anti cx3cr1 rabbit antibody (Proteintech)

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Proteintech anti cx3cr1 rabbit antibody
Anti Cx3cr1 Rabbit Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cx3cr1/CX3CR1+Antibody/pm41672915-99-18-22
Average 95 stars, based on 63 article reviews

anti cx3cr1 rabbit antibody - by Bioz Stars, 2026-10

95/100 stars

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Incubation:

Article Title: Systems-level molecular and immunological evidence identifies Th17/Treg modulation as a key mechanism of CRSJ’s neuroprotection in Parkinson’s disease
Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Servicebio, No. G6045). .. Membranes were blocked with 5% non-fat milk at room temperature for 1.5 h, followed by overnight incubation at 4°C with primary antibodies against RORγt (Affinity, No. DF3196), CX3CR1 (Proteintech, No.60339), α-synuclein (Servicebio, No. GB11773), Foxp3 (Servicebio, No. GB112325 ), IL-17A (No. GB11110), Arg1 (Servicebio, No. GB11285), iNOS (Servicebio, No. GB153965 ), TGF-β (Servicebio, No. GB111876 ), smad3 (Servicebio, No. GB150085 ), and β-actin (Servicebio, No. GB15003). .. Membranes were then incubated with appropriate secondary antibodies for 1 h (Servicebio, No. GB23303, GB23301).

Article Title: Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis
Article Snippet: .. For immunohistochemical staining, 3% (v/v) H 2 O 2 was used to quench the endogenous peroxidase activity, and primary antibodies against TCF7L2 , THBS1, CCR2a, CX3CR1 (13838-1-AP, 18304-1-AP, 16153-1-AP, 13885-1-AP, Proteintech, Wuhan, China), and Lubricin (ER1912-53, Huabio, Hangzhou, China) were incubated overnight at 4 °C. .. After washing, the sections were incubated with Alexa Fluor 555 Antibody Labeling Kits (A20187, ThermoFisher Scientific), CoraLite488-conjugated goat anti-rabbit IgG and CoraLite594-conjugated goat anti-mouse IgG (SA00013-2, SA00013-3, Proteintech, Wuhan, China).

Article Title: Potentiated fractalkine signaling axis affects neuroinflammation in 1,2-dichloroethane- intoxicated mice via divergent modulating ways on microglial hyperactivation.
Article Snippet: .. The protein samples were transferred onto a PVDF membrane and incubated at 4◦C overnight with the primary antibody (1:1000) against FKN (Cat. #ab25088), A1R (Cat. #ab82477), A2aR (Cat. #ab3461) obtained from Abcam, Cambridge, UK; sFKN (Cat. #14–7986–81), purchased from Thermo Fisher Scientific, Waltham, MA, USA; Iba-1 (Cat. #WL02406), Arg-1 (Cat. #WL02825), glial fibrillary acidic protein (GFAP) (Cat. #WL0836), Interleukin-6 (IL-6) (Cat. #WL02841), inducible nitric oxide synthase (iNOS) (Cat. #WL0992a), p-p38 (Cat. #WLP1576), p-IκB (Cat. #WL02495), protein kinase A (PKA) (Cat. # WL04408), obtained from Wanleibio, Shenyang, China; BDNF (Cat. #T55577), obtained from Abmart, Berkeley, USA; CX3CR1 (Cat. #13885–1-AP), tumor necrosis factor-α (TNF-α) (Cat. #60291–1-Ig) or Complement 3 (C3) (Cat. #21337–1-AP), obtained from Proteintech, Rosemont, IL, USA; CD11b (Cat. #YT5923), Complement 1q (C1q) (Cat. #YN0612), transforming growth factor-β (TGF-β) (Cat. #YT4632), p38 (Cat. #YP0338), p65 (Cat. #YP0188), p-p65 (Cat. #YT2002), adenylate cyclase (AC) (Cat. #YN1621), or IL-10 (Cat. # YM8444), obtained from ImmunoWay, Texas, USA; and GAPDH (as an internal control, Cat. #10494–1-AP, Proteintech, Rosemont, IL, USA), respectively. .. Afterwards, the target bands on the blot were hatched with the secondary antibody and then detected with the ECL plus kit (Thermo Fisher Scientific, Waltham, MA, USA), which were photographed using Azure c500.

Article Title: Experimental study on the role and biomarker potential of CX3CR1 in osteoarthritis
Article Snippet: .. Overnight incubation at 4 °C was conducted with primary antibodies against GAPDH (1:5000, Proteintech, China), CX3CR1 (1:2000, Proteintech, China), and MMP13 (1:2000, Proteintech, China). .. After three PBST washes, membranes were incubated for one hour at room temperature with Multi-rAb TM HRP-Goat Antirabbit Recombinant Secondary Antibody (H + L) (1:5000, Proteintech, China).

Article Title: Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis.
Article Snippet: .. For immunohistochemical staining, 3% (v/v) H2O2 was used to quench the endogenous peroxidase activity, and primary antibodies against TCF7L2, THBS1, CCR2a, CX3CR1 (13838-1-AP, 18304- 1-AP, 16153-1-AP, 13885-1-AP, Proteintech, Wuhan, China), and Lubricin (ER1912-53, Huabio, Hangzhou, China) were incubated overnight at 4 °C. .. After washing, the sections were incubated with Alexa Fluor 555 Antibody Labeling Kits (A20187, ThermoFisher Scientific), CoraLite488-conjugated goat anti-rabbit IgG and CoraLite594-conjugated goat anti-mouse IgG (SA00013-2, SA00013-3, Proteintech, Wuhan, China).

Article Title: Experimental study on the role and biomarker potential of CX3CR1 in osteoarthritis.
Article Snippet: .. Overnight incubation at 4 °C was conducted with primary antibodies against GAPDH (1:5000, Proteintech, China), CX3CR1 (1:2000, Proteintech, China), and MMP13 (1:2000, Proteintech, China). .. After three PBST washes, membranes were incubated for one hour at room temperature with Multi-rAbTM HRP-Goat Antirabbit Recombinant Secondary Antibody (H + L) (1:5000, Proteintech, China).

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other:

Article Title: CX3CL1/CX3CR1 axis dysregulation contributes to epileptogenic mechanisms in focal cortical dysplasia.
Article Snippet: Membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against CX3CL1 (1:1000; #29282, Signalway Antibody), CX3CR1 (1:1000; #38481, Signalway Antibody), ARG1 (1:1000; #sc-271430, Santa Cruz), iNOS (1:1000; #sc-7271, Santa Cruz), TNF-α (1:1000; #17590-1-AP, Proteintech), CD206 (1:1000; #18704-1-AP, Proteintech), β-actin (1:1000; #3700, CST), and GAPDH (1:1000; #5174, CST).

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