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counterstaining with dapi  (Beyotime)


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    Structured Review

    Beyotime counterstaining with dapi
    Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi-counterstain/DAPI/pm41981465-107-32-35
    Average 99 stars, based on 32382 article reviews
    counterstaining with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: ‌Dual-target hUCMSCs/EVs therapy for autism spectrum disorder: remodeling gut microbiota and modulating neuroimmune crosstalk in a valproic acid-induced C57BL/6 mice model.
    Article Snippet: 352 Optical density measurements were performed using a SpectraMax M5 353 microplate reader (Molecular Devices). .. For immunofluorescence, sections were 354 stained with anti-Iba1 antibody (Sigma-Aldrich SAB2702364; 1:400) and DAPI 355 nuclear counterstain (Beyotime C1005; 1 μg/mL). ..

    Article Title: ‌Dual-target hUCMSCs/EVs therapy for autism spectrum disorder: remodeling gut microbiota and modulating neuroimmune crosstalk in a valproic acid-induced C57BL/6 mice model
    Article Snippet: Optical density measurements were performed using a SpectraMax M5 microplate reader (Molecular Devices). .. For immunofluorescence, sections were stained with anti-Iba1 antibody (Sigma-Aldrich SAB2702364; 1:400) and DAPI nuclear counterstain (Beyotime C1005; 1 μg/mL). ..

    Staining:

    Article Title: ‌Dual-target hUCMSCs/EVs therapy for autism spectrum disorder: remodeling gut microbiota and modulating neuroimmune crosstalk in a valproic acid-induced C57BL/6 mice model.
    Article Snippet: 352 Optical density measurements were performed using a SpectraMax M5 353 microplate reader (Molecular Devices). .. For immunofluorescence, sections were 354 stained with anti-Iba1 antibody (Sigma-Aldrich SAB2702364; 1:400) and DAPI 355 nuclear counterstain (Beyotime C1005; 1 μg/mL). ..

    Article Title: ‌Dual-target hUCMSCs/EVs therapy for autism spectrum disorder: remodeling gut microbiota and modulating neuroimmune crosstalk in a valproic acid-induced C57BL/6 mice model
    Article Snippet: Optical density measurements were performed using a SpectraMax M5 microplate reader (Molecular Devices). .. For immunofluorescence, sections were stained with anti-Iba1 antibody (Sigma-Aldrich SAB2702364; 1:400) and DAPI nuclear counterstain (Beyotime C1005; 1 μg/mL). ..

    Article Title: Single-cell sequencing reveals MCAM + MyCAFs as key pro-angiogenic cells interacting with endothelial cells in solid-type adenoid cystic carcinoma
    Article Snippet: .. Counterstain with DAPI (Beyotime) at room temperature for 5 min. After staining, remove the solution and wash with PBST three times. ..

    Incubation:

    Article Title: Icaritin eliminates tumor-associated macrophages via STX16-dependent extracellular vesicle delivery of autophagosomes from hepatocellular carcinoma cells
    Article Snippet: .. Samples were incubated with primary antibodies (Table S3) overnight at 4 °C, followed by fluorophore-conjugated secondary antibodies (1:500, 1 h, RT) and DAPI nuclear counterstain (Beyotime, C1002). .. Images were acquired using a Zeiss LSM800 confocal microscope.

    Article Title: Icaritin eliminates tumor-associated macrophages via STX16-dependent extracellular vesicle delivery of autophagosomes from hepatocellular carcinoma cells.
    Article Snippet: .. Samples were incubated with primary antibodies (Table S3) overnight at 4°C, followed by fluorophore-conjugated secondary antibodies (1:500, 1 h, RT) and DAPI nuclear counterstain (Beyotime, C1002). .. Images were acquired using a Zeiss LSM800 confocal microscope.



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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    Image Search Results


    (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Journal: Bioengineering & Translational Medicine

    Article Title: Extracellular matrix microarchitecture modulates cellular behavior and extracellular vesicle phenotypes in biomimetic tendon models

    doi: 10.1002/btm2.70134

    Figure Lengend Snippet: (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Article Snippet: Cellular morphology was assessed via actin staining (Invitrogen, Cat. No. A12379) and nuclear counterstaining with DAPI (Fisher Scientific, Cat. No. H‐1200‐10) ( n = 3).

    Techniques: Staining, Derivative Assay, Control