pe rabbit anti human cyp19a1  (Bioss)

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Distinct Luteinization Profiles of Cultured Human Granulosa Cells From Small Antral and Preovulatory Follicles

doi: 10.1210/clinem/dgaf218

Figure Lengend Snippet: Gene expression (RT-qPCR). (A) Granulosa cells from small antral follicles, (B) granulosa-lutein cells from preovulatory follicles. Gene expression values for the corpus luteum are shown in yellow. No significant differences were observed between control and androstenedione-treated groups. Granulosa cells from small antral follicles initially exhibit high relative expression of CYP19A1 and HSD17β1 , key genes in estrogen production, with a decline observed after 6-12 hours. In contrast, LHCGR , STAR , and CYP11A1 show an opposite trend, with increased expression after 24 to 48 hours. The relative mRNA expression of the proliferation marker MKI67 is notably higher in granulosa cells from small antral follicles compared to granulosa-lutein cells from preovulatory follicles. Granulosa-lutein cells from preovulatory follicles display a decrease in CYP19A1 and HSD17β1 expression after 12 to 24 hours, followed by an increase in HSD17β1 at 96 hours. LHCGR expression increases steadily from 12 to 96 hours, while STAR expression is initially high, decreases at 12 to 24 hours, and then stabilizes. CYP11A1 expression increases after 36 hours and continues to rise until 96 hours. Significant P values are indicated as follows: * P ≤ .05, ** P ≤ .01, *** P ≤ .001.

Article Snippet: Human CYP19A1 , Hs00903411_m1 , 4331182.

Techniques: Gene Expression, Quantitative RT-PCR, Control, Expressing, Marker

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Distinct Luteinization Profiles of Cultured Human Granulosa Cells From Small Antral and Preovulatory Follicles

doi: 10.1210/clinem/dgaf218

Figure Lengend Snippet: Gene expression and hormone analysis. (A) FSHR and CYP19A1 gene expression in granulosa-lutein cells from preovulatory follicles. (B) 17 β -Estradiol levels in spent culture media from granulosa-lutein cells treated as control (100 nM testosterone) or with 25 ng/mL FSH and 100nM testosterone. (A) No significant differences (n.s.) were observed in the mRNA expression of FSHR and CYP19A1 between control and FSH-treated granulosa-lutein cells over 12 days. (B) 17 β -Estradiol secretion showed no significant differences (n.s.) between control and FSH-treated groups at any of the 3 time points (days 4, 8, and 12). Significant P values are indicated as follows: * P ≤ .05, ** P ≤ .01, *** P ≤ .001.

Article Snippet: Human CYP19A1 , Hs00903411_m1 , 4331182.

Techniques: Gene Expression, Control, Expressing

Journal: Frontiers in Veterinary Science

Article Title: MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4 -mediated ferroptosis

doi: 10.3389/fvets.2025.1621738

Figure Lengend Snippet: D19 restoring LPS-suppressed steroid hormone production in GCs. (A) ELISA measurement of A 4 , E 2 , and P 4 levels in GCs. (B,C) qRT-PCR and Western blotting were employed to detect the relative expression levels of mRNA and protein, respectively, for genes involved in steroid hormone synthesis ( HSD17B4 , CYP19A1 , 3β-HSD , CYP11A1 , and STAR ). Data from at least three independent experiments are presented as mean ± SEM. Significant differences ( p < 0.05) are denoted by different letters.

Article Snippet: CYP19A1 , bs-0114R , Bioss , Rabbit , 1:500.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Expressing

Journal: Frontiers in Veterinary Science

Article Title: MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4 -mediated ferroptosis

doi: 10.3389/fvets.2025.1621738

Figure Lengend Snippet: D19 preventing GPX4 deficiency-induced disruption of steroidogenesis in GCs. (A) Levels of E 2 and P 4 in different treatment groups were measured using ELISA. (B) The relative mRNA and protein expression levels of steroid hormone synthesis-related genes ( HSD17B4 , CYP19A1 , 3β-HSD , CYP11A1 , and STAR ) were detected by qRT-PCR and Western blotting, respectively. All experiments were performed in triplicate, and data are presented as mean ± SEM. Different letters indicate statistically significant differences ( p < 0.05).

Article Snippet: CYP19A1 , bs-0114R , Bioss , Rabbit , 1:500.

Techniques: Disruption, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: Molecular Metabolism

Article Title: Amygdala aromatase controls food intake, reward, and thermoregulation

doi: 10.1016/j.molmet.2025.102202

Figure Lengend Snippet: Quantitative and histological identification of aromatase-expressing cells in rat amygdala. Both male and female amygdala expressed high levels of the mRNA of the aromatase gene, CYP19a1; the second highest expression levels of CYP19a1 were found in the hypothalamus, whereas the lowest levels were found in the non-limbic brain region, the nucleus of the solitary tract (NTS) (A) . In the central amygdala (CeA), we found similar levels of aromatase expression between female and male rats (B) . Virally-mediated labelling of aromatase-expressing neurons in the amygdala, using a vector expressing Cre under the aromatase rat promoter combined with a vector introducing mCherry only in Cre-expressing cells, indicated that the highest expression of aromatase in the amygdala was located within the CeA, with medium density of cells also present in the basolateral amygdala (BLA) (C) . Higher magnification images, highlighting the dense expression of aromatase-producing cells in the CeA (D) . Fluorescent in situ hybridization indicates that aromatase producing cells (green) in the amygdala of male and female rats co-express the neuronal marker NeuN (red) (E). Anatomical markers in the images correspond to bregma −2.52 in Paxinos and Watson’s Rat Brain Atlas, fifth edition. F = females; M = males; NTS = nucleus of the solitary tract; AMYG = amygdala; BLA = basolateral amygdaloid nucleus, anterior part. Data are expressed as mean ± SEM. ∗ p < 0.05.

Article Snippet: Gene expression was measured with real-time quantitative PCR (RT-PCR) using the following TaqMan assays for rat tissues: Cyp19a1-Rn00567222_m1 (Applied Biosystems).

Techniques: Expressing, Plasmid Preparation, In Situ Hybridization, Marker

Journal: Molecular Metabolism

Article Title: Amygdala aromatase controls food intake, reward, and thermoregulation

doi: 10.1016/j.molmet.2025.102202

Figure Lengend Snippet: Loss of aromatase in the CeA impairs energy balance in a sex-specific manner. Representative image of a brain section belonging to a rat subjected to an intra-amygdala cannulation; schematic representation of the placement of the cannula to the left and coronal brain section including the trace of the cannula aimed at amygdala to the right (A) . Intra-amygdala injection of AAV-CYP19a1siRNA resulted in a 60% reduction of aromatase expression in the CeA, compared to controls injected with AAV-ScrambledsiRNA (B) . Loss of amygdala aromatase resulted in a small but significant cumulative body-weight change in intact females (C). The knockdown also resulted in a significant increase in cumulative chow intake, measured over 8 weeks, in CYP-KD females (D) . In contrast, no effect on body weight (E) or food intake (F) was found in CYP-KD males. When metabolically challenged by a free choice of HFHS and chow, the loss of CeA aromatase resulted in weight gain (G) and hyperphagia (H) in females. On the other hand, body weight (I) or food intake (J) was not affected by the CYP-KD knockdown in males. The greater calorie intake in CYP-KD females, was likely due to a combination of an increased chow and lard intake (K) , while no changes were detected in males (L) . Increased visceral adiposity was also observed in CYP-KD females, as a robust increase in gonadal white adipose tissue was detected in CYP-KD females (M) . No change in adiposity was identified in males (N). Fasting blood glucose levels were not altered in females ( O) , but fasting glucose levels were reduced in males under an obesogenic challenge ( P ). Anatomical markers in image A corresponds to bregma −2.76 in Paxinos and Watson’s Rat Brain Atlas, fifth edition. F = females; M = males; CYP-KD = Cyp19a1 knockdown; BW = body weight; HFHS diet = high-fat high-sugar diet. N = 9–11 for females, N = 11–12 for males. Data are expressed as mean ± SEM. # p < 0.1, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Gene expression was measured with real-time quantitative PCR (RT-PCR) using the following TaqMan assays for rat tissues: Cyp19a1-Rn00567222_m1 (Applied Biosystems).

Techniques: Injection, Expressing, Knockdown, Metabolic Labelling

Journal: Molecular Metabolism

Article Title: Amygdala aromatase controls food intake, reward, and thermoregulation

doi: 10.1016/j.molmet.2025.102202

Figure Lengend Snippet: Effects of CeA aromatase loss on energy balance in gonadectomized male and female rats. Loss of amygdala aromatase did not alter cumulative body weight gain in OVX females (A) . Furthermore, CeA aromatase loss did not affect cumulative food intake compared to controls (B), Surprisingly CeA CYP-KD was associated with increased body temperature, likely driven by increased brown adipose tissue (BAT) temperature, compared to controls (C). No changes in cumulative body weight ( D ), chow intake (E), or body temperature (F) were detected in ORX males between AAV groups. Locomotor activity was not altered in OVX or ORX CYP-KD rats (G) . However, aromatase loss led to increased weight gain (H) and food intake (I) in OVX female rats challenged with an obesogenic diet. In contrast, body weight (J) and food intake (K) were not affected by the aromatase loss in ORX males, compared to controls, under the obesogenic diet. The hyperphagia that developed as a result of CeA aromatase knockdown was primarily driven by increased intake compared to controls, during the dark cycle in females (L) . Meal size was increased after CeA CYP-KD (M) and there was also a trend to increased meal duration (N) compared to controls, but no change in meal number was found (O) . Trace of cumulative hourly food intake in OVX control and CYP-KD rats (P) . In contrast, loss of CeA aromatase did not affect food intake during the light or dark in ORX males compared to controls, when fed a high-fat diet (HFD) (Q) . CeA CYP-KD did not affect meal size (R) , meal duration (S) , or meal number (T) compared to controls, in ORX males. Trace of cumulative hourly food intake in ORX control and CYP-KD rats (U) . OVX = ovariectomized females; ORX = orchiectomized males; HFD = high fat diet; CYP-KD = Cyp19a1 knockdown. N = 12–13, per sex and per treatment. Data are expressed as mean ± SEM. ∗ p < 0.05.

Article Snippet: Gene expression was measured with real-time quantitative PCR (RT-PCR) using the following TaqMan assays for rat tissues: Cyp19a1-Rn00567222_m1 (Applied Biosystems).

Techniques: Activity Assay, Knockdown, Control

Journal: Molecular Metabolism

Article Title: Amygdala aromatase controls food intake, reward, and thermoregulation

doi: 10.1016/j.molmet.2025.102202

Figure Lengend Snippet: Loss of CeA aromatase results in altered food-reward behavior in a sex-specific manner. When offered palatable chocolate pellets, CYP-KD OVX female rats consumed more over a 2 h period than controls (A) . Chronic aromatase expression knockdown led to increased motivation to work for a palatable food reward compared to control, as indicated by increased number of active lever presses (B) and an increase in pellets earned in the progressive ratio operant conditioning test (C). The number of entries into the food dispenser (D) and locomotor activity in the operant conditioning box (E) remained unchanged by the knockdown. Male rats did not change their palatable chocolate pellet consumption after CeA aromatase loss (F). Food-motivated behavior was not altered by CeA aromatase knockdown in ORX males as indicated by unaltered number of active lever presses (G) and food rewards earned (H) , compared to control males. Food seeking (I) and locomotor activity (J) also remained unchanged between AAV groups in ORX males. When expressed as % response of controls, KD female rats worked twice as hard for the food reward under loss of CeA aromatase compared to KD males (K) , which did not change their food-motivated behavior compared to male controls. Application of exogenous E2 into the CeA of intact female rats, with normal levels of CeA aromatase, resulted in reduced effort for a food reward (L) and reduced food rewards earned (M), in addition to reduced food seeking (N) compared to controls , without alterations in locomotor activity (O) . The same exogenous E2 application did not alter active lever presses (P) , food rewards earned (Q) , food seeking (R), or locomotor activity (S) compared to controls, in male rats. OVX = ovariectomized females; ORX = orchiectomized males; CYP-KD = Cyp19a1 knockdown, E2 = 17 b-estradiol. A–K: n = 10–14, L–O: n = 10, P–S: n = 7. Data are expressed as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: Gene expression was measured with real-time quantitative PCR (RT-PCR) using the following TaqMan assays for rat tissues: Cyp19a1-Rn00567222_m1 (Applied Biosystems).

Techniques: Chocolate, Expressing, Knockdown, Control, Activity Assay