Journal: Journal of Cancer Research and Clinical Oncology
Article Title: DHRS9 promotes malignant progression of ovarian cancer through SQSTM1
doi: 10.1007/s00432-025-06290-y
Figure Lengend Snippet: Effects of DHRS9 knockdown on aggressive phenotypes and apoptosis of A2780 cells. a , b qRT-PCR and western blot evaluating mRNA and protein expression of DHRS9 in IOSE-80, A2780 and OVCAR-3 cell lines. c , d qRT-PCR and western blot verification of DHRS9 knockdown efficiency. e , f CCK-8 and colony formation assays examining the effects of DHRS9 depletion on A2780 cell growth. g , h Transwell assays assessing the impact of DHRS9 knockdown on A2780 cell migration and invasion capabilities. i Flow cytometry-based apoptosis detection in DHRS9-silencing A2780 cells.Data are shown as mean ± SD. Scale bar = 200 μm. ** P < 0.01; *** P < 0.001;**** P < 0.0001
Article Snippet: siRNAs targeting DHRS9 and SQSTM1 were synthesized by JTSBIO Co., Ltd. (China) and Sangon Biotech (China), respectively.
Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay, Migration, Flow Cytometry