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desmin  (Bioss)


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    Structured Review

    Bioss desmin
    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Desmin, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/desmin/product/Bioss
    Average 94 stars, based on 8 article reviews
    desmin - by Bioz Stars, 2026-05
    94/100 stars

    Images

    1) Product Images from "γ -Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells"

    Article Title: γ -Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2026.1770540

    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
    Figure Legend Snippet: Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).

    Techniques Used: Activity Assay, Immunofluorescence, Staining, CCK-8 Assay, EdU Assay, Quantitative RT-PCR, Expressing, Western Blot, Control



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    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification <t>(Desmin,</t> <t>Pax7,</t> and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).
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    Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in <t>desmin‐positive</t> smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle <t>Actin</t> <t>(SMA;</t> red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).
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    Image Search Results


    Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).

    Journal: Frontiers in Veterinary Science

    Article Title: γ -Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells

    doi: 10.3389/fvets.2026.1770540

    Figure Lengend Snippet: Effects of GABA on the proliferative activity and molecular markers in BSCs. (A) Immunofluorescence staining for BSCs identification (Desmin, Pax7, and MyoG (green) and nuclei (DAPI, blue) (10×; scale bar = 200 μm). (B–E) CCK-8 analysis of viability after 24, 48, 72, and 96 h treatment with 0.1, 0.5, 1, 2, 5, and 10 mM GABA and CON = 0 mM. (F,G) EdU assay. Hoechst (nuclei, blue) and EdU-positive cells (red) scale bar = 200 μm. (H–M) RT-qPCR. mRNA expression of proliferation-related genes ( CDK2 , PCNA , Ki-67 , Pax7 , Pax3 , CCND1 ) after 48 h treatment with 0.5 and 1 mM GABA. (N,O) Western blotting of PAX7, CDK1, CDK2, and P21 proteins (β-actin loading control). (Mean ± SD; n = 3; groups with different letters a–e differs at p < 0.05).

    Article Snippet: Polyclonal antibodies against myogenin (MYOG; BS-3550R, Bioss, Beijing, China), paired box protein 7 (Pax7; AB-528428, Abcam, United Kingdom) and desmin (BS-20702R, Bioss, Beijing, China) were each diluted 1:100 in PBS containing 5% bovine serum albumin (BSA) and incubated overnight at room temperature.

    Techniques: Activity Assay, Immunofluorescence, Staining, CCK-8 Assay, EdU Assay, Quantitative RT-PCR, Expressing, Western Blot, Control

    Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in desmin‐positive smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle Actin (SMA; red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).

    Journal: Cancer Medicine

    Article Title: Transgelin Expression in Activated Cancer‐Associated Fibroblasts Regulates Stromal Contractility and Promotes Colon Cancer Progression

    doi: 10.1002/cam4.71789

    Figure Lengend Snippet: Transgelin expression pattern in colorectal cancer. (A) Transgelin expression in normal colonic tissues. (B) Transgelin expression in colorectal cancer tissues. (C) Transgelin expression in cancer tissues. (D) Fluorescent immunostaining showed that transgelin was expressed in desmin‐positive smooth muscles as well as in other cancer stromal regions; transgelin, desmin are shown in dark green, green, and purple, respectively. (E) Immunostaining results for transgelin (dark green), smooth muscle Actin (SMA; red), and 4′,6‐diamidino‐2‐phenylindole (DAPI; purple) in the cancer cell stroma. (F) Immunostaining for AE1/3 (green), transgelin (dark green), and DAPI (purple).

    Article Snippet: The primary antibodies used were mouse monoclonal anti‐human transgelin antibody (OriGene Technologies, Rockville, MD, USA; used at 1:200 dilution), rabbit monoclonal anti–human α‐SMA (SPRING BIOSCIENCE, Pleasanton, CA, USA; used at 1:200 dilution), Desmin (Nichirei, Tokyo, Japan; used without dilution), 4′,6‐diamidino‐2‐phenylindole (DAPI; Akoya Biosciences, Menlo Park, CA, USA; used without dilution), and AE1/3 antibody (Dako, Agilent Technologies, Santa Clara, CA, USA; used without dilution).

    Techniques: Expressing, Immunostaining, Muscles