dapi nuclear staining (Beyotime)

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Beyotime dapi nuclear staining
Dapi Nuclear Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+nuclear+staining/DAPI/pm41981622-177-9-12
Average 99 stars, based on 31805 article reviews

dapi nuclear staining - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus.
Article Snippet: .. Cells were then incubated with FITC-conjugated goat anti-mouse IgG (1:1000; Cat. No.: A-11001; Thermo Fisher, USA), followed by DAPI nuclear staining (Cat. No.: C1005; Beyotime, Shanghai, China). ..

Article Title: Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus
Article Snippet: .. Cells were then incubated with FITC-conjugated goat anti-mouse IgG (1:1000; Cat. No.: A-11001; Thermo Fisher, USA), followed by DAPI nuclear staining (Cat. No.: C1005; Beyotime, Shanghai, China). ..

Article Title: Hydrogen gas inhibits neuroinflammation after traumatic brain injury by promoting microglia M2 polarization via AMPK-dependent mediation of HDAC5
Article Snippet: .. Samples were incubated with the following primary antibodies: mouse monoclonal anti-iNOS (Abcam, Cambridge, UK, # ab49999, 1:50) or rabbit monoclonal anti-TREM-2 (Abcam, # ab245227, 1:100) at 4°C overnight, washed three times with PBS, and then incubated with Cy3-conjugated (Proteintech, Rosemont, IL, USA, #SA00009-2, 1:200) or Page 11/29 uorescein (FITC)-conjugated (Proteintech, #SA00003-1, 1:200) secondary antibodies at 37°C for 1 h. After additional PBS washes, DAPI nuclear staining (Beyotime, #C1002) was performed at room temperature for 10 min. For immuno uorescence staining of BV2 cells, a similar protocol was utilized. .. Brie y, cells were xed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and then blocked with 10% FBS at 37°C for 1 h. Samples were incubated with rabbit polyclonal antiHDAC5 (Abcam, #ab 55403, 1:500) at 4°C overnight, washed three times with PBS, and incubated with Cy3-conjugated (Proteintech, #SA00009-2, 1:200) or FITC-conjugated (Proteintech, # SA00003-1, 1:200) secondary antibodies at 37°C for 1 h. After additional PBS washes, Hoechst nuclear staining (Beyotime, #C1017) was performed at room temperature for 15 min.

Staining:

Article Title: Novel genetic insights into causal effects of potential metformin targets and immune mediation on sepsis.
Article Snippet: .. After five rounds of primary and secondary antibodies incubations, DAPI nuclear staining (Beyotime, C1018, China) was performed at room temperature for 10 min. .. The following primary antibodies were used to perform immunostaining: anti-CD4 (Abcam, ab183685, rabbit monoclonal, 1:1000), anti-CD14 (CST, #93882, rabbit monoclonal, 1:500), anti-CD16a (S-RMab, S0B2184, rabbit monoclonal, 1:1000), anti-CD39 (CST, #14481, rabbit monoclonal, 1:400), and anti-CD127 (HuaBio, HA721214, rabbit monoclonal, 1:400).

Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response.
Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood.

Article Title: Ppm1b Negatively Regulates 3-Bromopyruvate Induced Necroptosis in Breast Cancer Cells
Article Snippet: Annexin-V FITC/PI staining (Nanjing keygen biotech, Nanjing) was used to detect the amounts of apoptosis cells by the method just described according to the manufacturer’s instructions. .. DAPI nuclear staining (Beyotime Institute of Biotechnology, Wuhan) assay was performed to examine the effects of 3-BP on cancer cell apoptosis. ..

Article Title: Inhibition of neutrophil infiltration and NETosis ameliorates sepsis-induced cardiac injury and reduces myocardial inflammation and apoptosis.
Article Snippet: Aims: Sepsis-induced cardiomyopathy (SIC) lacks effective targeted therapies.. This study investigated whether inhibiting neutrophil infiltration and neutrophil extracellular traps (NETosis) preserves cardiac function and mitigates inflammation and apoptosis in sepsis.. Methods: We performed analyses of gene-expression microarray datasets and single-cell RNA sequencing to define the SIC landscape, and generated cardiac RNA sequencing in LPS-treated versus control mice to validate microarray signatures.

TUNEL Assay:

Luciferase:

Reporter Gene Assay:

cDNA Synthesis:

Lysis:

Western Blot:

CCK-8 Assay:

Bicinchoninic Acid Protein Assay:

other:

Article Title: N-acetyl-L-cysteine ameliorates hepatocyte pyroptosis of dog type 1 diabetes mellitus via suppression of NLRP3/NF-κB pathway.
Article Snippet: Type 1 diabetes mellitus (T1DM) is a chronic and represented by insulin-causing pancreatic β-cell disruption and hyperglycemia.. N-Acetyl-Cysteine (NAC) is regarded as facilitating endothelial cell function and angiogenesis and may have treatment effect in the case of diabetes.. However, the impact of NAC on T1DM are unknown.

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