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anti crls1 antibody  (Proteintech)


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    Proteintech anti crls1 antibody
    Knockdown of <t>cardiolipin</t> <t>synthase</t> <t>1</t> <t>(CRLS1)</t> attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.
    Anti Crls1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti crls1 antibody/product/Proteintech
    Average 93 stars, based on 13 article reviews
    anti crls1 antibody - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Anti-alcoholism drug disulfiram inhibits PANoptosis by blocking mitochondrial permeabilization in macrophages"

    Article Title: Anti-alcoholism drug disulfiram inhibits PANoptosis by blocking mitochondrial permeabilization in macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1726408

    Knockdown of cardiolipin synthase 1 (CRLS1) attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.
    Figure Legend Snippet: Knockdown of cardiolipin synthase 1 (CRLS1) attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.

    Techniques Used: Knockdown, Binding Assay, Expressing, Comparison, Western Blot, Transfection, Control, Staining, Membrane, Fluorescence, Microscopy



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    Image Search Results


    Knockdown of cardiolipin synthase 1 (CRLS1) attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Anti-alcoholism drug disulfiram inhibits PANoptosis by blocking mitochondrial permeabilization in macrophages

    doi: 10.3389/fimmu.2025.1726408

    Figure Lengend Snippet: Knockdown of cardiolipin synthase 1 (CRLS1) attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.

    Article Snippet: The anti-PNPT1 antibody and anti-CRLS1 antibody (14845-1-AP) were obtained from Proteintech (Rosemont, IL, USA).

    Techniques: Knockdown, Binding Assay, Expressing, Comparison, Western Blot, Transfection, Control, Staining, Membrane, Fluorescence, Microscopy