cyp24a1 (OriGene)
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OriGene
cyp24a1
Cyp24a1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyp24a1/CYP24A1+Rabbit+Polyclonal+Antibody/pmc12720125-304-15-16
Average 94 stars, based on 1 article reviews
Cyp24a1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyp24a1/CYP24A1+Rabbit+Polyclonal+Antibody/pmc12720125-304-15-16
Average 94 stars, based on 1 article reviews
cyp24a1 - by Bioz Stars,
2026-11
94/100 stars
Images
1) Product Images from "Artemisitene triggers calcium-dependent ferroptosis by disrupting the LSH-EWSR1 interaction in colorectal cancer"
Article Title: Artemisitene triggers calcium-dependent ferroptosis by disrupting the LSH-EWSR1 interaction in colorectal cancer
Journal: Redox Biology
doi: 10.1016/j.redox.2025.103950
Figure Legend Snippet: Analysis of ATT-mediated calcium regulation via RNA-seq. (A) The analysis of the KEGG pathway for ATT-treated cells (10 μM). (B) Heatmap showing the relative expression of differentially expressed genes in HCT-116 cells with or without ATT treatment (10 μM). (C) Volcano plots of DEGs in ATT vs. control HCT-116 cells. |Fold change| > 2 and P < 0.05 were shown in red (upregulated) or blue (downregulated). (D, E) Cellular calcium level of CRC cells in HCT-116 and HT-29 cells following ATT treatment (0, 5, 10, 20 μM) for 24 h. CRC cells were treated with ATT (0, 5, 10, 20 μM) for 24 h. The influence of ATT on the RNA (F) and protein levels (G, H) of the differentially expressed protein CYP24A1 were assessed. (I) The immunofluorescence analysis of CYP24A1 expression in CRC cells with ATT treatment (10 μM) for 24 h. Scale bar: 50 μm. Data are expressed as mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA followed by Dunnett's post hoc test. P -values are labeled directly in figure. P < 0.05 was considered statistically significant, and P ≥ 0.05 was considered non-significant.
Techniques Used: RNA Sequencing, Expressing, Control, Immunofluorescence, Labeling
Figure Legend Snippet: The crucial role of LSH in ATT-induced ferroptosis in HCT-116. HCT-116 cells were pre-treated with siLSH (A) or Flag-LSH (B) for 24 h and then treated with or without ATT (10 μM) for 24 h. Cytotoxicity of ATT in cells was tested. Flow cytometry was performed to evaluate oxidation-dependent DCF fluorescence (C) , C11-BODIPY-detected lipid peroxidation (D) , and intracellular Ca 2+ levels (E) in CRC cells with LSH knockdown after ATT treatment. HCT-116 cells were pre-treated with Flag-LSH for 24 h and then treated with or without ATT (10 μM) for 24 h. Flow cytometry was used to analyze the effects of ATT on intracellular oxidative activity (DCFH-DA) (F) , lipid peroxidation (C11-BODIPY) (G) , and calcium levels (H) in CRC cells with LSH overexpression. Effects of ATT on target protein LSH and downstream protein CYP24A1 upon LSH knockdown (I) and LSH overexpression (J) . Data are expressed as mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA followed by Tukey's post hoc test. P -values are labeled directly in figure. P < 0.05 was considered statistically significant, and P ≥ 0.05 was considered non-significant.
Techniques Used: Flow Cytometry, Fluorescence, Knockdown, Activity Assay, Over Expression, Labeling
Figure Legend Snippet: Identification of LSH-interacting proteins perturbed by ATT. HCT-116 cells were treated with ATT (10 μM) for 24 h. (A) LC-MS/MS analysis of LSH-immunoprecipitated proteins in HCT-116 cells (left). Representative peptide counts and molecular weights of indicated proteins are shown (right). (B) Co-immunoprecipitation (Co-IP) analysis of the interaction between LSH and EWSR1 in CRC cells. (C) Immunofluorescence co-localization analysis of EWSR1 and LSH in CRC cells. Scale bar: 5 μm. (D) HCT-116 cells were pre-treated with Flag-LSH for 24 h and then treated with or without ATT (10 μM) for 24 h. Co-IP analysis of interactions between Flag-tagged wild-type LSH, mutant, and EWSR1. (E) HCT-116 cells were treated with ATT (0, 5, 10, 20 μM) for 24 h. Protein levels of EWSR1 in CRC cells were determined. (F) HCT-116 cells were treated with siEWSR1 for 48 h. Effect of EWSR1 knockdown on HCT-116 cell viability. Influence of EWSR1 knockdown on lipid peroxidation (C11-BODIPY) (G) and intracellular calcium levels (H). Statistical significance was assessed using one-way ANOVA followed by Dunnett's post hoc test. Impact of EWSR1 knockdown on (I) RNA and (J) protein expression of LSH and CYP24A1. Statistical significance was assessed using two-way ANOVA followed by Dunnett's post hoc test. (K) HCT-116 cells were transfected with siEWSR1 for 48 h, and a dual-luciferase assay was performed to examine its effect on the promoter activity of CYP24A1 mediated by exogenous LSH. Statistical significance was assessed using one-way ANOVA followed by Tukey's post hoc test. Data are expressed as mean ± SD (n = 3). P -values are labeled directly in figure. P < 0.05 was considered statistically significant, and P ≥ 0.05 was considered non-significant.
Techniques Used: Liquid Chromatography with Mass Spectroscopy, Immunoprecipitation, Co-Immunoprecipitation Assay, Immunofluorescence, Mutagenesis, Knockdown, Expressing, Transfection, Luciferase, Activity Assay, Labeling
Figure Legend Snippet: The effect of ATT on CRC progression in vivo . (A) Representative images of tumor samples from each group (n = 6). (B) Measurement of tumor volume and (C) weight (n = 6). (D) HE staining of tumor tissues (Scale bar: 100 μm) and IHC detection of Ki-67, CYP24A1, SCD, and LSH expression in tumor tissues (Scale bar: 50 μm). (E) Protein expression levels of CYP24A1, SCD, and LSH in tumor tissues were determined. (F) Impact of ATT on the interaction of LSH and EWSR1 in vivo was assayed by Co-IP assay. Statistical significance was assessed using one-way ANOVA followed by Dunnett's post hoc test. P -values are labeled directly in figure. P < 0.05 was considered statistically significant, and P ≥ 0.05 was considered non-significant.
Techniques Used: In Vivo, Staining, Expressing, Co-Immunoprecipitation Assay, Labeling
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