal activity

doi: 10.64898/2026.01.14.699608

Figure Lengend Snippet: A. Gene dendrogram showing all the gene modules identified in the iPSC-derived microglia transcriptome data. The merged dynamic was generated using a height threshold of 0.5. B. Heatmap showing the WGCNA modules significantly (p <= 0.05) correlated with p21, p16, or DLK1. C. Scatterplot of gene significance for DLK1 expression level versus module membership in the brown module (top) and the green module (bottom). D–E. Top six KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways predicted from the top 500 genes (ranked by module membership) in the brown (D) and green module (E).

Article Snippet: DLK1 treatment started on Day 14 (200ng/ml, R&D System, 1144-PR), and the cells were imaged and sequenced on Day 21.

Techniques: Derivative Assay, Generated, Expressing

A. Schematic showing the IV injection of the AAV-EGFP or AAV-sDLK1-T2A-GFP into the mice. B. Quantification of the protein levels of sDLK1 in the cortex of the mice, measured by mouse DLK1 ELISA. C. Distributions of the normalized numbers of genes up-regulated (top) and down-regulated (bottom) in different cell types of the mice injected with AAV-sDLK1-T2A-GFP, compared to mice injected with AAV-EGFP. The gene burden score is defined as the number of differentially expressed genes per 1000 UMI detected in each cell type. D. Scatter plot showing the positively correlated genes between DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected oligodendrocytes. E. Western blot of MBP (top) and MOBP (bottom). F–G. Quantification of the MBP (F) and MOBP (G) western blot. Data were analyzed by two-tailed unpaired t -test. ** p =0.0095 (MBP); *** p =0.0009 (MOBP). H. Ridge plot of the predicted chronological ages for oligodendrocytes in the mice injected with AAV-EGFP (top) and AAV-sDLK1-T2A-GFP (bottom). I. Quantification of the predicted age of oligodendrocytes. Data were reported as a box & whisker plot showing min to max and analyzed by t-test. Each dot represents a cell. **** p < 0.0001. J. UMAP plot showing the enrichment of OPC2 caused by increased sDLK1. K. Ratios of each OPC cluster. Data are reported as mean ± s.e.m. and analyzed by two-way ANOVA. * p =0.0432. L. Running enrichment score and pre-ranked list showing a negative enrichment of oligodendrocyte differentiation predicted by OPC3 markers

Journal: bioRxiv

Article Title: Soluble DLK1 secreted by telomere-shortening-induced senescent microglia impairs oligodendrocyte functions and alters neuronal activity

doi: 10.64898/2026.01.14.699608

Figure Lengend Snippet: A. Schematic showing the IV injection of the AAV-EGFP or AAV-sDLK1-T2A-GFP into the mice. B. Quantification of the protein levels of sDLK1 in the cortex of the mice, measured by mouse DLK1 ELISA. C. Distributions of the normalized numbers of genes up-regulated (top) and down-regulated (bottom) in different cell types of the mice injected with AAV-sDLK1-T2A-GFP, compared to mice injected with AAV-EGFP. The gene burden score is defined as the number of differentially expressed genes per 1000 UMI detected in each cell type. D. Scatter plot showing the positively correlated genes between DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected oligodendrocytes. E. Western blot of MBP (top) and MOBP (bottom). F–G. Quantification of the MBP (F) and MOBP (G) western blot. Data were analyzed by two-tailed unpaired t -test. ** p =0.0095 (MBP); *** p =0.0009 (MOBP). H. Ridge plot of the predicted chronological ages for oligodendrocytes in the mice injected with AAV-EGFP (top) and AAV-sDLK1-T2A-GFP (bottom). I. Quantification of the predicted age of oligodendrocytes. Data were reported as a box & whisker plot showing min to max and analyzed by t-test. Each dot represents a cell. **** p < 0.0001. J. UMAP plot showing the enrichment of OPC2 caused by increased sDLK1. K. Ratios of each OPC cluster. Data are reported as mean ± s.e.m. and analyzed by two-way ANOVA. * p =0.0432. L. Running enrichment score and pre-ranked list showing a negative enrichment of oligodendrocyte differentiation predicted by OPC3 markers

Article Snippet: DLK1 treatment started on Day 14 (200ng/ml, R&D System, 1144-PR), and the cells were imaged and sequenced on Day 21.

Techniques: IV Injection, Enzyme-linked Immunosorbent Assay, Injection, Western Blot, Two Tailed Test, Whisker Assay

A. Dot plot showing the expression level of DLK1 in different cell types in the hippocampus tissue. The size of each dot represents the percentage of cells with detected DLK1 mRNA. B. Chord diagram showing DLK signaling predicted by CellChat. The lengths of the segmented outer circle reflect the expression levels of ligand proteins in each cell type and of receptor proteins in the receiving cells, showing strong expression of DLK1 signaling originating from interneurons and astrocytes to oligodendrocytes and OPCs. C. Bubble plot showing the DLK1 interactions originating from interneurons and astrocytes to different receptors. D. Venn diagram showing the overlap of upregulated DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected oligodendrocyte(top) and the overlap of downregulated DEGs in G3 Terc-/-and AAV-sDLK1-T2A-GFP injected oligodendrocyte (bottom). E. Dot plot showing the change of expression levels of myelination proteins in oligodendrocytes caused by the increase of sDLK1. F. Cnet plot showing the network of genes associated with myelination-related Gene Ontology terms and myelin-related diseases, based on enrichment analysis of the top 500 differentially expressed genes in oligodendrocytes from mice with AAV-sDLK1-T2A-GFP versus mice with AAV-GFP. Nodes represent genes or GO terms; edge colors represent the pathways each node is involved in.

Journal: bioRxiv

Article Title: Soluble DLK1 secreted by telomere-shortening-induced senescent microglia impairs oligodendrocyte functions and alters neuronal activity

doi: 10.64898/2026.01.14.699608

Figure Lengend Snippet: A. Dot plot showing the expression level of DLK1 in different cell types in the hippocampus tissue. The size of each dot represents the percentage of cells with detected DLK1 mRNA. B. Chord diagram showing DLK signaling predicted by CellChat. The lengths of the segmented outer circle reflect the expression levels of ligand proteins in each cell type and of receptor proteins in the receiving cells, showing strong expression of DLK1 signaling originating from interneurons and astrocytes to oligodendrocytes and OPCs. C. Bubble plot showing the DLK1 interactions originating from interneurons and astrocytes to different receptors. D. Venn diagram showing the overlap of upregulated DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected oligodendrocyte(top) and the overlap of downregulated DEGs in G3 Terc-/-and AAV-sDLK1-T2A-GFP injected oligodendrocyte (bottom). E. Dot plot showing the change of expression levels of myelination proteins in oligodendrocytes caused by the increase of sDLK1. F. Cnet plot showing the network of genes associated with myelination-related Gene Ontology terms and myelin-related diseases, based on enrichment analysis of the top 500 differentially expressed genes in oligodendrocytes from mice with AAV-sDLK1-T2A-GFP versus mice with AAV-GFP. Nodes represent genes or GO terms; edge colors represent the pathways each node is involved in.

Article Snippet: DLK1 treatment started on Day 14 (200ng/ml, R&D System, 1144-PR), and the cells were imaged and sequenced on Day 21.

Techniques: Expressing, Injection

A Venn diagram showing the overlap of upregulated (top) and downregulated (bottom) DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP -injected excitatory neurons. B. Dot plot of the top 10 Reactome pathways inferred by the upregulated overlapping DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected excitatory neurons. C–D. Running enrichment score and pre-ranked list showing a positive (C) and negative (D) enrichment of calcium ion transmembrane transport predicted by the upregulated overlapping DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected excitatory neurons. E. Schematic illustrating the experiment setup to test the chronic effects of DLK1 on neuronal activities. F. Representative fluorescence image of human iPSC-derived neurons expressing GCaMP8f showing spontaneous activity. G. Representative spontaneous calcium traces. H–J. Quantification of synchronized firing rate (H). firing amplitude (I), or spontaneous firing rate (J). K. Representative averaged calcium traces from one KCl stimulation experiment in neurons treated with DLK1 (red) and the untreated control neurons (black). Recording 400 seconds. Mean± s.e.m. L–N. Quantification of peak amplitude (L), the delayed KCl stimulation-induced neuronal responses (M), the time each neuron spent to reach peak intensity (N), from KCl stimulation-induced neuronal responses. Data are presented as mean ± s.e.m. and analyzed by unpaired t -test. ** p =0.0055 (L) **** p <0.0001 (N).

Journal: bioRxiv

Article Title: Soluble DLK1 secreted by telomere-shortening-induced senescent microglia impairs oligodendrocyte functions and alters neuronal activity

doi: 10.64898/2026.01.14.699608

Figure Lengend Snippet: A Venn diagram showing the overlap of upregulated (top) and downregulated (bottom) DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP -injected excitatory neurons. B. Dot plot of the top 10 Reactome pathways inferred by the upregulated overlapping DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected excitatory neurons. C–D. Running enrichment score and pre-ranked list showing a positive (C) and negative (D) enrichment of calcium ion transmembrane transport predicted by the upregulated overlapping DEGs in G3 Terc-/- and AAV-sDLK1-T2A-GFP injected excitatory neurons. E. Schematic illustrating the experiment setup to test the chronic effects of DLK1 on neuronal activities. F. Representative fluorescence image of human iPSC-derived neurons expressing GCaMP8f showing spontaneous activity. G. Representative spontaneous calcium traces. H–J. Quantification of synchronized firing rate (H). firing amplitude (I), or spontaneous firing rate (J). K. Representative averaged calcium traces from one KCl stimulation experiment in neurons treated with DLK1 (red) and the untreated control neurons (black). Recording 400 seconds. Mean± s.e.m. L–N. Quantification of peak amplitude (L), the delayed KCl stimulation-induced neuronal responses (M), the time each neuron spent to reach peak intensity (N), from KCl stimulation-induced neuronal responses. Data are presented as mean ± s.e.m. and analyzed by unpaired t -test. ** p =0.0055 (L) **** p <0.0001 (N).

Article Snippet: DLK1 treatment started on Day 14 (200ng/ml, R&D System, 1144-PR), and the cells were imaged and sequenced on Day 21.

Techniques: Injection, Fluorescence, Derivative Assay, Expressing, Activity Assay, Control

A. Volcano plot of significant DEGs in iPSC-derived neurons treated with soluble DLK1. Red and grey dots represent genes with a log2FC > 0.1 or < -0.1, respectively. Top 10 differential genes are labeled. B. Dot plot of the top 10 GO Cellular Component ontologies inferred by the upregulated overlapping DEGs in sDLK1-treated neurons. C. Heatmap showing the fold-change of the genes upregulated (top) and downregulated (bottom) in the brains from patients with Alzheimer’s disease. All gene expressions are significantly changed (p-value <= 0.05) in the sDLK1-treated neurons.

Journal: bioRxiv

Article Title: Soluble DLK1 secreted by telomere-shortening-induced senescent microglia impairs oligodendrocyte functions and alters neuronal activity

doi: 10.64898/2026.01.14.699608

Figure Lengend Snippet: A. Volcano plot of significant DEGs in iPSC-derived neurons treated with soluble DLK1. Red and grey dots represent genes with a log2FC > 0.1 or < -0.1, respectively. Top 10 differential genes are labeled. B. Dot plot of the top 10 GO Cellular Component ontologies inferred by the upregulated overlapping DEGs in sDLK1-treated neurons. C. Heatmap showing the fold-change of the genes upregulated (top) and downregulated (bottom) in the brains from patients with Alzheimer’s disease. All gene expressions are significantly changed (p-value <= 0.05) in the sDLK1-treated neurons.

Article Snippet: DLK1 treatment started on Day 14 (200ng/ml, R&D System, 1144-PR), and the cells were imaged and sequenced on Day 21.

Techniques: Derivative Assay, Labeling

Notch hyperactivation with reduced Dlk1 in Pg-derived cells CReP brains skews fate toward gliogenesis. ( a ) Schematic of the Notch signaling pathway in neural stem cell fate determination. ( b ) Expression level of Notch1, Dll1, Dlk1 and Hes1 in GFP-positive sorted cells from CReF and CReP brains, as analyzed by RNA-sequencing. WILD-TYPE refers to fertilized embryo-derived cells from CReF embryos, and Pg refers to Pg embryo-derived cells in CReP embryos. Each dot represents an individual replicate. * P <0.05. ( c ) Representative immunofluorescence images of Notch intracellular domain (NICD) of brain cells cultured for five days. Scale bar, 100 μm. ( d ) Quantification of NICD-positive nuclei as a proportion of DAPI-positive cells in CReP and CReF cultures. Each dot represents an individual replicate. * P <0.05. ( e ) Representative immunofluorescence images of Tubulin beta III (TUBB3)-positive neurons in the brain cells cultured for five days. Scale bar, 100 μm. ( f ) Quantification of TUBB3-positive neurons as a proportion of DAPI-positive cells of CReP and CReF. Each dot represents an individual replicate. * P <0.05. ( g ) Representative immunofluorescence images of PDGFRα-positive oligodendrocyte precursor cells (OPCs) in the brain cells cultured for five days. Scale bar, 100 μm. ( h ) Quantification of PDGFRα-positive OPCs as a proportion of DAPI-positive cells of CReP and CReF. Each dot represents an individual replicate. * P <0.05. ( i ) Representative immunofluorescence images of TUBB3 in cultured CReP brain cells after 72 h of recombinant Dlk1 (rDlk1, 200 ng/mL) or PBS control treatment. Scale bar, 100 μm. ( j ) Quantification of TUBB3-positive neurons as a proportion of DAPI-positive cells in each condition. Each dot represents an individual replicate. * P <0.05. ( k ) Representative immunofluorescence images of PDGFRα in cultured CReP brain cells after 72 h of rDlk1 (200 ng/mL) or PBS control treatment. Scale bar, 100 μm. ( l ) Quantification of PDGFRα-positive OPCs as a proportion of DAPI-positive cells in each condition. Each dot represents an individual replicate. * P <0.05.

Journal: bioRxiv

Article Title: Parthenogenote-Derived Brain Unveils the Critical Role of Paternal Genome in Neural Development

doi: 10.64898/2026.01.06.697935

Figure Lengend Snippet: Notch hyperactivation with reduced Dlk1 in Pg-derived cells CReP brains skews fate toward gliogenesis. ( a ) Schematic of the Notch signaling pathway in neural stem cell fate determination. ( b ) Expression level of Notch1, Dll1, Dlk1 and Hes1 in GFP-positive sorted cells from CReF and CReP brains, as analyzed by RNA-sequencing. WILD-TYPE refers to fertilized embryo-derived cells from CReF embryos, and Pg refers to Pg embryo-derived cells in CReP embryos. Each dot represents an individual replicate. * P <0.05. ( c ) Representative immunofluorescence images of Notch intracellular domain (NICD) of brain cells cultured for five days. Scale bar, 100 μm. ( d ) Quantification of NICD-positive nuclei as a proportion of DAPI-positive cells in CReP and CReF cultures. Each dot represents an individual replicate. * P <0.05. ( e ) Representative immunofluorescence images of Tubulin beta III (TUBB3)-positive neurons in the brain cells cultured for five days. Scale bar, 100 μm. ( f ) Quantification of TUBB3-positive neurons as a proportion of DAPI-positive cells of CReP and CReF. Each dot represents an individual replicate. * P <0.05. ( g ) Representative immunofluorescence images of PDGFRα-positive oligodendrocyte precursor cells (OPCs) in the brain cells cultured for five days. Scale bar, 100 μm. ( h ) Quantification of PDGFRα-positive OPCs as a proportion of DAPI-positive cells of CReP and CReF. Each dot represents an individual replicate. * P <0.05. ( i ) Representative immunofluorescence images of TUBB3 in cultured CReP brain cells after 72 h of recombinant Dlk1 (rDlk1, 200 ng/mL) or PBS control treatment. Scale bar, 100 μm. ( j ) Quantification of TUBB3-positive neurons as a proportion of DAPI-positive cells in each condition. Each dot represents an individual replicate. * P <0.05. ( k ) Representative immunofluorescence images of PDGFRα in cultured CReP brain cells after 72 h of rDlk1 (200 ng/mL) or PBS control treatment. Scale bar, 100 μm. ( l ) Quantification of PDGFRα-positive OPCs as a proportion of DAPI-positive cells in each condition. Each dot represents an individual replicate. * P <0.05.

Article Snippet: For Dlk1 supplementation, brain cells were treated for 72 h with recombinant Dlk1 (R&D systems, 1144-PR-025/CF, 200ng/ml) or PBS, then fixed in 4% PFA for 10 min.

Techniques: Derivative Assay, Expressing, RNA Sequencing, Immunofluorescence, Cell Culture, Recombinant, Control

Schematic model: Dlk1 dosage constrains Notch signaling to maintain neural cell fate balance in the developing mouse brain.

Journal: bioRxiv

Article Title: Parthenogenote-Derived Brain Unveils the Critical Role of Paternal Genome in Neural Development

doi: 10.64898/2026.01.06.697935

Figure Lengend Snippet: Schematic model: Dlk1 dosage constrains Notch signaling to maintain neural cell fate balance in the developing mouse brain.

Article Snippet: For Dlk1 supplementation, brain cells were treated for 72 h with recombinant Dlk1 (R&D systems, 1144-PR-025/CF, 200ng/ml) or PBS, then fixed in 4% PFA for 10 min.

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