Journal: Poultry Science
Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers
doi: 10.1016/j.psj.2026.106976
Figure Lengend Snippet: Effect of Lut on 42 °C -induced ROS accumulation in cells. (A) Effect of Lut (5, 10, 20, 40, and 100 μM) on cell viability, n = 6. (B) Protective effect of Lut (5, 10, 20, 40, and 100 μM) on 42 °C-induced cytotoxicity, n = 6. (C, D) Effect of Lut on 42 °C-induced ROS generation measured by 10 μM DCFH-DA fluorescent probe. The results were expressed as the mean DCF fluorescence intensity (n = 6). (E, F) Effect of Lut on 42 °C-induced superoxide anion radical generation measured by 10 μM DHE fluorescent probe. The results were expressed as the mean DHE fluorescence intensity (n = 6). The normal control group was incubated at 37 °C for 6 h. The HS group was incubated at 37 °C for 5 h and then at 42 °C for 1 h. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), ** represents the significant difference compared with the control group ( P < 0.01). # represents the significant difference compared with the HS ( P < 0.05). ## represents the significant difference compared with the HS ( P < 0.01).
Article Snippet: 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), dihydroethidium (DHE), cell counting kit-8 (CCK-8), RIPA lysis buffer, phenylmethanesulfonyl fluoride (PMSF), and BCA protein assay kit were purchased from Beyotime Biotech.
Techniques: Fluorescence, Control, Incubation