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dgcr8  (Proteintech)


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    Proteintech dgcr8
    Dgcr8, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dgcr8/DGCR8+C-terminal+Antibody/pm41773772-57-31-34
    Average 93 stars, based on 55 article reviews
    dgcr8 - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Neddylation-Mediated hnRNPA2B1 Degradation Aggravates Retinal Endothelial Cell Dysfunction in Diabetic Retinopathy by Regulating miR-93-5p/VEGFA.
    Article Snippet: After BCA quantification, 10 μg/μL samples were separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked in 5% nonfat milk for three hours, then incubated overnight at 4°C with primary antibodies: hnRNPA2B1 (83773-7-RR; Proteintech, Wuhan, China), NEDD8 (ab81264; Abcam, Cambridgeshire, UK), VEGFA (81323-2-RR; Proteintech), DGCR8 (10996-1- AP; Proteintech), and tubulin (11224-1-AP; Proteintech). .. After secondary antibody incubation (37°C, one hour) and Tris-buffered saline with Tween-20 washes, detection was performed using BeyoECL Star (Beyotime Biotechnology) with an Amersham Imager 600 RGB (Amersham, Piscataway, NJ, USA).

    Article Title: Neddylation-Mediated hnRNPA2B1 Degradation Aggravates Retinal Endothelial Cell Dysfunction in Diabetic Retinopathy by Regulating miR-93-5p/VEGFA
    Article Snippet: After BCA quantification, 10 μg/μL samples were separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked in 5% nonfat milk for three hours, then incubated overnight at 4°C with primary antibodies: hnRNPA2B1 (83773-7-RR; Proteintech, Wuhan, China), NEDD8 (ab81264; Abcam, Cambridgeshire, UK), VEGFA (81323-2-RR; Proteintech), DGCR8 (10996-1-AP; Proteintech), and tubulin (11224-1-AP; Proteintech). .. After secondary antibody incubation (37°C, one hour) and Tris-buffered saline with Tween-20 washes, detection was performed using BeyoECL Star (Beyotime Biotechnology) with an Amersham Imager 600 RGB (Amersham, Piscataway, NJ, USA).



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    (A–D) Scatter plot showing the correlation between <t>DGCR8</t> gene expression levels and NK cell, as percentage (A, patients; C, controls), and as cell count (B, patients; D, controls). The red dots indicate the patient without DGCR8 deletion. (E) Correlation between the activation of PI3K pathway assessed by measure of S6 phosphorylation, after PBMCs stimulation and absolute counts of B cells. S6 phosphorylation was not performed in patient without DGCR8 deletion. Abbreviations: pS6 (phosphorylated S6 protein); PMA-iono (phorbol-12-myristate-13-acetate and ionomycin).
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    (A–D) Scatter plot showing the correlation between DGCR8 gene expression levels and NK cell, as percentage (A, patients; C, controls), and as cell count (B, patients; D, controls). The red dots indicate the patient without DGCR8 deletion. (E) Correlation between the activation of PI3K pathway assessed by measure of S6 phosphorylation, after PBMCs stimulation and absolute counts of B cells. S6 phosphorylation was not performed in patient without DGCR8 deletion. Abbreviations: pS6 (phosphorylated S6 protein); PMA-iono (phorbol-12-myristate-13-acetate and ionomycin).

    Journal: Frontiers in Immunology

    Article Title: From DGCR8 expression analysis to diseased pathways in 22q11.2 deletion syndrome

    doi: 10.3389/fimmu.2025.1611527

    Figure Lengend Snippet: (A–D) Scatter plot showing the correlation between DGCR8 gene expression levels and NK cell, as percentage (A, patients; C, controls), and as cell count (B, patients; D, controls). The red dots indicate the patient without DGCR8 deletion. (E) Correlation between the activation of PI3K pathway assessed by measure of S6 phosphorylation, after PBMCs stimulation and absolute counts of B cells. S6 phosphorylation was not performed in patient without DGCR8 deletion. Abbreviations: pS6 (phosphorylated S6 protein); PMA-iono (phorbol-12-myristate-13-acetate and ionomycin).

    Article Snippet: As regards quantitative Real-time PCR, 25 ng of cDNA was added to 5 μl of 2X TaqManTM Fast Advanced Master Mix (Thermo Fisher Scientific, Waltham, Massachusetts, USA; Cat. N°4444557), 0.5 μl of 20X probe ( DGCR8 Hs00987085_m1 or G6PD Hs00959073_g1 or HPRT1 Hs02800695_m1; TaqMan ® Gene Expression Assay, Thermo Fisher Scientific, Waltham, Massachusetts, USA) and made up to a final volume of 10 μl with sterile water.

    Techniques: Gene Expression, Cell Counting, Activation Assay, Phospho-proteomics