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dapi nuclear stain  (Dojindo Labs)


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    Structured Review

    Dojindo Labs dapi nuclear stain
    Dapi Nuclear Stain, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+staining/Cellstain+DAPI+solution/pm41923087-78-31-37
    Average 96 stars, based on 484 article reviews
    dapi nuclear stain - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease.
    Article Snippet: To evaluate nuclear translocation of NF-κB p65, fibroblasts (1×105 cells/well) from WT- or Tnfrsf8-deficient pSjD model mouse SG were first AR TI CL E IN P RE SS cultured in DMEM containing 10% FBS on collagen coated-culture cover glass (Matsunami Glass Ind., Ltd., Cat# CS1163) placed in a 24-well plate for one day.

    Staining:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: .. Tissues were incubated in 0.1% PBST containing Alexa Fluor 555- Phalloidin (1/500, A34055, Thermo Fisher Scientific) and DAPI (1/2000, D523, Dojindo Laboratories) for 30 min. After staining, samples were mounted on glass slides and imaged using a LSM800 confocal microscope (Zeiss). ..

    Incubation:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: .. Tissues were incubated in 0.1% PBST containing Alexa Fluor 555- Phalloidin (1/500, A34055, Thermo Fisher Scientific) and DAPI (1/2000, D523, Dojindo Laboratories) for 30 min. After staining, samples were mounted on glass slides and imaged using a LSM800 confocal microscope (Zeiss). ..

    Article Title: Lipid A counteracts doxorubicin-induced systemic dysfunction by boosting mitochondrial activity
    Article Snippet: PE-labeled anti-mouse CD4 antibody (Cat# 130310, BioLegend, San Diego, CA, USA) and Alexa Fluor 700-labeled anti-mouse/human CD45R/B220 antibody (Cat# 103231, BioLegend, San Diego, CA, USA) were added and incubated overnight at 4°C. .. After washing with PBST, Alexa Fluor 568-labeled goat anti-rat IgG secondary antibody (Cat# 2379471, Invitrogen, Carlsbad, CA, USA) and DAPI (Cat# 340-07971, Dojindo Molecular Technologies, Inc., Kumamoto, Japan) were added, and the samples were incubated at room temperature for 1 hour. .. After washing, the samples were sealed using FluoroKeeper (Cat# 12593-64, Nacalai Tesque, Kyoto, Japan).

    Microscopy:

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: .. Tissues were incubated in 0.1% PBST containing Alexa Fluor 555- Phalloidin (1/500, A34055, Thermo Fisher Scientific) and DAPI (1/2000, D523, Dojindo Laboratories) for 30 min. After staining, samples were mounted on glass slides and imaged using a LSM800 confocal microscope (Zeiss). ..



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    Image Search Results


    (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Journal: bioRxiv

    Article Title: Lymphatic vessel dysfunction contributes to severe dengue pathogenesis

    doi: 10.64898/2026.03.27.714698

    Figure Lengend Snippet: (A) Schematic representation of an OrganoPlate® 3-lane tissue chip formed by two channels for cell seeding and a middle channel for gel loading. (B) Brightfield image and (C) confocal projection of HUVECs (red) seeded on top channel and HDLECs (green) seeded in bottom channel. Images taken after 24 h of culture under flow conditions. (D) Representative confocal max projection of HDLECs cultured in flow for 14 days in OrganoPlate® and treated for 24 h with DENV-2 NS1 (NS1) or control medium (untreated=Unt). Nuclei stained with DAPI. Merged images shows VE-cadherin (green) and F-actin (red) and DAPI (blue). Scale bar = 30 µm.

    Article Snippet: The slides were then incubated with the nuclear stain DAPI (cat# D9542, Merck) at 1:1000 dilution for 10 min and mounted onto glass slides using mounting medium (InvitrogenTM Fluoromount-GTM Mounting Medium-00495802).

    Techniques: Cell Culture, Control, Staining