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Thermo Fisher dapt
Dapt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapt/DAPT/10__1183_slash_23120541__01708___2025-339-18-21
Average 95 stars, based on 1 article reviews

dapt - by Bioz Stars, 2026-10

95/100 stars

Images

Extraction:

Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
Article Snippet: .. After confirming the involvement of MT1MMP, the above cultures were also treated with or without Marimastat (10 μM) (MMP inhibitor) or DAPT (10 μM) (γ-secretase inhibitor) or both for 0 and 5 min for membranous, cytoplasmic and nuclear preparation using NEPER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific, Waltham, MA) before Western blot of nuclear pSMAD2/3, CD44ICD, active MT1MMP and active γ-secretase, and for 0, 5, 15, 30 and 45 min for immunostaining of CD44ICD, cyclin D1 and p75NTR. .. Total RNAs were extracted using RNeasy Mini Kit and reverse- transcribed using High-Capacity Reverse Transcription Kit. cDNA was amplified by real-time RT-PCR using specific primers and PCR Master Mix in ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Quant Studio 5 Real-time PCR System (ThermoFisher Scientific, Waltham, MA).

Western Blot:

Immunostaining:

Concentration Assay:

Article Title: Macrophage efferocytosis is controlled by epigenetic modifications mediated by RBPJ
Article Snippet: 0.5 mg/mL oxLDL (ThermoFisher Scientific, L34357) or nLDL (MyBioSource, MBS143113) for 24 hours at 37°C. ( ) 2. .. DAPT (Thermo Scientific, J65864.MA), a γ-secretase inhibitor, at a concentration of 10 μM for 24 hours under cell culture conditions( ) 3. .. N2-(4-isopropylphenyl)-5-(3-methoxyphenoxy)quinazoline-2,4-diamine (Yhhu-3792) (MCE, HY-120782), a Notch signaling activator, at a concentration of 2.5 μM for 24 hours under cell culture conditions of ( ) 4.

Article Title: Differentiation method
Article Snippet: .. Improved differentiation medium (DM+) Concentration Component Gibco Advanced DMEM/F12 10 mM HEPES 1 x B27 wo VitA 1 x N2 1.25 mM n-Acetylcysteine 50 ng/ml hEGF 10 nM Gastrin 25 ng/ml HGF 0.5 uM A83.01 25 ng/ml BMP7 100 ng/ml FGF19 10 uM DAPT 3 uM Dexamethasone 3 uM IWP-2 3 uM Chir 50 uM iCRT3 100 uM Carbachol Old differentiation medium (DM) Concentration Component Gibco Advanced DMEM/F12 10 mM HEPES 1 x B27 wo VitA 1 x N2 1.25 mM n-Acetylcysteine 50 ng/ml hEGF 10 nM Gastrin 25 ng/ml HGF 0.5 uM A83.01 25 ng/ml BMP7 100 ng/ml FGF19 10 uM DAPT 3 uM Dexamethasone Expansion medium (EM) Concentration Component Gibco Advanced DMEM/F12 10 mM HEPES 1 x B27 wo VitA 1 x N2 1.25 mM n-Acetylcysteine 50 ng/ml hEGF 10 nM Gastrin 25 ng/ml HGF 10 % Rspondin conditioned medium 0.01 M Nicotinamide 100 ng/ml FGF10 5 uM A83.01 10 uM FSK ..

Cell Culture:

Article Title: Release, Transfer, Fold: Using a Silicone Adhesive for On-Demand 3D Tissue Engineering.
Article Snippet: Expanded hSAECs were seeded at a density of 250,000 cells/cm2 and cultured in BEpiCM medium (ScienCell Research Laboratories). .. Once confluent, the medium was switched to PneumaCult-ALI medium (STEMCELL Technologies) supplemented with 10 μM DAPT (Thermo Fisher Scientific) for differentiation under submerged conditions, following established protocols.8 For air−liquid interface (ALI) control cultures in cell culture inserts (Transwell, Corning), the basal medium was replaced with a PneumaCult-ALI medium, and the apical side of the cultures was air-exposed to promote differentiation. ..

Article Title: Retinoic acid regulates fetoplacental vascularization via notch signaling and a SEMA3E/F-PLEXIND1 axis
Article Snippet: .. Explants were cultured with DMSO for the control, or with retinoic acid (RA) (1 μM) (CAS #302-79-4; Sigma-Aldrich, St. Louis, MO), or DAPT (10 μM) (Cat #AAJ65864MA; Thermo Fisher Scientific, Waltham, MA) for 48 h, then treated with Click-iT® EdU Imaging Kit (Cat# C10337 ; Thermo Fisher Scientific, Waltham, MA) to assess proliferation and fixed by 4% PFA in PBS for 20 min. Allantoides were permeabilized and blocked for 30 min using 0.1% TritonTM X-100 (Cat# T8787; Sigma-Aldrich, St. Louis, MO) with 10% Normal Donkey Serum (NDS) (Cat# 0030-01; Southern Biotech, Birmingham, AL) in PBS. ..

Cell Isolation:

Article Title: Immortalization of human airway epithelial cells for genome editing applications
Article Snippet: Please see licensing information on first page for reuse rights. https://publications.ersnet.orgDownloaded from Glutamax 1 x 5 mL Thermo Fisher Scientific Penicilin/Streptomyc in (Pen/Strep) 1 x 5 mL Thermo Fisher Scientific Nasal cell basic medium Bronchial Epithelial Cell Medium (BEPICM) 50% ScienCell Advanced DMEM F12 44% Gibco B27 2% Thermo Fisher Scientific HEPES 1 M 10 mM Thermo Fisher Scientific Glutamax 1 x 5 mL Thermo Fisher Scientific Pen/Strep 1 x 5 mL Thermo Fisher Scientific Hydrocortisone 0,5 μg/ml Sigma-Aldrich N-acetyl-cysteine 1,25 mM Sigma-Aldrich Primocin 100 μg/ml InvivoGen A83-01 1 μM Tocris #2939/10 Epinephrine 0,5 μg/ml Sigma-Aldrich Y-27632 5 μM Selleck Chemicals #S1049 Nasal cell isolation medium Nasal cell basic medium R-spondin3 (RSPO3) 2% U-Protein Express Heregulin beta-1 50 nM PeproTech #100-03 Fibroblast Growth Factor (FGF) 10 100 ng/ml PeproTech #100-26 Hepatocyte Growth Factor (HGF) 25 ng/ml PeproTech #100-39 Amphotericin B 250 μg/ml Thermo Fisher Scientific Gentamycin 50 μg/ml Sigma-Aldrich Vancomycin 50 μg/ml Sigma-Aldrich Nasal cell expansion medium Nasal cell basic medium RSPO3 2% U-Protein Express on June 27, 2026 by guest. .. Please see licensing information on first page for reuse rights. https://publications.ersnet.orgDownloaded from Heregulin beta-1 5 nM PeproTech #100-03 DAPT 5 μM Thermo Fisher Scientific #15467109 FGF10 100 ng/ml PeproTech #100-26 HGF 25 ng/ml PeproTech #100-39 Rapamycin 50 nM Thermo Fisher Scientific Bronchial cell basic medium BEPICM 50 % ScienCell Advanced DMEM F12- 43,4 % Gibco HEPES 10 mM Thermo Fisher Scientific Glutamax 1 x Thermo Fisher Scientific Pen/Strep 1 x Thermo Fisher Scientific Primocin 100 μg/ml InvivoGen B27 2 % Thermo Fisher Scientific Hydrocortisone 0,5 μg/ml Sigma-Aldrich Triiodothyronine, T3 100 nM Sigma-Aldrich Epinephrine 0,5 μg/ml Sigma-Aldrich N-acetyl-cysteine 1,25 mM Sigma-Aldrich Nicotinamide 5 mM Sigma-Aldrich A83-01 1 μM Tocris #2939/10 DMH-1 1 μM Selleck Chemicals # S7146 Y-27632 5 μM Selleck Chemicals SB 202190 500 nM Sigma-Aldrich #S7067 Bronchial cell isolation medium Bronchial cell basic medium FGF10 100 ng/ml PeproTech #100-26 FGF7 25 ng/ml PeproTech #100-19 HGF 25 ng/ml PeproTech #100-39 Human Epidermal Growth Factor (hEGF) 5 ng/ml) PeproTech #100-15 Amphotericin B 250 μg/ml Thermo Fisher Scientific on June 27, 2026 by guest. .. Please see licensing information on first page for reuse rights. https://publications.ersnet.orgDownloaded from Gentamycin 50 μg/ml Sigma-Aldrich Vancomycin 50 μg/ml Sigma-Aldrich Bronchial cell expansion medium Bronchial cell basic medium RSPO3 2% U-Protein Express FGF10 (100 ng/ml) PeproTech #100-26 FGF7 (25 ng/ml) PeproTech #100-19 HGF (25 ng/ml) PeproTech #100-39 hEGF (5 ng/ml) PeproTech #100-15 DAPT 5 μg/ml Thermo Fisher Scientific #15467109 ALI-differentiation medium Advanced DMEM/F12 Gibco A83-01 50 nM Tocris #2939/10 hEGF 0.5 ng/ml PeproTech #100-15 Triiodothyronine, T3 100 nM Sigma-Aldrich Epinephrine 0.5 μg/ml Sigma-Aldrich TTNPB 100 nM Cayman Hydrocortisone 0.5 μg/ml Sigma-Aldrich Pen/Strep 1 % v/v Sigma-Aldrich ALI-differentiation medium supplements during air-exposure A83-01 500 nM Tocris #2939/10 DAPT 20 μM Thermo Fisher Scientific #15467109 DMH-1 5 μM Selleck Chemicals #S7146 Software and algorithms ICE analysis Ref. [10] on June 27, 2026 by guest.

Fluorescence:

Article Title: Low-Cost, Rapid Fabrication of Customizable Polyethylene Glycol-Based Cell Culture Devices.
Article Snippet: Biological research groups may face a high barrier to entry when constructing custom 3D cell culture devices to investigate multi-tissue interactions in vitro.. Standard fabrication methods such as lithography, etching, or molding are expensive and require specialized equipment and expertise.. To address this, we developed an accessible approach for producing polyethylene glycol (PEG)-based cell culture devices using stereolithography 3D printing with a polydimethylsiloxane intermediate mold.