ct26wt (ATCC)
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99
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ct26wt
Ct26wt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ct26wt/product/ATCC
Average 99 stars, based on 3500 article reviews
Ct26wt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ct26wt/product/ATCC
Average 99 stars, based on 3500 article reviews
ct26wt - by Bioz Stars,
2026-02
99/100 stars
Images
1) Product Images from "RNAi Screening in Tumor Cells Identifies Artificial microRNAs That Improve Oncolytic Virus Replication"
Article Title: RNAi Screening in Tumor Cells Identifies Artificial microRNAs That Improve Oncolytic Virus Replication
Journal: Pharmaceuticals
doi: 10.3390/ph18050708
Figure Legend Snippet: Enrichment of specific amiRNA sequences after serial passage of SINV library in tumor cells. ( A ) SINV titers obtained after 1 passage (P1) and 4 serial passages (P4) in CT26WT tumor cells infected at multiplicity of infection (MOI) of 0.1 for 48 h ( n = 5). Data are presented as means ± standard error of the mean (SEM). Student’s unpaired, one-tailed t -test was performed; **: p < 0.01. ( B ) Stacked area plot of percent frequency of each amiRNA in SINV–amiRNA library before passaging (P0) and after 4 rounds of serial passage (P4) in CT26WT cells. Cumulative percent frequencies of all 5 replicates are shown for P4. Each color represents an individual amiRNA. ( C ) Heat map showing percent frequency of reads of the 10 most enriched amiRNAs in each replicate at P0 (input) and after P4.
Techniques Used: Infection, One-tailed Test, Passaging
Figure Legend Snippet: Enriched amiRNAs increase SINV replication. ( A ) Fluorescent images (4× magnification) and ( B ) virus titers from CT26WT cells transfected with siRNAs corresponding to amiRNA-1 and -2 targeting sequences and subsequently infected with SINV-GFP at MOI of 0.1. Fluorescent images were taken, and supernatants were collected 48 h post-infection ( n = 4). ( C ) Virus-induced cytotoxicity revealed by Coomassie Blue stain of CT26WT cells transfected with siRNAs corresponding to identified hits and subsequently infected with SINV-GFP. Cells were fixed and stained 72 h post-infection. ( D ) Enrichment of amiRNA-expressing SINV from competition experiments against parental SINV-GFP virus. The percentage of each virus is shown before competition (P0) and after 4 rounds of competition (P4) in CT26WT cells ( n = 3). Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; **: p < 0.01; ***: p < 0.001.
Techniques Used: Virus, Transfection, Infection, Staining, Expressing, One-tailed Test
Figure Legend Snippet: The identified amiRNAs increase the replication of oncolytic VSVΔ51, MG1, and VVdd. ( A ) Fluorescent images (4× magnification) and virus titers from CT26WT cells transfected with siRNAs corresponding to the identified hits and subsequently infected with VSVΔ51-YFP (top panel, n = 6), MG1-GFP (middle panel, n = 3), or VVdd-mCherry (bottom panel, n = 5). Images were taken and supernatants were collected 48 h post-infection for VSVΔ51 and MG1 and at 72h for VVdd. ( B ) Virus-induced cytotoxicity as measured by AlamarBlue viability assay of CT26WT cells transfected with siRNAs corresponding to identified hits and subsequently infected with VSVΔ51-YFP. Cell viability was measured 72 h post-infection ( n = 3). ( C ) Dual-luciferase reporter assay. BHK cells were transfected with the indicated reporter plasmids possessing target sites for enriched amiRNAs in 3′ UTR of Renilla luciferase reporter and subsequently infected with the indicated VSV viruses. Luciferase activity was measured 24 h post-infection. ( n = 3). ( D ) Enrichment of amiRNA-expressing VSVΔ51 from competition experiments against parental virus VSVΔ51-YFP. The frequency of each virus is shown at P0 and P4 in CT26WT cells ( n = 3). Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; *: p < 0.05; **: p < 0.01; ***: p < 0.001.
Techniques Used: Virus, Transfection, Infection, Viability Assay, Luciferase, Reporter Assay, Activity Assay, Expressing, One-tailed Test
Figure Legend Snippet: siRNA-1 and -2 do not target the IFN pathway. ( A ) IFN-β levels in CT26WT cells transfected with the indicated siRNA and then infected with VSVΔ51 at an MOI of 0.001 for 24h, as measured by qPCR ( n = 2) and ( B ) ELISA ( n = 2). Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; *: p < 0.05; **: p < 0.01. ( C ) Relative expression of various ISGs after siRNA transfection and infection of CT26WT cells with VSVΔ51 at an MOI of 0.001 for 24h, as measured by qPCR ( n = 3). Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; *: p < 0.05; **: p < 0.01.
Techniques Used: Transfection, Infection, Enzyme-linked Immunosorbent Assay, One-tailed Test, Expressing
Figure Legend Snippet: Gene expression changes induced by siRNA-1 and siRNA-2 reveals marked overlap of differentially expressed genes. ( A ) Microarray analysis of changes in gene expression 16h after VSVΔ51 infection of CT26WT transfected with siRNA-1 or siRNA-2. Heat map shows genes that were differentially expressed at least 3.5-fold compared with cells treated with control siRNA and infected with VSVΔ51. ( B ) Venn diagram showing number and overlap of differentially expressed genes (≥2-fold) in VSVΔ51-infected, siRNA-1- or siRNA-2-transfected cells, compared with control siRNA. Up arrows and down arrows indicate an increase and a decrease in expression, respectively. ( C ) qPCR analysis of selected genes identified as downregulated by microarray analysis of siRNA-treated CT26WT cells ( n = 3). Data are presented as means ± SEM. One-way ANOVA was performed with Dunnett’s multiple comparisons post hoc test. Compared with control siRNA, ##: p < 0.01; ###: p < 0.001. Compared with control siRNA + VSV, *: p < 0.05; **: p < 0.01; ***: p < 0.001. ( D ) Knockdown of target gene promotes VSV replication in CT26WT cells. CT26WT cells were transfected with the indicated siRNAs, and 48 h post-transfection, they were infected with VSVΔ51. Supernatants were collected 48 h post-infection, and virus was quantified ( n = 3). Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; compared with control siRNA, *: p < 0.05; **: p < 0.01; ***: p < 0.001.
Techniques Used: Gene Expression, Microarray, Infection, Transfection, Control, Expressing, Knockdown, Virus, One-tailed Test
Figure Legend Snippet: The amiRNA-expressing viruses show enhanced replication and efficacy in vivo. ( A ) Quantification of SINV in tumors ( n = 6) and spleens ( n = 3) 48 h post-intratumoral treatment of CT26WT tumor-bearing mice with 1 × 10 8 PFU. Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; *: p < 0.05. ( B ) Quantification of VSVΔ51 in tumors ( n = 14) and spleens ( n = 3) 48 h post-intravenous treatment of CT26WT tumor-bearing mice with 5 × 10 8 PFU. Data are presented as means ± SEM. Student’s unpaired, one-tailed t -test was performed; *: p < 0.05. ( C ) Tumor volumes of mice ( n = 6) bearing CT26WT subcutaneous tumors treated intravenously on day 1 and intratumorally on days 2 and 3 with 5 × 10 8 PFU of the indicated viruses. Values represent means ± SEM. Two-way ANOVA test was performed with Dunnett’s multiple comparisons post hoc test; ***: p < 0.001.
Techniques Used: Expressing, In Vivo, One-tailed Test
