Journal: Journal of Cerebral Blood Flow & Metabolism
Article Title: Neural stem cell transplantation attenuates cognitive decline and neuroinflammation in a mouse model of Alzheimer’s disease with chronic cerebral hypoperfusion
doi: 10.1177/0271678X251400239
Figure Lengend Snippet: Immunofluorescence analysis of SVZ niche alterations following stem cell transplantation. (a–h) Immunofluorescence staining of SVZ for Ki67, DCX, CD31, GFAP, and Iba1 at 9 and 12 months. (i–m) Quantitative analysis of fluorescence intensity of neurogenic, vasculogenic, and glial markers in the SVZ, n = 5, a p < 0.05 vs WT, aa p < 0.01 vs WT. Scale bar: 50 µm. M: month; WT: wild-type; AD: Alzheimer’s disease; CCH: chronic cerebral hypoperfusion; NSCs: neural stem cells; iPSCs: induced pluripotent stem cells; SVZ: subventricular zone.
Article Snippet: The antibodies applied in this study included: TNF-α (1:1000, 346654; Zen-Bioscience), IL-1β (1:1000, bs-0812R; BOISS), NLRP3 (1:1000, bs-10021R; BOISS), APP (1:1000, ab232136; Abcam), IL-6 (1:1000, 500286; Zen-Bioscience), CD16/32 (1:1000, ab228971; Abcam), β-tubulin (1:10,000, 66240-1-Ig; Proteintech), β-actin (1:10,000, 20536-1-AP; Proteintech), CD31 (1:1000, 347526; Zen-Bioscience), DCX (1:1000, 13925-1-AP; Proteintech), GFAP (1:1000, A19058; ABclonal), Iba1 (1:200, 019-19741; Wako), LC3 (1:1000, 14600-1-AP; Proteintech), cleaved Caspase-3 (1:1000, 9669S; CST), IL-10 (1:1000, bs-0698R; BOISS), IL-4 (1:1000, 66142-1-Ig; Proteintech), CD163 (1:1000, ab182422; Abcam), and BDNF (1:1000, 28205-1-AP; Proteintech), VEGF (1:1000, R389402; Zen-Bioscience), VAChT (1:1000, 190002; Zen-Bioscience), and PSD95 (1:1000, R381001; Zen-Bioscience).
Techniques: Immunofluorescence, Transplantation Assay, Staining, Fluorescence