cyt997 (MedChemExpress)

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MedChemExpress cyt997
Fig. 1 <t>CYT997</t> inhibited proliferation and colony formation of GC cells. a Chemical structural of CYT997. b-d SGC-7901, MKN45, AGS and BGC-823 cells were treated with CYT997 for 24 and 48 h. Cell proliferation was detected by a CCK8 assay b and a colony formation assay c and d. *p < 0.05; **p < 0.01

Fig. 1 <t>CYT997</t> inhibited proliferation and colony formation of GC cells. a Chemical structural of CYT997. b-d SGC-7901, MKN45, AGS and BGC-823 cells were treated with CYT997 for 24 and 48 h. Cell proliferation was detected by a CCK8 assay b and a colony formation assay c and d. *p < 0.05; **p < 0.01

Cyt997, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyt997/Lexibulin/pm32576206-64-0-14
Average 91 stars, based on 2 article reviews

cyt997 - by Bioz Stars, 2026-09

91/100 stars

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1) Product Images from "Mitochondrial ROS accumulation inhibiting JAK2/STAT3 pathway is a critical modulator of CYT997-induced autophagy and apoptosis in gastric cancer."

Article Title: Mitochondrial ROS accumulation inhibiting JAK2/STAT3 pathway is a critical modulator of CYT997-induced autophagy and apoptosis in gastric cancer.

Journal: Journal of experimental & clinical cancer research : CR

doi: 10.1186/s13046-020-01621-y

Fig. 1 CYT997 inhibited proliferation and colony formation of GC cells. a Chemical structural of CYT997. b-d SGC-7901, MKN45, AGS and BGC-823 cells were treated with CYT997 for 24 and 48 h. Cell proliferation was detected by a CCK8 assay b and a colony formation assay c and d. *p < 0.05; **p < 0.01

Figure Legend Snippet: Fig. 1 CYT997 inhibited proliferation and colony formation of GC cells. a Chemical structural of CYT997. b-d SGC-7901, MKN45, AGS and BGC-823 cells were treated with CYT997 for 24 and 48 h. Cell proliferation was detected by a CCK8 assay b and a colony formation assay c and d. *p < 0.05; **p < 0.01

Techniques Used: CCK-8 Assay, Colony Assay

Fig. 2 CYT997 induced cell cycle G2/M arrest and apoptosis in GC cells. a The cell cycle distribution of SGC-7910 and MKN45 cells treated with CYT997 was analyzed by flow cytometry.**p < 0.01. b Expression of Cyclin B1 and p21 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997. c Following treatment of SGC-7901 and MKN45 cells in different dose of CYT997 for 24 h, apoptotic cells were detected by Annexin V-FITC and PI double staining. **p < 0.01.d Expression of cleaved caspase 3, caspase 3, cleaved PARP and PARP was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997

Figure Legend Snippet: Fig. 2 CYT997 induced cell cycle G2/M arrest and apoptosis in GC cells. a The cell cycle distribution of SGC-7910 and MKN45 cells treated with CYT997 was analyzed by flow cytometry.**p < 0.01. b Expression of Cyclin B1 and p21 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997. c Following treatment of SGC-7901 and MKN45 cells in different dose of CYT997 for 24 h, apoptotic cells were detected by Annexin V-FITC and PI double staining. **p < 0.01.d Expression of cleaved caspase 3, caspase 3, cleaved PARP and PARP was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997

Techniques Used: Flow Cytometry, Expressing, Western Blot, Double Staining

Fig. 3 CYT997 triggered autophagy in SGC-7901 and MKN45 cells. a After transient transfection with the GFP-fused LC3 plasmid, SGC-7901 and MKN45 cells were treated with CYT997 for 24 h. Cells were incubated with LysoTracker Red DND-99. The distribution of GFP-LC3B expression and LysoTracker Red was visualized by confocal microscopy. Scale bars = 7.5 μm. Results were validated by three separate experiments. b Expression of LC3B and Beclin-1 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997. c SGC-7901 and MKN45 cells were treated with CYT997 alone or in combination with 3-MA. Cell apoptosis was detected by flow cytometry. **p < 0.01.d Expression of cleaved PARP, PARP, cleaved caspase 3 and caspase 3 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997 alone or in combination with 3-MA

Figure Legend Snippet: Fig. 3 CYT997 triggered autophagy in SGC-7901 and MKN45 cells. a After transient transfection with the GFP-fused LC3 plasmid, SGC-7901 and MKN45 cells were treated with CYT997 for 24 h. Cells were incubated with LysoTracker Red DND-99. The distribution of GFP-LC3B expression and LysoTracker Red was visualized by confocal microscopy. Scale bars = 7.5 μm. Results were validated by three separate experiments. b Expression of LC3B and Beclin-1 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997. c SGC-7901 and MKN45 cells were treated with CYT997 alone or in combination with 3-MA. Cell apoptosis was detected by flow cytometry. **p < 0.01.d Expression of cleaved PARP, PARP, cleaved caspase 3 and caspase 3 was detected by western blotting in SGC-7901 and MKN45 cells treated with CYT997 alone or in combination with 3-MA

Techniques Used: Transfection, Plasmid Preparation, Incubation, Expressing, Confocal Microscopy, Western Blot, Flow Cytometry

Fig. 5 Mitochondrial ROS inhibitor MitoQ reversed the inhibition of CYT997 in GC cells. a SGC-7901 cells were treated with CYT997 alone or in combination with MitoQ (500 nM). Cell viability was detected by a CCK8 assay. b The cell cycle distribution of SGC-7901 cells treated with CYT997 alone or in combination with MitoQ (500 nM) was analyzed by flow cytometry. c SGC-7901 cells were treated with CYT997 alone or in combination with MitoQ (500 nM). Apoptosis was detected by flow cytometry. d Expression of cell cycle, autophagy and apoptosis related proteins was detected by western blotting in SGC-7901 cells

Figure Legend Snippet: Fig. 5 Mitochondrial ROS inhibitor MitoQ reversed the inhibition of CYT997 in GC cells. a SGC-7901 cells were treated with CYT997 alone or in combination with MitoQ (500 nM). Cell viability was detected by a CCK8 assay. b The cell cycle distribution of SGC-7901 cells treated with CYT997 alone or in combination with MitoQ (500 nM) was analyzed by flow cytometry. c SGC-7901 cells were treated with CYT997 alone or in combination with MitoQ (500 nM). Apoptosis was detected by flow cytometry. d Expression of cell cycle, autophagy and apoptosis related proteins was detected by western blotting in SGC-7901 cells

Techniques Used: Inhibition, CCK-8 Assay, Flow Cytometry, Expressing, Western Blot

Fig. 8 Mechanism of CYT997-induced apoptosis in GC cells. CYT997 activated mitochondrial ROS to trigger protective autophagy and inhibited JAK2/STAT3 pathway to induce G2/M arrest and apoptosis in GC

Figure Legend Snippet: Fig. 8 Mechanism of CYT997-induced apoptosis in GC cells. CYT997 activated mitochondrial ROS to trigger protective autophagy and inhibited JAK2/STAT3 pathway to induce G2/M arrest and apoptosis in GC

Techniques Used:

CCK-8 Assay:

Article Title: Mitochondrial ROS accumulation inhibiting JAK2/STAT3 pathway is a critical modulator of CYT997-induced autophagy and apoptosis in gastric cancer
Article Snippet: CYT997 (MF: C24H30N6O2, MW: 434.53, purity: 99.46%), IL-6 and Mitoquinone (MitoQ) were bought from MCE (Shanghai, China).

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cyt997 (MedChemExpress)

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