cyp1b1 (Proteintech)
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Structured Review
Proteintech
cyp1b1
Cyp1b1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyp1b1/CYP1B1+Antibody/pmc12948649-121-59-60
Average 95 stars, based on 51 article reviews
Cyp1b1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyp1b1/CYP1B1+Antibody/pmc12948649-121-59-60
Average 95 stars, based on 51 article reviews
cyp1b1 - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "oxPAPC ‐Mediated lncRNA CYP1B1 ‐ AS1 From Dendritic Cells Accelerates Atherosclerosis"
Article Title: oxPAPC ‐Mediated lncRNA CYP1B1 ‐ AS1 From Dendritic Cells Accelerates Atherosclerosis
Journal: Journal of Cellular and Molecular Medicine
doi: 10.1111/jcmm.71066
Figure Legend Snippet: The screening and evaluation of DC‐derived lncRNA. (A, B) The plasma levels of oxPAPC and IL‐1β in CHD. (C) The up‐regulated lncRNAs under various oxPAPC stimuli. (D) The down‐regulated lncRNAs under various oxPAPC stimuli. (E) The expression level of the ENST00000628135 (CYP1B1‐AS1). (F) The genomic localisation of mouse for Gm33055 and CYP1B1‐AS1 by Integrative Genomics Viewer (IGV). (G) The genomic localisation of human for Gm33055 and CYP1B1‐AS1 by IGV. (H) The intersection of Gm33055 and RNA‐binding proteins in the promoter region of CYP1B1‐AS1 by RBP map analysis. (I) The non‐coding potential of Gm33055 by Coding‐Non‐Coding Index (CNCI) prediction. (J) The non‐coding potential of Gm33055 by Coding Potential Calculator 2 (CPC2) prediction (** P < 0.01, *** P < 0.001, **** P < 0.0001).
Techniques Used: Derivative Assay, Clinical Proteomics, Expressing, RNA Binding Assay
Figure Legend Snippet: The relationship between CYP1B1‐AS1, CYP1B1 and AS. (A) Calculation of the Pearson correlation coefficient between CYP1B1 and CYP1B1‐AS1. (B) The CYP1B1 expression levels after knockdown and over‐expression of m‐CYP1B1‐AS1 by immunoblotting analysis. (C) The success of knockdown and over‐expression CYP1B1‐AS1 (m‐CYP1B1‐AS1) in mouse by PCR validation. (D) The subsets and distribution of PTPRC+ CYP1B1+ cells by Dimplot (Seurat). (E) The differentially expressed genes among cell subpopulations (Coloured point: Differentially expressed genes, log 2 FoldChange > 0.25 and adjusted p ‐value < 0.01). (F, G) The differential number of interactions and interaction strength in CellChat (* p < 0.05, *** p < 0.001, **** p < 0.0001).
Techniques Used: Expressing, Knockdown, Over Expression, Western Blot, Biomarker Discovery
Figure Legend Snippet: The expression levels of CD14, TLR‐4 signalling pathway and CYP1B1 by rt‐PCR and Western Blot. (A–F) The transcription levels of Gm33055, CD14, Caspase‐11, Caspase‐1, IL‐1β, and IL‐18 by rt‐PCR. (G) The expression levels of CD14, TLR‐4 signalling molecules, cell death‐related molecules, and CYP1B1 by Western Blot (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot
Figure Legend Snippet: The mechanism of CYP1B1‐AS1 on AS. (A) The subcellular localisation of h‐CYP1B1‐AS1 and m‐CYP1B1‐AS1 by fluorescence in situ hybridization (FISH). (B) The transcription factors binding sites in the promoters of human and mouse CYP1B1 and CYP1B1‐AS1 by JASPAR. (C) The common transcription factor binding motif for human and mouse CYP1B1 and CYP1B1‐AS1. (D, E) The levels of DC nuclear proteins after oxPAPC stimulation by TMT mass spectrometry. (F) The levels of nuclear and cytoplasmic NFATc2 before and after oxPAPC stimulation by Western blot (** p < 0.01).
Techniques Used: Fluorescence, In Situ Hybridization, Binding Assay, Mass Spectrometry, Western Blot
Figure Legend Snippet: The relationship among oxPAPC, NFATC2, and CYP1B1‐AS1. (A–D) The enrichment of NFATC2 before and after oxPAPC stimulation by ChIP‐Seq. (A) The genomic distribution of enriched peaks. (B) The length distribution of enriched peaks. (C) The distribution of reads around transcription start sites. (D) NFATC2 enrichment in the promoter region sites and sequence information of CYP1B1‐AS1. (E) The binding of CYP1B1‐AS1 to NFATC2 by RNA pulldown assay. (F, G) The expression levels of Nfatc2 and CYP1B1‐AS1 in mouse DCs following Nfatc2 knockdown or overexpression were determined by RT‐PCR. (H) The CYP1B1 expression levels after knockdown and overexpression of Nfatc2 by immunoblotting analysis (* P < 0.05, ** P < 0.01, *** P < 0.001).
Techniques Used: ChIP-sequencing, Sequencing, Binding Assay, Expressing, Knockdown, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot
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