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cycloheximide chx  (MedChemExpress)


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    Structured Review

    MedChemExpress cycloheximide chx
    Cycloheximide Chx, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclohexamide/(S)-Indoximod/pm42834166-373-18-20
    Average 94 stars, based on 3 article reviews
    cycloheximide chx - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Lysis:

    Article Title: Axin1 regulates tooth root development by inhibiting AKT1-mTORC1 activation and Shh translation in Hertwig's epithelial root sheath.
    Article Snippet: In brief, HERS-H1 cells were treated with 250 nM Torin1 or DMSO as control for 4 h after transfected with Axin1 siRNA or the negative control (Scramble; GenePharma) for 36 h as mentioned above. .. Cells were treated with 100 μg ml−1 cyclohexamide (CHX; MedChemExpress, HY12320) 10 min before lysis, washed in ice-cold PBS plus 100 μg ml−1 CHX at 4°C and then lysed in polysome lysis buffer [0.3 M NaCl, 15 mMMgCl2, 15 mM Tris-HCl (pH 7.6), 1% Triton X-100, 0.1 U μl−1 RNAsin (Yeasen Biotechnology), 100 μg ml−1 CHX, 1 μg ml−1 heparin] at 4°C. ..

    Transfection:

    Article Title: Imbalance in ALR ubiquitination accelerates the progression of nonalcoholic steatohepatitis to hepatocellular carcinoma
    Article Snippet: .. After transfection for 24 h, cyclohexamide (CHX; MedChemExpress; HY-12320, Monmouth Junction, NJ, USA) was added to cells at a nal concentration of 50 μg/mL to block new protein synthesis. ..

    Article Title: CHIP Drives Proteasomal Degradation of Nur77 to Alleviate Oxidative Stress and Intrinsic Apoptosis in Cisplatin-induced Nephropathy
    Article Snippet: .. Polyethylenimine was used for transfection of 293t cells, a highly transfectable cell line.. Cyclohexamide (50μg ml-1, MCE, catalog HY-12320) was used to treat the cells for 2-8 hours, while the treatment with 15μM of MG132 (MCE, catalog HY-13259) was carried out for 2-12 hours. .. For CHIP activation, 2μM YL109 (MCE, catalog HY-18619) was used to pre-treat the BUMPT cells for 8 hours, and they were co-treated with cisplatin.

    Concentration Assay:

    Article Title: Imbalance in ALR ubiquitination accelerates the progression of nonalcoholic steatohepatitis to hepatocellular carcinoma
    Article Snippet: .. After transfection for 24 h, cyclohexamide (CHX; MedChemExpress; HY-12320, Monmouth Junction, NJ, USA) was added to cells at a nal concentration of 50 μg/mL to block new protein synthesis. ..

    Article Title: TRIM21/USP15 balances ACSL4 stability and the imatinib resistance of gastrointestinal stromal tumors.
    Article Snippet: BACKGROUND: Imatinib has become an exceptionally effective targeted drug for treating gastrointestinal stromal tumors (GISTs).. Despite its efficacy, the resistance to imatinib is common in GIST patients, posing a significant challenge to the effective treatment.. METHODS: The expression profiling of TRIM21, USP15, and ACSL4 in GIST patients was evaluated using Western blot and immunohistochemistry.

    Blocking Assay:

    Article Title: Imbalance in ALR ubiquitination accelerates the progression of nonalcoholic steatohepatitis to hepatocellular carcinoma
    Article Snippet: .. After transfection for 24 h, cyclohexamide (CHX; MedChemExpress; HY-12320, Monmouth Junction, NJ, USA) was added to cells at a nal concentration of 50 μg/mL to block new protein synthesis. ..

    Article Title: TRIM21/USP15 balances ACSL4 stability and the imatinib resistance of gastrointestinal stromal tumors.
    Article Snippet: BACKGROUND: Imatinib has become an exceptionally effective targeted drug for treating gastrointestinal stromal tumors (GISTs).. Despite its efficacy, the resistance to imatinib is common in GIST patients, posing a significant challenge to the effective treatment.. METHODS: The expression profiling of TRIM21, USP15, and ACSL4 in GIST patients was evaluated using Western blot and immunohistochemistry.

    Mutagenesis:

    Article Title: A rare mutation (p.Ala264Thr) of GATA4 is responsible for atrial septal defect and pulmonary valve stenosis.
    Article Snippet: The GATA4-Flag-pEnter plasmids, both WT and mutated, were transfected into the AC16 cells for 36 hours using LipofectamineTM 2000 CD Transfection Reagent (Thermo Fisher Scientific, USA). .. Then, Cyclohexamide (CHX, 100μg/mL, MedChemExpress, HY-12320) was added into the cells to assess the pathogenicity of the mutation via Western blot analysis. .. For the Western blot, cells were lysed on ice using RIPA buffer (Solarbio, China) and rotated for 2 hours at 4°C.

    Western Blot:

    Article Title: A rare mutation (p.Ala264Thr) of GATA4 is responsible for atrial septal defect and pulmonary valve stenosis.
    Article Snippet: The GATA4-Flag-pEnter plasmids, both WT and mutated, were transfected into the AC16 cells for 36 hours using LipofectamineTM 2000 CD Transfection Reagent (Thermo Fisher Scientific, USA). .. Then, Cyclohexamide (CHX, 100μg/mL, MedChemExpress, HY-12320) was added into the cells to assess the pathogenicity of the mutation via Western blot analysis. .. For the Western blot, cells were lysed on ice using RIPA buffer (Solarbio, China) and rotated for 2 hours at 4°C.



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