Journal: Cardiovascular Research
Article Title: Contractility measurements for cardiotoxicity screening with ventricular myocardial slices of pigs
doi: 10.1093/cvr/cvad141
Figure Lengend Snippet: Screening test with drugs from CiPA list with known interactions and risks performed in three different laboratories ( N = 3–5, n = 6–10). The effects of bepridil, cisapride, disopyramide, ibutilide, and risperidone (red) as well as negative controls DMSO and acetylsalicylic acid (black) were compared to time-matched controls treated only with PBS (grey). ( A ) Kaplan–Meier curve displaying the pacing capability of slices. Statistical analysis by Fisher’s exact test. ( B–H ) Mean and SEM of effects compared to PBS with a two-way ANOVA with Dunnett’s multiple comparison test on the normalized pacing threshold ( B ), normalized force ( C ), FFR ( D ), normalized contraction duration ( E ), CDFR ( F ), normalized ERP ( G ), and changes in diastolic force ( H ). Absolute values of each slice were normalized to values before adding the drug or solvent. Exact n and P values for each condition are given in , .
Article Snippet: Drugs from the CiPA list were added at five different concentration steps into 3–4 mL from the following stocks: bepridil hydrochloride (Tocris Bioscience, UK) 10 mM dissolved in DMSO; ibutilide hemifumarate (Sigma-Aldrich, USA) 10 μM dissolved in H 2 O; disopyramide phosphate (Sigma-Aldrich, USA) 10 mM dissolved in H 2 O; cisapride monohydrate 100 μM dissolved in DMSO; risperidone (Sigma-Aldrich, USA) 1 mM dissolved in DMSO; and acetylsalicylic acid (Sigma-Aldrich, USA) 100 μM dissolved in H 2 O.
Techniques: Comparison, Solvent